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Anti-alpha Tubulin antibody - Microtubule Marker
Anti-alpha Tubulin antibody - Microtubule Marker from Abcam
(1)
Write a review
Citations:
(308)
Supplier Page
Supplier Page from
Abcam for
Anti-alpha Tubulin antibody - Microtubule Marker
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-alpha Tubulin antibody - Microtubule Marker
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab18251
Antibody Type
Primary
Quantity
50 UG
Isotype
IgG
Host
Rabbit
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western blot
Clonality
Polyclonal
Conjugate/Tag
Unconjugated
Concentration
0.3 mg/ml
Gene Name
TUBA4A
Reactivity
Human, Mouse, Rat
Target
alpha Tubulin
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (308)
(101)
Phosphodiesterase 2A2 regulates mitochondria clearance through Parkin-dependent mitophagy
Communications biology
October 21, 2020
Lobo MJ, Reverte-Salisa L, Chao YC, Koschinski A, Gesellchen F, Subramaniam G, Jiang H, Pace S, Larcom N, Paolocci E,
et al
.
1:1000
;
Abcam
,
ab62341
,
1:1000
), SAMM50 (
Atlas Antibodies
,
HPA042935
,
1:1000
) and α-Tubulin (
Abcam
,
ab18251
,
1:5000
);
horseradish
-peroxidase-conjugated
goat
anti-
mouse
or anti-rabbit secondary antibodies were
(102)
Tumor Treating Fields (TTFields) Hinder Cancer Cell Motility through Regulation of Microtubule and Acting Dynamics
Cancers
October 17, 2020
Voloshin T, Schneiderman RS, Volodin A, Shamir RR, Kaynan N, Zeevi E, Koren L, Klein-Goldberg A, Paz R, Giladi M,
et al
.
donkey
-serum blocking solution for 1 h, and then stained with anti-tubulin antibody (rabbit polyclonal,
Abcam
ab18251
, Cambridge, UK). Alternatively, at
treatment cessation
, cells were fixed with ice-cold methanol at −20
(103)
Cep215 is essential for morphological differentiation of astrocytes
Scientific reports
October 12, 2020
Kang D, Shin W, Yoo H, Kim S, Lee S, Rhee K.
Cell Signaling
), Sox9 (
AB5535
;
Millipore
),
Nfia
(
HPA006111
;
Sigma
), Flag (
F3165
;
Sigma-Aldrich
) and α-tubulin (
ab18251
;
Abcam
) were commercially purchased. The
goat
γ-tubulin (
sc-7396
;
Santa Cruz
) and rabbit γ-tubulin (
ab11317
;
(104)
Cytotoxic activity of bromodomain inhibitor NVS-CECR2-1 on human cancer cells
Scientific reports
October 1, 2020
Park SG, Lee D, Seo HR, Lee SA, Kwon J.
anti-
BRG1
(
Santa Cruz Biotechnology
,
sc-17796
), anti-c-Myc (BIOMOL, SA-294), anti-α-Tubulin (
Abcam
,
ab18251
), anti-GAPDH (Abfrontier, LF-PA0212),
anti-Lamin A
/C (Cell Signaling Technology, 2032), anti-
PARP1
(
Santa Cruz Biotechnology
(105)
Hypoxia regulates the degradation of non-nuclear organelles during lens differentiation through activation of HIF1a
Experimental eye research
September 1, 2020
Brennan L, Disatham J, Kantorow M.
determined by stripping blots and probing for standard equal loading marker α-Tubulin (Catalog no.
ab18251
,
Abcam
). Analysis of
BNIP3L
and
HIF1a
gene expression
in chick lens
organ culture
BNIP3L
and
HIF1a
(106)
Vascular Shutdown by Photodynamic Therapy Using Talaporfin Sodium
Cancers (Basel)
August 21, 2020
Taketo Suzuki, Mamoru Tanaka, Makiko Sasaki, Hiroshi Ichikawa, Hirotada Nishie, Hiromi Kataoka
Thereafter, the cells were
fixed with paraformaldehyde
and incubated with primary antibodies against tubulin (
ab18251
,
Abcam
, Cambridge, MA, USA) or F-actin (
bs-1571R
,
Bioss
, Woburn, MA, USA). Alexa Fluor 488-conjugated
(107)
Targeting CLK3 inhibits the progression of cholangiocarcinoma by reprogramming nucleotide metabolism
The Journal of experimental medicine
August 3, 2020
Zhou Q, Lin M, Feng X, Ma F, Zhu Y, Liu X, Qu C, Sui H, Sun B, Zhu A,
et al
.
