Immunocytochemistry analysis of Phospho-RUNX2 (Ser28) in HepG2 cells (4h of LPS treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-RUNX2 (Ser28) polyclonal antibody (Product # PA5-105643) 1:200 and mouse anti-beta tubulin antibody (1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).