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Anti-alpha Tubulin antibody - Microtubule Marker
Anti-alpha Tubulin antibody - Microtubule Marker from Abcam
(1)
Write a review
Citations:
(308)
Supplier Page
Supplier Page from
Abcam for
Anti-alpha Tubulin antibody - Microtubule Marker
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-alpha Tubulin antibody - Microtubule Marker
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab18251
Antibody Type
Primary
Quantity
50 UG
Isotype
IgG
Host
Rabbit
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western blot
Clonality
Polyclonal
Conjugate/Tag
Unconjugated
Concentration
0.3 mg/ml
Gene Name
TUBA4A
Reactivity
Human, Mouse, Rat
Target
alpha Tubulin
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (308)
(81)
Dimerisation of the PICTS complex via LC8/Cut-up drives co-transcriptional transposon silencing in Drosophila
eLife
February 4, 2021
Evelyn L Eastwood, Kayla A Jara, Susanne Bornelöv, Marzia Munafò, Vasileios Frantzis, Emma Kneuss, Elisar J Barbar, Benjamin Czech, Gregory J Hannon
,
Cell Signaling Technology
,"Cat#
3724
, RRID:AB_1549585","WB(
1:2,500
)" Antibody,
Anti-tubulin
(Rabbit polyclonal),
Abcam
,"Cat#
ab18251
, RRID:AB_2210057","WB(
1:2,500
)" Antibody,Anti-tubulin (Mouse monoclonal),
Abcam
,"Cat#
ab44928
, RRID:AB_2241150","WB(
1:2,500
)" Antibody,Anti-His3
(82)
Time-of-day specificity of anticancer drugs may be mediated by circadian regulation of the cell cycle
Science advances
February 1, 2021
Lee Y, Fong SY, Shon J, Zhang SL, Brooks R, Lahens NF, Chen D, Dang CV, Field JM, Sehgal A.
Anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase) (
sc25778
,
Santa Cruz Biotechnology
), anti-tubulin (
ab18251
,
Abcam
), and anti–β-actin (
4967S
,
Cell Signaling
) were used as loading control antibodies.
Immunoprecipitation
For
(83)
An ATM-Chk2-INCENP pathway activates the abscission checkpoint
The Journal of cell biology
February 1, 2021
Petsalaki E, Zachos G.
Santa Cruz Biotechnology
. Rabbit polyclonal anti-Survivin (ab469; used in
immunofluorescence
), anti–α-tubulin (
ab18251
; used in
immunofluorescence
), anti-INCENP (
ab12183
), anti-
Chk2
-pThr383 (
ab59408
), and anti–Aurora B
(84)
Reticulon-3 Promotes Endosome Maturation at ER Membrane Contact Sites
Developmental cell
January 11, 2021
Wu H, Voeltz GK.
antibody (
MilliporeSigma
,
G9545
) was used at
1: 100000
for immunoblotting
. Rabbit
Tubulin antibody
(
Abcam
,
ab18251
) was used at
1: 100000
immunoblotting
. Rabbit
Rab9a
antibody (
CST
,
5133
) was used at
1:1000
for immunoblotting
.
(85)
UNC-45A breaks the microtubule lattice independently of its effects on non-muscle myosin II
Journal of cell science
January 8, 2021
Habicht J, Mooneyham A, Hoshino A, Shetty M, Zhang X, Emmings E, Yang Q, Coombes C, Gardner MK, Bazzaro M.
anti-α-tubulin (
Sigma-Aldrich
,
T6074
;
1:1000
, WB;
1:2000
, IF), rabbit polyclonal anti-αtubulin (
Abcam
,
ab18251
;
1:1000
, WB),
mouse
monoclonal antidetyrosinated-α-tubulin (
EMD Millipore
,
AB3201
;
1:1000
, WB), rabbit monoclonal
(86)
Junctional ER Organization Affects Mechanotransduction at Cadherin-Mediated Adhesions
Frontiers in cell and developmental biology
January 1, 2021
Joy-Immediato M, Ramirez MJ, Cerda M, Toyama Y, Ravasio A, Kanchanawong P, Bertocchi C.
(
Abcam
#
ab11512
)
1:1,600
; rabbit anti-β-catenin (
Abcam
#
ab16051
)
1:400
; rabbit anti-tubulin (
Abcam
#
ab18251
)
1:500
;
rat
anti-tubulin (
GeneTex
#
GTX76511
)
1:500
;
mouse
anti-
PTP1B
(
Abcam
#
ab124375
)
1:400
. After
(87)
Proteome Profiling of RNF213 Depleted Cells Reveals Nitric Oxide Regulator DDAH1 Antilisterial Activity
Frontiers in cellular and infection microbiology
January 1, 2021
Martina L, Asselman C, Thery F, Boucher K, Delhaye L, Maia TM, Dermaut B, Eyckerman S, Impens F.
