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Anti-alpha Tubulin antibody - Microtubule Marker
Anti-alpha Tubulin antibody - Microtubule Marker from Abcam
(1)
Write a review
Citations:
(308)
Supplier Page
Supplier Page from
Abcam for
Anti-alpha Tubulin antibody - Microtubule Marker
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-alpha Tubulin antibody - Microtubule Marker
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab18251
Antibody Type
Primary
Quantity
50 UG
Isotype
IgG
Host
Rabbit
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western blot
Clonality
Polyclonal
Conjugate/Tag
Unconjugated
Concentration
0.3 mg/ml
Gene Name
TUBA4A
Reactivity
Human, Mouse, Rat
Target
alpha Tubulin
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (308)
(61)
Sub-centrosomal mapping identifies augmin-γTuRC as part of a centriole-stabilizing scaffold
Nature communications
October 15, 2021
Schweizer N, Haren L, Dutto I, Viais R, Lacasa C, Merdes A, Lüders J.
1:500
or
1:250
(ExM)), rabbit anti-γ-tubulin R7586 (
1:1000
or
1:250
(U-ExM)), rabbit anti-α-tubulin (
ab18251
,
Abcam
;
1:300
or
1:500
(ExM and U-ExM)),
mouse
anti-acetylated α-tubulin (
clone
6-11B-1, Merck; IF,
(62)
Ring finger protein 213 assembles into a sensor for ISGylated proteins with antimicrobial activity
Nature communications
October 1, 2021
Thery F, Martina L, Asselman C, Zhang Y, Vessely M, Repo H, Sedeyn K, Moschonas GD, Bredow C, Teo QW,
et al
.
anti-
LC3B
(
1:1,000
; #
PA146286
,
Thermo Fisher Scientific
), rabbit polyclonal anti-tubulin-α (
1:1,000
; #
ab18251
,
Abcam
), anti-p24 GAG (
1:1,000
; #
ab9071
,
Abcam
), rabbit polyclonal anti-PLIN1 (
1:1,000
; #
ab3526
,
Abcam
),
(63)
ERα is an RNA-binding protein sustaining tumor cell survival and drug resistance
Cell
September 30, 2021
Xu Y, Huangyang P, Wang Y, Xue L, Devericks E, Nguyen HG, Yu X, Oses-Prieto JA, Burlingame AL, Miglani S,
et al
.
RRID:AB_1031062 "Anti-Lamin A/C,
mouse
",
Santa Cruz
,Cat#
sc-7292
; RRID:AB_627875 "Anti-α-tubulin,
mouse
",
Abcam
,Cat#
ab18251
; RRID:AB_2210057 "Anti-
SMC2
, rabbit",
Cell Signaling Technology
,Cat#
5329S
; RRID:AB_10693789 "
Anti-PABP1
,
(64)
Drebrin Regulates Acetylcholine Receptor Clustering and Organization of Microtubules at the Postsynaptic Machinery
International journal of molecular sciences
August 30, 2021
Alvarez-Suarez P, Nowak N, Protasiuk-Filipunas A, Yamazaki H, Prószyński TJ, Gawor M.
(
838301
,
BioLegend
), anti-rapsyn (
ab156002
,
Abcam
), anti-GAPDH (
sc-25778
,
Santa Cruz
) and anti-tubulin (
ab18251
,
Abcam
). After washing with TBST, the
membranes
were incubated with secondary antibodies conjugated
(65)
Reverse Plasticity Underlies Rapid Evolution by Clonal Selection within Populations of Fibroblasts Propagated on a Novel Soft Substrate
Molecular Biology and Evolution
July 29, 2021
Purboja Purkayastha, Kavya Pendyala, Ayush S Saxena, Hesamedin Hakimjavadi, Srikar Chamala, Purushottam Dixit, Charles F Baer, Tanmay P Lele
at room temperature.
