Fig 1: Endothelial immunosuppression is enhanced by TNFR2 but not TNFR1. A Representative histograms and quantification of PD-L1 expression by sEND.1 cells following administration of either human or mouse TNFα with mouse IFNγ; n = 3. B Representative histograms and quantification of PD-L1 expression by ECs incubated with IFNγ, TNFα and anti-TNFR2; n = 3. C Representative histograms and quantification of PD-L1 expression by sEND.1 cells incubated with IFNγ, TNFα, and anti-TNFR1; n = 3. D Proliferation of OT-I CD8+ T cells detected by FCM using the FITC channel after incubation with IFNγ-stimulated sEND.1 cells treated by TNFα with or without anti-TNFR2. E Quantification of CD8+ T cell proliferation; n = 3. **P < 0.01, ***P < 0.001, ****P < 0.0001; ns, not significant