Santa Cruz Biotechnology
), rabbit polyclonal anti-RNF213 (
HPA003347
,
Merck
), rabbit polyclonal anti-tubulin-α antibody (#
ab18251
,
Abcam
),
mouse
monoclonal anti-FLAG-tag (M2, #
F3165
,
Merck
). Aforementioned primary antibodies were
(88)
The Expandables: Cracking the Staphylococcal Cell Wall for Expansion Microscopy
Frontiers in cellular and infection microbiology
January 1, 2021
Kunz TC, Rühling M, Moldovan A, Paprotka K, Kozjak-Pavlovic V, Rudel T, Fraunholz M.
and
OriGene
,
SP3005P
, dilution
1:100
), anti-
LC3B
(
Cell Signaling
,
2775
, dilution
1:100
), anti-tubulin (
Abcam
,
ab18251
, dilution
1:100
and
Sigma Aldrich
,
T8328
, dilution
1:100
), antilipoteichoic acid (OriGene, BP984, dilution
(89)
PARP1 Regulates the Biogenesis and Activity of Telomerase Complex Through Modification of H/ACA-Proteins
Frontiers in cell and developmental biology
January 1, 2021
Savelyev NV, Shepelev NM, Lavrik OI, Rubtsova MP, Dontsova OA.
11711-1-AP
(
Proteintech
), PAR 4336-BPC (Trevigen),
GAPDH
39-8600
(
Thermo Fisher Scientific
), α-tubulin
ab18251
(
Abcam
),
horseradish
peroxidase-conjugated
donkey
anti-
mouse
A16011
(
Thermo Fisher Scientific
), and
(90)
Chaperone-Assisted Mitotic Actin Remodeling by BAG3 and HSPB8 Involves the Deacetylase HDAC6 and Its Substrate Cortactin
International Journal of Molecular Sciences
December 25, 2020
Carole Luthold, Alice-Anaïs Varlet, Herman Lambert, François Bordeleau, Josée N. Lavoie
mouse
(
T5168
,
1:10000
;
Sigma-Aldrich
); anti-cortactin (
ab33333
,
1:1000
) and anti-α-tubulin rabbit (
ab18251
,
1:1000
;
Abcam
); anti-acetyl-cortactin K309 (ABT1378,
1:1000
); anti-cyclin B1 (ac-245,
1:1000
) and anti-GAPDH
(91)
U2AF65-Dependent SF3B1 Function in SMN Alternative Splicing
Cells
December 9, 2020
Namjeong Choi, Yongchao Liu, Jagyeong Oh, Jiyeon Ha, Xuexiu Zheng, Haihong Shen
to polyvinylidene fluoride (PVDF)
membranes
. Anti-
SF3B1
(
Abcam
,
ab172634
) and anti-tubulin (
Abcam
,
ab18251
) antibodies were used
for immunoblotting
analysis.
