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Anti-alpha Tubulin antibody - Microtubule Marker
Anti-alpha Tubulin antibody - Microtubule Marker from Abcam
(1)
Write a review
Citations:
(308)
Supplier Page
Supplier Page from
Abcam for
Anti-alpha Tubulin antibody - Microtubule Marker
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-alpha Tubulin antibody - Microtubule Marker
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab18251
Antibody Type
Primary
Quantity
50 UG
Isotype
IgG
Host
Rabbit
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western blot
Clonality
Polyclonal
Conjugate/Tag
Unconjugated
Concentration
0.3 mg/ml
Gene Name
TUBA4A
Reactivity
Human, Mouse, Rat
Target
alpha Tubulin
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (308)
(21)
Microtubule nucleation and γTuRC centrosome localization in interphase cells require ch-TOG
Nature Communications
January 26, 2023
Aamir Ali, Chithran Vineethakumari, Cristina Lacasa, Jens Lüders
Tim Stearns, Stanford University, USA27), α-Tubulin (
1:500
, SIMGA
T6199
), α-Tubulin (
1:250
,
Abcam
,
ab18251
). For the cells expressing the GFP-tagged recombinant proteins, anti-GFP antibody immunostaining was
(22)
A specific G9a inhibitor unveils BGLT3 lncRNA as a universal mediator of chemically induced fetal globin gene expression
Nature communications
January 12, 2023
Takase S, Hiroyama T, Shirai F, Maemoto Y, Nakata A, Arata M, Matsuoka S, Sonoda T, Niwa H, Sato S,
et al
.
Instruments Inc.). Primary antibodies used were
anti-G9a
(
1:1000
,
CST
, #
3306
), anti–α-tubulin (
1:5000
,
Abcam
,
ab18251
), anti-H3K9me2 (
1:3000
,
Abcam
,
ab1220
), anti-H3 (
1:5000
,
Abcam
,
ab1791
), anti-H3K27me3 (
1:3000
,
Sigma
,
(23)
α-Tubulin acetylation at lysine 40 regulates dendritic arborization and larval locomotion by promoting microtubule stability in Drosophila
PloS one
January 1, 2023
Niu X, Mao CX, Wang S, Wang X, Zhang Y, Hu J, Bi R, Liu Z, Shan J.
ImmunoResearch),
mouse
anti-α-tubulin (
1:1,000
; mAb B-5-1-2; Sigma-Aldrich), rabbit anti-α-tubulin (
1:200
;
ab18251
;
Abcam
),
mouse
anti-acetylated tubulin (
1:800
; mAb 6-11B-1; Sigma-Aldrich), anti-GFP (
1:500
;
50430-2-AP
;
(24)
Liraglutide Counteracts Endoplasmic Reticulum Stress in Palmitate-Treated Hypothalamic Neurons without Restoring Mitochondrial Homeostasis
International journal of molecular sciences
December 30, 2022
Griffin H, Sullivan SC, Barger SW, Phelan KD, Baldini G.
anti-COX IV antibody (Cat #
ab16056
), rabbit anti-GRP78/BiP (Cat #
ab21685
), rabbit anti-α-tubulin (Cat #
ab18251
) and goat anti-GFAP antibody (Cat #
ab53554
), from
Abcam
(Cambridge, MA, USA); mouse anti-KDEL antibody
(25)
ReCSAI: recursive compressed sensing artificial intelligence for confocal lifetime localization microscopy
BMC Bioinformatics
December 8, 2022
Sebastian Reinhard, Dominic A. Helmerich, Dominik Boras, Markus Sauer, Philip Kollmannsberger
\setlength{\oddsidemargin}{-69pt} \begin{document}$$\alpha$$\end{document}α-tubulin primary antibody (
Abcam
, #
ab18251
) in blocking buffer for 1 hour. After primary antibody
incubation
, cells were rinsed with PBS (Sigma-Aldrich,
(26)
Altered cytoskeletal status in the transition from proneural to mesenchymal glioblastoma subtypes
Scientific Reports
December 1, 2022
Maureen Keller, Magdalena Blom, Lei Liu Conze, Min Guo, Daniel Hägerstrand, Pontus Aspenström
antibodies (#610,153; BD Biosciences, Franklin Lakes, NJ, USA); rabbit polyclonal anti-α-tubulin antibodies (
ab18251
;
Abcam
, Cambridge, UK); rabbit polyclonal anti-GFAP antibodies (Z0334) (Agilent Technologies, Santa
(27)
Nano-positioning and tubulin conformation contribute to axonal transport regulation of mitochondria along microtubules
Proceedings of the National Academy of Sciences
November 8, 2022
Valérie Van Steenbergen, Flavie Lavoie-Cardinal, Youcef Kazwiny, Marianna Decet, Tobie Martens, Patrik Verstreken, Werend Boesmans, Paul De Koninck, Pieter Vanden Berghe
Santa Cruz Biotechnology
),
chicken
MAP2 (
1:5,000
, cat. number
ab5392
,
Abcam
), rabbit α-tubulin (
1:1,000
, cat. number
ab18251
,
Abcam
),
rat
α-tubulin (
1:50
, cat. number
MA180189
,
Invitrogen
),
chicken
beta III tubulin (
1:50
, cat.