Microtubules
were labeled using rabbit polyclonal antialpha tubulin antibody (
ab18251
;
Abcam
; dilution
1:600
); and
YAP
was labeled using
mouse
monoclonal anti-YAP antibody (
sc-271134
;
Santa Cruz Biotechnology
(66)
Affinity Proteomics and Deglycoproteomics Uncover Novel EDEM2 Endogenous Substrates and an Integrative ERAD Network
Molecular & Cellular Proteomics
July 29, 2021
Cristian V.A. Munteanu, Gabriela N. Chirițoiu, Marioara Chirițoiu, Simona Ghenea, Andrei-Jose Petrescu, Ştefana M. Petrescu
used for WB
detection were HA (
11867431001
) from
Roche
, CANX (
ab22595
),
ERLEC1
(
ab181166
), and tubulin (
ab18251
) from
Abcam
, actin (
612657
) from
BD Biosciences
,
DERL1
(
D4443
) and
EDEM2
(
E1782
) from
Sigma
,
PDIA4
(
sc-135901
),
(67)
Defining the Basis of Cyanine Phototruncation Enables a New Approach to Single-Molecule Localization Microscopy
ACS Central Science
July 28, 2021
Siddharth S. Matikonda, Dominic A. Helmerich, Mara Meub, Gerti Beliu, Philip Kollmannsberger, Alexander Greer, Markus Sauer, Martin J. Schnermann
Subsequently,
microtubule
samples were incubated with 2 ng/μL rabbit anti-α-tubulin primary antibody (
Abcam
, #
ab18251
) in blocking buffer for 1 h. After primary antibody
incubation
, cells were rinsed with PBS (Sigma-Aldrich,
(68)
Chromosome oscillation promotes Aurora A-dependent Hec1 phosphorylation and mitotic fidelity
The Journal of cell biology
July 5, 2021
Iemura K, Natsume T, Maehara K, Kanemaki MT, Tanaka K.
A301-080A
) WB
1:2,000
; anti-
GFP
(
Life Technologies
;
A11122
) IF
1:2,000
; and anti–α-tubulin (
Abcam
;
ab18251
) IF
1:1,000
.
Anti-centromere protein
human
autoimmune serum (
Antibodies Inc
.;
15–234
) was used for IF
(69)
SeqStain is an efficient method for multiplexed, spatialomic profiling of human and murine tissues
Cell Rep Methods
June 21, 2021
Rajagopalan A, Venkatesh I, Aslam R, Kirchenbuechler D, Khanna S, Cimbaluk D, Kordower JH, Gupta V.
RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,,
CD11b
(
clone
M1/70),
Biolegend
,Cat#
101248
; RRID: AB_2561479 α-Tubulin,
Abcam
,Cat#
ab18251
: RRID: AB_2210057 Mouse CD45 (
clone
30-F11),
Biolegend
,Cat#
103164
; RRID: AB_2819790 Vinculin (
clone
(70)
Modulation of mitochondrial nucleoid structure during aging and by mtDNA content in
Drosophila
Biology Open
June 15, 2021
Li-jie Wang, Tian Hsu, Hsiang-ling Lin, Chi-yu Fu
Abcam
ab14748
), mouse anti-Flag M2 (1 μg/ml,
Sigma
F3165
), rabbit anti-alpha tubulin (
1:10,000
,
Abcam
ab18251
), anti-mouse IgG-HRP (
1:2000
,
Invitrogen
62-6520
), and anti-rabbit IgG-HRP (
1:5000
,
Abcam
ab97051
) antibodies
(71)
Engineering of CD19 Antibodies: A CD19-TRAIL Fusion Construct Specifically Induces Apoptosis in B-Cell Precursor Acute Lymphoblastic Leukemia (BCP-ALL) Cells In Vivo
Journal of Clinical Medicine
June 15, 2021
Dorothee Winterberg et al.
analyzed using an apoptosis antibody sampler kit (#
9915
,
Cell Signaling Technology
, Danvers, MA, USA). Tubulin (#
ab18251
,
Abcam
, Cambridge, UK) was detected as a loading control. 2.9.
Animal Experiments
Xenograft experiments
(72)
Engineering the Escherichia coli Nitroreductase NfsA to Create a Flexible Enzyme-Prodrug Activation System
Frontiers in Pharmacology
June 7, 2021
Abigail V. Sharrock, Sarah P. McManaway, Michelle H. Rich, Jeff S. Mumm, Ian F. Hermans, Moana Tercel, Frederik B. Pruijn, David F. Ackerley
University of Birmingham) diluted to
1:5,000
in TBS + 0.1% (v/v) Tween-20, or rabbit anti-α-tubulin (
Abcam
;
ab18251
) diluted to
1:5,000
. Following
incubation
, the
membrane
was decanted and washed three times in TBS +
(73)
Three-dimensional residual channel attention networks denoise and sharpen fluorescence microscopy image volumes
Nature Methods
June 1, 2021
Jiji Chen et al.
