HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PLSCR2 (Cat# RC214936, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLSCR2 (Cat# TA810855)(1:500). Positive lysates [LY412529] (100ug) and [LC412529] (20ug) can be purchased separately from OriGene.