HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ST13 (RC220533, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ST13 rab. Positive lysates LY418342 (100ug) and LC418342 (20ug) can be purchased separately from OriGene.