Scientific,
37525
) and incubated overnight with
1:500
dilution of primary rabbit anti-alpha tubulin (
Abcam
,
ab18251
) and
mouse
anti-
nuclear pore complex
(
Abcam
,
ab24609
) antibodies in 1x Blocker BSA at 4°C. Samples were
(74)
Emodin Improves Glucose and Lipid Metabolism Disorders in Obese Mice via Activating Brown Adipose Tissue and Inducing Browning of White Adipose Tissue
Front Endocrinol (Lausanne)
May 10, 2021
Long Cheng, Shuofeng Zhang, Fei Shang, Yibo Ning, Zhiqi Huang, Runcheng He, Jianning Sun, Shifen Dong
membranes
. PVDF
membranes
containing protein were incubated with specific anti-alpha tubulin antibody (
1:5000
,
ab18251
,
Abcam
), anti-
UCP1
antibody (
1:1000
,
ab10983
, Abcam), anti-Prohibitin antibody (
1:10000
,
ab75766
, Abcam),
(75)
SUMOylation of α-tubulin is a novel modification regulating microtubule dynamics
Journal of Molecular Cell Biology
May 7, 2021
Wenfeng Feng, Rong Liu, Xuan Xie, Lei Diao, Nannan Gao, Jinke Cheng, Xu Zhang, Yong Li, Lan Bao
4940S
),
SUMO2
/3 (
1:1000
;
CST
,
4971S
), α-tubulin (
1:10000
; Sigma,
T9026
), α-tubulin (
1:10000
;
Abcam
,
ab18251
), Flag (
1:10000
;
Sigma
,
F7425
), β-actin (
1:50000
;
Chemicon
,
MAB1501
), and GAPDH (
1:10000
;
Abcam
,
ab8245
).
(76)
Channel nuclear pore complex subunits are required for transposon silencing in Drosophila
eLife
April 15, 2021
Munafò M, Lawless VR, Passera A, MacMillan S, Bornelöv S, Haussmann IU, Soller M, Hannon GJ, Czech B.
polyclonal),DOI:10.1038/emboj.2011.308,,WB(
1:1000
) Antibody,
Anti-tubulin
(Rabbit polyclonal),
Abcam
,"Cat#
ab18251
, RRID:AB_2210057",WB(
1:5000
) Antibody,Anti-HA (Rabbit monoclonal),
Cell Signaling Technology
,"Cat#
3724
,
(77)
Identification of BAG2 and Cathepsin D as Plasma Biomarkers for Parkinson's Disease
Clinical and translational science
March 1, 2021
Kang J, Kim JW, Heo H, Lee J, Park KY, Yoon JH, Chang J.
#
ab25630
),
LAMP2
(
Proteintech
Cat #
66301‐1‐Ig
),
WDFY3
(
Bethyl
Cat #
A301‐869A
), and α‐Tubulin (
Abcam
Cat #
ab18251
) were purchased. Immunoreactive proteins were detected with a ChemiDoc
Gel Imaging
System (Bio‐rad).
(78)
CHIP and BAP1 Act in Concert to Regulate INO80 Ubiquitination and Stability for DNA Replication
Molecules and Cells
February 28, 2021
Hye-Ran Seo, Daun Jeong, Sunmi Lee, Han-Sae Lee, Shin-Ai Lee, Sang Won Kang, Jongbum Kwon
primary antibodies were as follows: anti-Actin (
sc-8432
;
Santa Cruz Biotechnology
, USA), anti-α-tubulin (
ab18251
;
Abcam
, UK), anti-
BAP1
(
sc-28383
;
Santa Cruz Biotechnology
), anti-CHIP (
2080
;
Cell Signaling Technology
,
(79)
Imd pathway-specific immune assays reveal NF-κB stimulation by viral RNA PAMPs in Aedes aegypti Aag2 cells
PLOS Neglected Tropical Diseases
February 16, 2021
Tiffany A. Russell, Andalus Ayaz, Andrew D. Davidson, Ana Fernandez-Sesma, Kevin Maringer
anti-FLAG ‘M2’ (
Sigma-Aldrich
;
F3165
) at
1:5,000
dilution, rabbit anti-tubulin (alpha) ‘EP1332Y’ (
Abcam
;
ab18251
) at
1:2,000
dilution.
Membranes
were then incubated with IRDye 800CW
goat anti-mouse IgG (H+L
) or IRDye
(80)
Cytoskeletal Organization Correlates to Motility and Invasiveness of Malignant Mesothelioma Cells
Cancers (Basel)
February 8, 2021
Maureen Keller, Katarina Reis, Anders Hjerpe, Katalin Dobra, Pontus Aspenström
anti-phosphotyrosine (PY99,
SC-7020
) (
Santa Cruz
, Santa Cruz, CA, USA); rabbit polyclonal anti–α-tubulin (
ab18251
;
Abcam
, Cambridge, UK);
mouse
monoclonal antinestin (#
33475
;
Cell Signaling
, Danvers, MA, USA); rabbit
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Reviews
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(1)
3/13/2017
Antibody Against Alpha Tubulin For Immunofluorescence
Adam Kramer
This antibody is great to image the microtubule cytoskeleton in cultured mammalian cells
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Anti-alpha Tubulin antibody - Microtubule Marker from Abcam
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