Anti-Nestin antibody [10C2] - Neural Stem Cell Marker ab22035 from Abcam

Product Specs
ItemAnti-Nestin antibody [10C2] - Neural Stem Cell Marker
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab22035
HostMouse, Nude mouse
IsotypeIgG1
Quantity100 UG
ClonalityMonoclonal
ApplicationsImmunocytochemistry, Western blot
Clone10C2
Gene NameNES
TargetNestin
ReactivityHuman (Hu)
Concentration1 mg/ml
Conjugate/TagUnconjugated
Antibody TypePrimary

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Nuclear Glycoprotein A Repetitions Predominant (GARP) Is a Common Trait of Glioblastoma Stem-like Cells and Correlates with Poor Survival in Glioblastoma PatientsCancers (Basel)December 5, 2023Niklas Zimmer, Emily R. Trzeciak, Andreas Müller, Philipp Licht, Bettina Sprang, Petra Leukel, Volker Mailänder, Clemens Sommer, Florian Ringel, Jochen Tuettenberg, et al.Mainz as described before [7]. The following antibodies were used in the study: anti-nestin (Abcam, ab22035), anti-GARP (Origene, AP17415PU-N), and secondary antibodies goat anti-mouse Alexa Fluor 488 or goat

(2) An Efficient and Cost-Effective Approach to Generate Functional Human Inducible Pluripotent Stem Cell-Derived AstrocytesCellsSeptember 26, 2023Gonzalez H, Narasipura SD, Shull T, Shetty A, Teppen TL, Naqib A, Al-Harthi L.NPCs were routinely validated for their specific markers, Pax-6 (Abcam: ab5790) and Nestin (Abcam: ab22035). NPCs were differentiated into astrocytes as described [31]. Briefly, NPCs were dissociated to single

(3) Microglia-secreted TNF-α affects differentiation efficiency and viability of pluripotent stem cell-derived human dopaminergic precursorsPLOS ONESeptember 26, 2023Shirley D. Wenker, María Isabel Farias, Victoria Gradaschi, Corina Garcia, Juan Beauquis, María Celeste Leal, Carina Ferrari, Xianmin Zeng, Fernando J. Pitossinumbers Anti-GFAP,DAKO,Z0334 Anti-MHC-II,Serotec,MCA46G Anti-ED1,Serotec,MAC341R Anti-SOX1,R&D system,AF3369 Anti-Nestin,Abcam,ab22035 Anti-TUJ1,Promega,G712A Anti-MAP-2,Cell Signaling,9043S Anti-FOXA-2,Abcam,ab117542 Anti-LMX1a,Abcam,ab31006 Anti-

(4) Differentiation of peripheral sensory neurons from iPSCs derived from stem cells from human exfoliated deciduous teeth (SHED)Frontiers in Cell and Developmental BiologyJuly 13, 2023Nathalia C. Oliveira, Fabiele B. Russo, Patricia C. B. Beltrão-Bragaantibody,Abcam,Cat#Ab77095 Anti-SOX2 antibody,Cell Signaling Technology,Cat# 2748 "Anti-nestin antibody, mouse",Abcam,Cat#ab22035 "Anti-peripherin antibody, rabbit",Abcam,Cat#ab246502 "Anti-synapsin I antibody, rabbit",Abcam,Cat#ab1543 "Anti-MAP2

(5) Three-dimensional co-culture platform of human induced pluripotent stem cell-derived oligodendrocyte lineage cells and neurons for studying myelinationSTAR ProtocolsJune 1, 2023Martina von der Bey, Silvia De Cicco, Susanne Zach, Bastian Hengerer, Ebru Ercan-HerbstBasic Protein Antibody (rat) (1:50),Sigma-Aldrich,MAB386 Anti-Nestin antibody (mouse) (1:200),Abcam,ab22035 Anti-Olig-2 antibody (rabbit) (1:200),Sigma-Aldrich,AB9610 Anti-PAX6 antibody (rabbit) (1:100),Invitrogen,42-6600 Anti-Tau

(6) Generation of four human induced pluripotent stem cell lines from COVID-19 hospitalized patients with increased levels of cardiac Troponin in the acute infection phase developing or not myocarditisStem Cell ResearchMarch 1, 2023Martina Rabino, Erica Rurali, Chiara Zamboni, Davide Rovina, Sara Mallia, Matteo Cauteruccio, Andrea Baggiano, Carlo Maria Giacari, Milena Bellin, Giulio Pompilio,Thermo Fisher Scientific Cat# PA5-49431,RRID: AB_2634885 Differentiation Markers,Mouse anti-NESTIN,1:150,Abcam Cat# ab22035,RRID: AB_446723 Differentiation Markers,Rabbit anti-PAX6,1:300,BioLegend Cat# PRB-278P,RRID: AB_291612 Secondary