(28)
Human SFI1 and Centrin form a complex critical for centriole architecture and ciliogenesis
The EMBO journal
November 2, 2022
Laporte MH, Bouhlel IB, Bertiaux E, Morrison CG, Giroud A, Borgers S, Azimzadeh J, Bornens M, Guichard P, Paoletti A,
et al
.
β‐tubulin (AA344, scFv‐S11B,
1:250
), and α‐tubulin (AA345, scFv‐F2C,
1:250
) (Nizak et al, 2003), α‐tubulin (
ab18251
,
Abcam
,
1:500
) and anti‐tubulin YL1/2 (
ab6160
,
Abcam
,
1:250
) were purchased from the indicated suppliers.
(29)
A role for Nup153 in nuclear assembly reveals differential requirements for targeting of nuclear envelope constituents
Molecular biology of the cell
November 1, 2022
LaJoie D, Turkmen AM, Mackay DR, Jensen CC, Aksenova V, Niwa M, Dasso M, Ullman KS.
ab124770
);
mouse
α-SUN2 (gift from Brian Burke);
rat
α-RFP (
Proteintech
;
5f8
); rabbit α-tubulin (
Abcam
;
ab18251
);
rat
α-tubulin (
Abcam
;
ab6160
);
chicken
α-tubulin (
Synaptic Systems
;
302 206
);
sheep
α-tubulin (
Cytoskeleton
;
(30)
RNase L activation in the cytoplasm induces aberrant processing of mRNAs in the nucleus
PLoS pathogens
November 1, 2022
Burke JM, Ripin N, Ferretti MB, St Clair LA, Worden-Sapper ER, Salgado F, Sawyer SL, Perera R, Lynch KW, Parker R.
antibody (
Thermo Fisher Scientific
; NB500-171) was used at
1:1000
.
Mouse
anti-alpha tubulin (
Abcam
:
ab18251
) was used at
1:1000
. Rabbit anti-GAPDH (
Cell Signaling Technology
:
2118L
) was used at
1:2000
. Anti-rabbit
(31)
Bromo-protopine, a novel protopine derivative, alleviates tau pathology by activating chaperone-mediated autophagy for Alzheimer’s disease therapy
Frontiers in Molecular Biosciences
October 26, 2022
Sravan Gopalkrishnashetty Sreenivasmurthy, Ashok Iyaswamy, Senthilkumar Krishnamoorthi, Rambabu N. Reddi, Ananth Kumar Kammala, Karthick Vasudevan, Sanjib Senapati, Zhou Zhu, Cheng-Fu Su, Jia Liu,
et al.
(Lys40),"
Thermoscientific
, Waltham, MA′ UT",#
32–2700
,WB
1:20000
α-tubulin,"
Abcam
, Waltham, MA, United States",#
ab18251
,WB
1:20000
ACTB (β-actin),"Santa Quz Biotechnology, Inc., Dallas, Texas, United States",# sc-47778,WB
(32)
Generation and characterization of human U-2 OS cell lines with the CRISPR/Cas9-edited protoporphyrinogen oxidase IX gene
Scientific reports
October 12, 2022
Novakova Z, Milosevic M, Kutil Z, Ondrakova M, Havlinova B, Kasparek P, Sandoval-Acuña C, Korandova Z, Truksa J, Vrbacky M,
et al
.
µg/ml;
sc-137093
;
Santa Cruz Biotechnology
, Dallas, TX, USA), rabbit anti-α-tubulin Ab (0.02 µg/ml;
ab18251
;
Abcam
, Cambridge, UK),
mouse
monoclonal
anti-NDUFB8
Ab (0.1 µg/ml;
ab110242
;
Abcam
, Cambridge, UK),
(33)
CA10 is associated with HBV-related hepatocarcinogenesis
Biochemistry and biophysics reports
September 1, 2022
Chung KM, Chen YT, Hong CC, Chang IC, Lin SY, Liang LY, Chen YR, Yeh CT, Huang SF.
