HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY EPS15 (Cat# RC212097, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPS15 antibody (Cat# TA890119). Positive lysates [LY419614] (100ug) and [LC419614] (20ug) can be purchased separately from OriGene.