(7) Spatial transcriptomics reveals niche-specific enrichment and vulnerabilities of radial glial stem-like cells in malignant gliomasNature communicationsFebruary 23, 2023Ren Y, Huang Z, Zhou L, Xiao P, Song J, He P, Xie C, Zhou R, Li M, Dong X, et al.with 5% milk in PBS for 1 hour at RT, cells were incubated with either anti-hNESTIN (1:2000; Abcam, ab22035), anti-Sox2 (1:1000; Abcam, ab92494), or anti-Pax6 (1:500; Abcam, ab5790), anti-mCherry (1:2000; Abcam,

(8) Loss of phosphatase CTDNEP1 potentiates aggressive medulloblastoma by triggering MYC amplification and genomic instabilityNature communicationsFebruary 10, 2023Luo Z, Xin D, Liao Y, Berry K, Ogurek S, Zhang F, Zhang L, Zhao C, Rao R, Dong X, et al.captured using a Nikon C2 + confocal microscope. Primary antibodies used were: Nestin (Mouse, Abcam; Cat#ab22035, 1:500), Ki67 (Rabbit, Thermo Fisher; Cat#MA5-14520, 1:1000), BrdU (Mouse, BD Bioscience; Cat#347580,

(9) Increased cysteine metabolism in PINK1 models of Parkinson's diseaseDisease Models & MechanismsJanuary 1, 2023Marco Travaglio, Filippos Michopoulos, Yizhou Yu, Rebeka Popovic, Edmund Foster, Muireann Coen, L. Miguel Martinsthawing). Antibodies and dyes Primary antibodies and dyes employed in this study were anti-nestin (Abcam, cat. no. ab22035, 1:1000), anti-Sox2 (ThermoFisher Scientific, cat. no. 149811-82, 1:200), anti-Pax6 (Biolegend, cat.

(10) Nuclear accumulation of host transcripts during Zika Virus InfectionPLoS pathogensJanuary 1, 2023Leon KE, Khalid MM, Flynn RA, Fontaine KA, Nguyen TT, Kumar GR, Simoneau CR, Tomar S, Jimenez-Morales D, Dunlap M, et al.anti-FLAG (Abcam, ab18230), anti-Actin (CST, 4967S), anti-GFAP (Abcam, ab53554), anti-Nestin (Abcam, ab22035) and anti-Beta III tubulin (Abcam, ab18207). Secondary antibodies used include goat anti-rabbit Alexa

(11) Dysfunctional Extracellular Matrix Remodeling Supports Perianal Fistulizing Crohn′s Disease by a Mechanoregulated Activation of the Epithelial-to-Mesenchymal TransitionCellular and Molecular Gastroenterology and HepatologyJanuary 1, 2023Giulia Rizzo, Federica Rubbino, Sudharshan Elangovan, Giusy Sammarco, Sara Lovisa, Restelli Restelli, Samuel Pineda Pineda Chavez, Luca Massimino, Luigi Lamparelli, Marianna Paulis, et al.Kingdom), anti-NANOG (1:500; ab62734; Abcam), anti-SOX-2 (1:200; ab97959; Abcam), anti-Nestin (1:200; ab22035; Abcam), anti-α-smooth muscle actin (1:100; ab5694; Abcam), and anti-GATA4 (1:150; ab134057; Abcam).

(12) Patient-derived three-dimensional cortical neurospheres to model Parkinson’s diseasePLOS ONEDecember 1, 2022Waseem K. Raja, Esther Neves, Christopher Burke, Xin Jiang, Ping Xu, Kenneth J. Rhodes, Vikram Khurana, Robert H. Scannevin, Chee Yeun Chungneuronal stem cell markers (PAX6 (Invitrogen, Cat. No. 426600, 1:200 dilution) and Nestin (Abcam, Cat. No. ab22035, 1:500 dilution)), as well as the neural crest markers SOX10 (R&D Systems, Cat. No. AF2864, 1:20 dilution)

(13) Multi-omic profiling reveals the ataxia protein sacsin is required for integrin trafficking and synaptic organizationCell reportsNovember 1, 2022Romano LEL, Aw WY, Hixson KM, Novoselova TV, Havener TM, Howell S, Taylor-Blake B, Hall CL, Xing L, Beri J, et al.RRID:AB_2250373 KDEL,Enzo Life Sciences,ADI-SPA-827-D MAP2,Abcam,Ab5392; RRID:AB_2138153 NES,Abcam,Ab22035; RRID:AB_446723 NEUN,Millipore,ABN90P; RRID:AB_2341095 NFH,Abcam,Ab8135; RRID:AB_306298 NUP98,Cell Signaling