12953-1-AP
(
1:500
);
Proteintech
, Chicago, IL, USA), α-tubulin (α-tubulin Rabbit polyclonal antibody:
ab18251
(
1:10,000
);
Abcam
, Cambridge, UK), and
GAPDH
(GAPDH Rabbit polyclonal antibody:
GTX100118
(
1:20,000
);
(34)
Evidence for a HURP/EB free mixed-nucleotide zone in kinetochore-microtubules
Nature Communications
August 10, 2022
Cédric Castrogiovanni, Alessio V. Inchingolo, Jonathan U. Harrison, Damian Dudka, Onur Sen, Nigel J. Burroughs, Andrew D. McAinsh, Patrick Meraldi
from E. Nigg23), recombinant
human
anti-α-tubulin (
1:30054
), rabbit anti-α-tubulin (
1:1000
;
Abcam
;
ab18251
),
mouse
anti-Hec1
(
1:700
;
Abcam
;
ab3613
), rabbit anti-Mad2 (
1:1000
;
Bethyl
;
A300-301A
), and mouse anti-EB1
(35)
Mechanistic basis for Sgo1-mediated centromere localization and function of the CPC
The Journal of cell biology
August 1, 2022
Abad MA, Gupta T, Hadders MA, Meppelink A, Wopken JP, Blackburn E, Zou J, Gireesh A, Buzuk L, Kelly DA,
et al
.
National Cancer Research Centre, Madrid, Spain; Serrano et al., 2009),
mouse
anti-tubulin (
1:10,000
;
ab18251
;
Abcam
), and rabbit anti-GFP (
1:1,000
; Abcam). Secondary antibodies used were
goat
anti-
mouse
680,
donkey
(36)
Targeting the HDAC6-Cilium Axis Ameliorates the Pathological Changes Associated with Retinopathy of Prematurity
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
July 1, 2022
Ran J, Zhang Y, Zhang S, Li H, Zhang L, Li Q, Qin J, Li D, Sun L, Xie S,
et al
.
were as follows: HDAC6 (
07‐732
;
Millipore
), pT845‐
ASK1
(
3765
;
Cell Signaling Technology
), α‐tubulin (
ab18251
;
Abcam
), acetylated‐α‐tubulin (
T6793
;
Sigma‐Aldrich
), and β‐actin (
A5316
,
Sigma‐Aldrich
). Statistical
(37)
The cohesin acetylation cycle controls chromatin loop length through a PDS5A brake mechanism
Nature Structural & Molecular Biology
June 16, 2022
Marjon S. van Ruiten et al.
1:1,000
), SCC4 (
Abcam
, no.
ab46906
,
1:1,000
), Actin (
Abcam
, no.
ab6276
,
1:5,000
) and Tubulin (
Abcam
, no.
ab18251
,
1:10
.000). Secondary antibodies Goat-anti-
Mouse
-PO (DAKO, no. P0447) and Goat-anti-Rabbit-PO (DAKO,
(38)
Cytotoxicity of 9,10-Phenanthrenequinone Impairs Mitotic Progression and Spindle Assembly Independent of ROS Production in HeLa Cells
Toxics
June 16, 2022
Seul Kim, Jiyeon Leem, Jeong Su Oh, Jae-Sung Kim
temperature.
HeLa cells
were incubated with anti-α-tubulin antibody (
1:1000
, T7541;
1:100
, rabbit, #
ab18251
,
Abcam
, Cambridge, MA, USA), CENP-A (
1:100
, #
ab13939
,
Abcam
), anti-p-
Aurora A
(
1:250
,
3079
,
Cell Signaling Technology
(39)
Isotropic three-dimensional dual-color super-resolution microscopy with metal-induced energy transfer
Science Advances
June 10, 2022
Jan Christoph Thiele, Marvin Jungblut, Dominic A. Helmerich, Roman Tsukanov, Anna Chizhik, Alexey I. Chizhik, Martin J. Schnermann, Markus Sauer, Oleksii Nevskyi, Jörg Enderlein
Subsequently,
microtubule
samples were incubated with rabbit anti–α-tubulin antibody (4 ng/μl;
Abcam
, no.
ab18251
), and clathrin samples were incubated with rabbit anti-clathrin antibody (4 ng/μl;
Abcam
, no.
ab21679
)
(40)
Tissue-specific inhibition of protein sumoylation uncovers diverse SUMO functions during C. elegans vulval development
PLOS Genetics
June 6, 2022
Aleksandra Fergin, Gabriel Boesch, Nadja R. Greter, Simon Berger, Alex Hajnal
used:
anti-SUMO-1
1:500
(
S5446
Sigma
), anti-Flag
1:3000
(
Sigma
F3165
-1MG),
anti-Tubulin
1:10 000
(
Abcam
ab18251
), HRPGoat anti-Rabbit
1:2000
(
Jackson
ImmunoReserach
111-035-144
) and HRP Goat anti-
Mouse
1:2000
(
Jackson
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Reviews
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(1)
3/13/2017
Antibody Against Alpha Tubulin For Immunofluorescence
Adam Kramer
This antibody is great to image the microtubule cytoskeleton in cultured mammalian cells
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Anti-alpha Tubulin antibody - Microtubule Marker from Abcam
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