CLK4 (
Abcam
, #
ab67936
),
PFAS
, (
Abcam
; #
ab251740
), phosphoserine (
Abcam
; #
ab9332
), Tubulin (
Abcam
; #
ab18251
),
USP13
antibody (
Bethyl Laboratories
; #
A302-762A
), phosphothreonine (
Abcam
; #
ab9337
), phosphotyrosine
(108)
Expression of the Long Noncoding RNA DINO in Human Papillomavirus-Positive Cervical Cancer Cells Reactivates the Dormant TP53 Tumor Suppressor through ATM/CHK2 Signaling
mBio
June 30, 2020
Surendra Sharma, Karl Munger
phospho-ATM (Ser1981) (5833; CST), phospho-
Chk2
(Thr68) (
2197
;
CST
) and MDM2 (OP46; Millipore), and ␣-tubulin (
ab18251
;
Abcam
) specific antibodies at the dilutions recommended by the manufacturers. Secondary antibodies were
(109)
ROS-Mediated Apoptotic Cell Death of Human Colon Cancer LoVo Cells by Milk δ-Valerobetaine
Scientific reports
June 2, 2020
D'Onofrio N, Cacciola NA, Martino E, Borrelli F, Fiorino F, Lombardi A, Neglia G, Balestrieri ML, Campanile G.
(
ab139484
), Rapamycin (
ab139484
); anti-
SIRT6
(
ab191385
) and anti-β-actin (
ab8227
), anti-alpha tubulin (
ab18251
),
Phalloidin-iFluor 488 Reagent
(
ab176753
) were from
Abcam
(Cambridge CB2 0AX UK); anti-Bax (5023),
(110)
KDM6A-Mediated Expression of the Long Noncoding RNA DINO Causes TP53 Tumor Suppressor Stabilization in Human Papillomavirus 16 E7-Expressing Cells
Journal of Virology
June 1, 2020
Surendra Sharma, Karl Munger
(
ab36938
,
1:300
;
Abcam
),
CDKN1A
(
ab109520
,
1:1,000
;
Abcam
), p14 (
sc-8613
,
1:200
;
Santa Cruz
), ␣-tubulin (
ab18251
,
1:1,000
;
Abcam
), and actin (Ab-1501,
1:1,000
; Millipore).
Membranes
were rinsed in TNET and incubated
(111)
Beta Human Papillomavirus 8E6 Attenuates LATS Phosphorylation after Failed Cytokinesis
Journal of Virology
June 1, 2020
Dalton Dacus, Celeste Cotton, Tristan X. McCallister, Nicholas A. Wallace
Technologies; product no.
4912S
), Ki67 (
Abcam
; catalog no.
ab15580
), alpha-tubulin (
Abcam
; catalog no.
ab18251
), and ␣-tubulin (
Cell Signaling Technologies
; product no.
3873S
). The cells were washed and stained
(112)
Mitotic checkpoint protein Mad1 is required for early Nup153 recruitment to chromatin and nuclear envelope integrity
Journal of Cell Science
May 21, 2020
Ikram Mossaid, Guillaume Chatel, Valérie Martinelli, Marcela Vaz, Birthe Fahrenkrog
sc-67338
; IF
1:100
); rabbit anti-Mad2 (Covance; PRB-452C; IF
1:200
); rabbit anti-α-tubulin (
Abcam
;
ab18251
; WB
1:4000
); rabbit anti-actin (
Sigma-Aldrich
;
A2066
; WB
1:1000
); rabbit anti-Nup88 (
BD Biosciences
;
611896
;
(113)
Tau affects P53 function and cell fate during the DNA damage response
Communications biology
May 19, 2020
Sola M, Magrin C, Pedrioli G, Pinton S, Salvadè A, Papin S, Paganetti P.