RNA pull-down
/
immunoblotting
analysis was performed
(92)
Drosophila
MICOS knockdown impairs mitochondrial structure and function and promotes mitophagy in muscle tissue
Biology Open
December 3, 2020
Li-jie Wang, Tian Hsu, Hsiang-ling Lin, Chi-yu Fu
analysis. Mouse anti-Flag M2 (1 μg/ml,
Sigma-Aldrich
F3165
), rabbit anti-alpha tubulin (10000x,
Abcam
ab18251
), anti-mouse IgG-HRP (2000×,
Invitrogen
62-6520
), and anti-rabbit IgG-HRP (5000x,
Abcam
ab97051
) were
(93)
The scaffold protein IQGAP1 is crucial for extravasation and metastasis
Scientific Reports
December 1, 2020
Jess D. Hebert, Chenxi Tian, John M. Lamar, Steffen Rickelt, Genevieve Abbruzzese, Xiaotie Liu, Richard O. Hynes
TRITC-phalloidin (
1:1000
, Sigma) was used to detect actin, along with rabbit anti-α-tubulin (
1:500
,
ab18251
,
Abcam
). To label cover slips with fluorescent gelatin, 18 mm circular No. 2 cover glass (VWR) was washed
(94)
Follicular extracellular vesicles enhance meiotic resumption of domestic cat vitrified oocytes
Scientific Reports
December 1, 2020
Marcia de Almeida Monteiro Melo Ferraz, Mayako Fujihara, Jennifer Beth Nagashima, Michael James Noonan, Miho Inoue-Murayama, Nucharin Songsasen
in PBS with 5% normal
goat
serum and 0.5% Triton X before
incubation
with rabbit anti-alpha tubulin (
ab18251
,
Abcam
) at Scientific Reports | (2020) 10:8619 | https://doi.org/10.1038/s41598-020-65497-w 10 www.nature.com/scientificreports/ www.nature.com/scientificreports Figure
(95)
Mms19 promotes spindle microtubule assembly in Drosophila neural stem cells
PLOS Genetics
November 19, 2020
Rohan Chippalkatti, Boris Egger, Beat Suter
antibodies,,, Antibody,Manufacturer,Catalog number,Dilution
Rat
anti-Miranda,
Abcam
,
Ab197788
,
1:250
Rabbit anti-alpha Tubulin,
Abcam
,
Ab18251
,
1:500
Mouse
anti-alpha Tubulin,
Sigma
,
T6199
,
1:500
Rabbit anti-GFP,Immunokontakt,210-PS-1GFP,
1:500
Rabbit
(96)
BLOS1 mediates kinesin switch during endosomal recycling of LDL receptor
eLife
November 12, 2020
Zhang C, Hao C, Shui G, Li W.
Cat#
sc-7392
, 1/100;
Abcam
, Cat#
ab9134
, 1/1000) and anti-α-Tubulin (
Abcam
, Cat#
ab7291
, 1/500;
Abcam
, Cat#
ab18251
, 1/1000). Coverslips were incubated with primary antibodies diluted in blocking buffer overnight at
(97)
Centrosome and ciliary abnormalities in fetal akinesia deformation sequence human fibroblasts
Scientific reports
November 9, 2020
Jühlen R, Martinelli V, Vinci C, Breckpot J, Fahrenkrog B.
α-tubulin (
1:1000
), rabbit anti-detyrosinated α -tubulin (
1:1000
), and rabbit anti-α-tubulin (
1:4000
;
ab18251
,
Abcam
). Secondary antibodies were either alkaline phosphatase-coupled IgG antibodies (
1:10
.000; Sigma/Aldrich)
(98)
Dominant-negative NFKBIA mutation promotes IL-1β production causing hepatic disease with severe immunodeficiency
The Journal of clinical investigation
November 2, 2020
Tan EE, Hopkins RA, Lim CK, Jamuar SS, Ong C, Thoon KC, Koh MJ, Shin EM, Lian DW, Weerasooriya M,
et al
.
in TBS–Tween-20. Blocking then occurred again as previously stated, followed by
incubation
with anti–α-tubulin (
ab18251
,
Abcam
) or anti–
GAPDH
-HRP (
ab9385
,
Abcam
) for 16 hours at 4°C. Detection of bands occurred as previously
(99)
Phosphatidylinositol-3-OH kinase signalling is spatially organized at endosomal compartments by microtubule-associated protein 4
Nature cell biology
November 1, 2020
Thapa N, Chen M, Horn HT, Choi S, Wen T, Anderson RA.
phospho-Erk1/2 (#
4370
,
Cell Signaling
), rabbit anti-GAPDH (#
2118
,
Cell Signaling
), rabbit anti-tubulin (#
ab18251
,
Abcam
),
rat
anti-tubulin (#
ab6160
,
Abcam
), rabbit anti-tubulin (#
ab6046
,
Abcam
),
mouse
anti-clathrin
(100)
Opposite Roles of Tra2β and SRSF9 in the v10 Exon Splicing of CD44
Cancers (Basel)
October 30, 2020
Jagyeong Oh, Yongchao Liu, Namjeong Choi, Jiyeon Ha, Davide Pradella, Claudia Ghigna, Xuexiu Zheng, Haihong Shen
anti-Tra2β (
Abcam
,
ab31353
, Cambridge, UK), anti-SRSF9 (Santa Cruz,
sc-134036
), and anti-α-tubulin (
Abcam
,
ab18251
) antibodies, respectively. 2.5. RNA-Binding Motifs Prediction Tra2β putative RNA-binding motifs prediction
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Reviews
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(1)
3/13/2017
Antibody Against Alpha Tubulin For Immunofluorescence
Adam Kramer
This antibody is great to image the microtubule cytoskeleton in cultured mammalian cells
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Anti-alpha Tubulin antibody - Microtubule Marker from Abcam
Supplier Page
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