Antibody Against Alpha Tubulin For Immunofluorescence

March 13, 2017

Physiology and Biophysics
Albert Einstein College of Medicine
Graduate student

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Company:

Abcam

Product Name:

Anti-alpha Tubulin antibody

Catalog Number:

ab18251

This antibody is great to image the microtubule cytoskeleton in cultured mammalian cells

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Fixed HUVEC cells

Primary Incubation

1:400 overnight

Blocking Agent

5% normal goat serum in PBS and 0.1% triton

Secondary Incubation

1:500

Tertiary Incubation

N/A

Detection

EVOS FL Cell Imaging System

Results Summary

Crisp images of the MT cytoskeleton were achieved using this antibody

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Crisp staining

The Bad

Overnight incubation

The Bottom Line

Great antibody to image microtubules in fixed cells

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