(#
86934
,
Cell Signaling
), 0.05 μg/mL clAsp175-Caspase3 (#
9661
,
Cell Signaling
), 0.5 μg/mL alpha-tubulin (
ab18251
,
Abcam
). Detection of endogenous
Tau
entailed the testing of a number of commercial antibodies so to
(114)
EDEM1 Drives Misfolded Protein Degradation via ERAD and Exploits ER-Phagy as Back-Up Mechanism When ERAD Is Impaired
International Journal of Molecular Sciences
May 14, 2020
Marioara Chiritoiu, Gabriela N. Chiritoiu, Cristian V. A. Munteanu, Florin Pastrama, N. Erwin Ivessa, Stefana M. Petrescu
rabbit α-calnexin (
ab22595
), rabbit α-
OS-9
(
ab19853
), rabbit α-
XTP3-B
(
ab181166
), rabbit α-tubulin (
ab18251
), and rabbit α-Grp94 (
ab3674
) were from
Abcam
(Cambridge, UK);
mouse
α-LC3 (0231-Nanotools, Teningen,
(115)
Asymmetric clustering of centrosomes defines the early evolution of tetraploid cells
eLife
April 29, 2020
Nicolaas C Baudoin, Joshua M Nicholson, Kimberly Soto, Olga Martin, Jing Chen, Daniela Cimini
monoclonal),
Abcam
,Cat#
ab195047
RRID:AB_2832993,IF(
1:100
) Antibody,anti-α-tubulin (Rabbit polyclonal),
Abcam
,Cat#:
ab18251
RRID:AB_2210057,IF(
1:250
) Other,DAPI stain,
Invitrogen
,
D1306
,(300 nM) Recombinant DNA reagent,
GFP
-CETN2
(116)
Staufen1 localizes to the mitotic spindle and controls the localization of RNA populations to the spindle
Journal of Cell Science
April 1, 2020
Sami Hassine, Florence Bonnet-Magnaval, Louis Philip Benoit Bouvrette, Bellastrid Doran, Mehdi Ghram, Mathieu Bouthillette, Eric Lecuyer, Luc DesGroseillers
antibodies were purchased from
SigmaAldrich
. Anti-aurora A (
30925
, batch number 2;
1:2000
), anti-α-tubulin (
ab18251
, batch number GR201260-1;
1:40,000
for IF) and anti-
histone H3
(
ab1791
, batch number GR204148-1;
1:3000
)
(117)
Tuberculosis-associated IFN-I induces Siglec-1 on tunneling nanotubes and favors HIV-1 spread in macrophages
eLife
March 30, 2020
Dupont M, Souriant S, Balboa L, Vu Manh TP, Pingris K, Rousset S, Cougoule C, Rombouts Y, Poincloux R, Ben Neji M,
et al
.
20‑33),
Sigma‑Aldrich
,Cat#
A5060
RRID:AB_476738,WB (
1:100
) Antibody,Rabbit polyclonal anti-a-tubulin,
Abcam
,Cat#
ab18251
RRID:AB_2210057,IF (5 µg/mL) Antibody,
Mouse
monoclonal anti-Siglec-1 (
clone
hsn 7D2),Novus Biologicals,Cat#
(118)
A nuclear localization signal is required for the nuclear translocation of Fign and its microtubule‑severing function
Molecular Medicine Reports
March 26, 2020
Jiong Li, Fengming Wu, Longfei Cheng, Jiaqi Zhang, Caihui Cha, Li Chen, Taoshan Feng, Jifeng Zhang, Guoqing Guo
incubated overnight at 4°C with the following primary antibodies: Anti-α-tubulin (
1:2,000
; cat. no.
ab18251
; rabbit polyclonal;
Abcam
); anti-tyrosinated tubulin (
1:2,000
; cat. no.
ab6160
;
rat
monoclonal;
Abcam
);
(119)
An improved method for culturing myotubes on laminins for the robust clustering of postsynaptic machinery
Scientific reports
March 11, 2020
Pęziński M, Daszczuk P, Pradhan BS, Lochmüller H, Prószyński TJ.
Proteintech
), anti-rapsyn antibody (catalog no.
ab156002
,
Abcam
) or anti-tubulin antibody (catalog no.
ab18251
,
Abcam
) in 5% milk in TBST. After washing with TBST buffer, the
membranes
were incubated with appropriate
(120)
Asymmetrically Segregated Mitochondria Provide Cellular Memory of Hematopoietic Stem Cell Replicative History and Drive HSC Attrition
Cell stem cell
March 5, 2020
Hinge A, He J, Bartram J, Javier J, Xu J, Fjellman E, Sesaki H, Li T, Yu J, Wunderlich M,
et al
.
Microscopy
/ Imaging
tomm20
,
Abcam
,
ab186734
drp-1,BD transduction laboratories,611112 alpha tubulin,
Abcam
,
ab18251
anti-rabbit – AF488,
Invitrogen
,
A21206
anti-rabbit AF546,
Life technologies
,
A11010
anti-
mouse
AF555,
Life technologies
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Reviews
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(1)
3/13/2017
Antibody Against Alpha Tubulin For Immunofluorescence
Adam Kramer
This antibody is great to image the microtubule cytoskeleton in cultured mammalian cells
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Anti-alpha Tubulin antibody - Microtubule Marker from Abcam
Supplier Page
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