(14) Increased apoptotic sensitivity of glioblastoma enables therapeutic targeting by BH3-mimeticsCell Death & DifferentiationOctober 1, 2022Anna L. Koessinger et al.Caspase 3 (Cell Signaling #9664), PARP1 (Cell Signaling #9532) and SOX2 (Abcam #ab92494), NESTIN (Abcam #ab22035), GFAP (Santa Cruz #SC-6170) at 4 °C overnight in blocking buffer. α-tubulin (Sigma #T5168, 1:5000),

(15) Establishment of autaptic culture with human-induced pluripotent stem cell-derived astrocytesiScienceAugust 19, 2022Uchino K, Tanaka Y, Kawaguchi S, Kubota K, Watanabe T, Katsurabayashi S, Hirose S, Iwasaki K.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Mouse anti-Nestin monoclonal antibody,Abcam,"Cat# ab22035, RRID: AB_446723" DACH1 rabbit polyclonal antibody,Proteintech,Cat# 10914-1-AP Mouse anti-GFAP monoclonal

(16) Junctional instability in neuroepithelium and network hyperexcitability in a focal cortical dysplasia human modelBrainJune 30, 2022Simoni H Avansini et al.anti-N-Cadherin (BD; Clone 32; 1:150); rabbit anti-NeuN (Abcam; ab128886; 1:1000); mouse anti-NESTIN (Abcam; ab22035; 1:200); and rabbit anti-Ki67 (Abcam; ab15580; 1:100). The primary antibodies were incubated overnight

(17) AAV2-mediated gene therapy for Bietti crystalline dystrophy provides functional CYP4V2 in multiple relevant cell modelsScientific reportsJune 9, 2022Wang JH, Lidgerwood GE, Daniszewski M, Hu ML, Roberts GE, Wong RCB, Hung SSC, McClements ME, Hewitt AW, Pébay A, et al.Santa Cruz Biotechnology), mouse anti-TRA-1-60 (1 μg/ml, MA1-023-PE, Thermo Fisher Scientific), mouse anti-NESTIN (10 μg/ml, AB22035, Abcam), rabbit anti-alpha-fetoprotein (AFP, 10 μg/ml, st1673, Sigma-Aldrich), mouse anti-smooth muscle

(18) Transcription Factor 4 loss-of-function is associated with deficits in progenitor proliferation and cortical neuron contentNature communicationsMay 2, 2022Papes F, Camargo AP, de Souza JS, Carvalho VMA, Szeto RA, LaMontagne E, Teixeira JR, Avansini SH, Sánchez-Sánchez SM, Nakahara TS, et al.ab54210; clone [2D9A12]; 1:1000); rabbit anti-SOX3 (Abcam; ab183606; 1:200); mouse anti-Nestin (Abcam; ab22035; clone [10C2]; 1:1000); goat anti-SOX17 (R&D Systems; AF1924; 1:200); rabbit anti-Brachyury (Sigma;

(19) Three-dimensional-engineered bioprinted in vitro human neural stem cell self-assembling culture model constructs of Alzheimer's diseaseBioactive MaterialsMay 1, 2022Yi Zhang, Haiyan Chen, Xiaoyan Long, Tao Xuprovided with the microscope. The primary antibodies used in this study included anti-Nestin antibody (ab22035, Abcam); anti-SOX2 antibody (ab93689, Abcam); anti-β-tubulin type III (Tuj1, ab78078, Abcam); anti-MAP2

(20) Astrocytes Derived from Familial and Sporadic Alzheimer's Disease iPSCs Show Altered Calcium Signaling and Respond Differently to Misfolded Protein TauCellsApril 22, 2022Brezovakova V, Sykova E, Jadhav S.ab64581; Abcam), PAX-6 (1:1000; ab195045; Abcam), SOX-2 (1:500; ab92494; Abcam), and Nestin (1:500; ab22035; Abcam). 2.4. Calcium Imaging Cells were first loaded with fluo-4 AM (1 μM; Thermo-Scientific, Bratislava,

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5/21/2020Anti-Nestin Stains Tissue Very Well Samantha LanjewarThe anti-Nestin antibody works very strongly on tissue slices.

Anti-Nestin antibody [10C2] - Neural Stem Cell Marker from Abcam

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Anti-Nestin antibody [10C2] - Neural Stem Cell Marker from Abcam

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