HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DNMT3B (Cat# RC223206, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNMT3B antibody (Cat# TA890112). Positive lysates [LY406229] (100ug) and [LC406229] (20ug) can be purchased separately from OriGene.