Rabbit (DA1E) mAb IgG XP ® Isotype Control (Alexa Fluor ® 488 Conjugate) 2975S from Cell Signaling Technology

Product Specs
ItemRabbit (DA1E) mAb IgG XP ® Isotype Control (Alexa Fluor ® 488 Conjugate)
CompanyCell Signaling Technology
Price $196.00 Inquire
Catalog Number2975S
Quantity100 µl
ApplicationsIF-IC, FC-FP, FC-L
HostRabbit
Conjugate/TagAlexa Fluor ® 488
ClonalityMonoclonal
Research AreaRelated Products
IsotypeIgG
CloneDA1E

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Functional segregation of HIF-1α and AhR controls NK cell responsiveness under hypoxiaiScienceApril 17, 2026Sebastiano Giorgetta, Francesco Cortopassi, Theodoros Chanis, Jing Ni, Margareta P. Correia, Carsten Sticht, Volker Ast, Michael Platten, Adelheid Cerwenka, Ana Stojanovic,Cell Signaling Technology,2985S IgG XP® Isotype Control Alexa Fluor™ 488 (DA1E),Cell Signaling Technology,2975 Mouse IgG2a kappa Isotype Control PerCP-eFluor™ 710 (BM2a),Thermo Fisher Scientific,46-4724-82;RRID:

(2) Heterogeneity of glucose metabolism and uptake identifies distinct cancer cell and cancer stem cell phenotypesCellular & Molecular Biology LettersJanuary 17, 2026Zuzana Tylichova, Martin Krkoska, Vaclav Hrabal, Michaela Stenckova, Borivoj Vojtesek, Philip J. CoatesA32731) for 1 h at room temperature. Appropriate isotype controls (1:100; Cell Signaling, 2985 and 2975) or secondary antibody in the absence of primary antibody were used as negative controls. Antifade Mountant

(3) EZH2 variants derived from cryptic splice sites govern distinct epigenetic patterns during embryonic developmentNucleic Acids ResearchSeptember 9, 2025Ningning Guo, Qiuxiao Guo, Hongchao Zhan, Jian Lv, Yu Fang, Jiajie Li, Junmei Wang, Die Li, Xiao Ma, Qingqing Yan, et al.4°C. After rinsing, sections were incubated with Alexa Fluor 488-conjugated secondary antibody (CST, #2975, 1:1000) for 1 h at room temperature in the dark. The sections were treated with blocking buffer again

(4) The inhibitory receptor Siglec-E controls antigen-presenting cell activation and T cell–mediated transplant rejectionScience Translational MedicineMay 7, 2025Thiago J. Borges, Karina Lima, Rodrigo B. Gassen, Kaifeng Liu, Yoshikazu Ganchiku, Guilherme T. Ribas, Minxue Liao, Joao I. B. Goncalves, Isadora T. Lape, Ivy A. Rosales, et al.64117S), or rabbit (DA1E) IgG isotype control–PE or –AF488 (Cell Signaling, catalog nos. 5742S and 2975S, respectively) for 30 min at room temperature. Stained cells were analyzed on an LSR Fortessa X-20 flow cytometer

(5) Application of AVL BOOST modeling to optimize the engine working load and drivetrain transmission ratio of the diesel firefighting pump systemHeliyonDecember 30, 2024Thong Duc Hong, Binh Nhat Nguyen, Phat Tan Truong, Son Hoang Do, Minh Quang Pham, Thang Viet VuSpecification,Value Manufacturer,Kirloskar Rated speed (rpm),2975 Rated head (m),130 Rated flow (m3/h),122 Efficiency at duty point (%),65.86 Liquid handled,Water Rated

(6) MAM‐STAT3‐Driven Mitochondrial Ca+2 Upregulation Contributes to Immunosenescence in Type A Mandibuloacral Dysplasia PatientsAdvanced ScienceDecember 11, 2024Arshad Ahmed Padhiar, Xiaohong Yang, Syed Aqib Ali Zaidi, Zhu Li, Jinqi Liao, Wei Shu, Arif Ali Chishti, Liangge He, Gulzar Alam, Abdullah Faqeer, et al.purchased from Cell Signaling Technology and diluted at 1:800: rabbit Alexa Fluor‐488 antibodies (cat. no. 2975S), rabbit Alexa Fluor‐594 antibodies (cat. no. 8760S), mouse Alexa Fluor‐594 antibodies (cat. no. 8527S)

(7) A MYCN-driven de-differentiation profile identifies a subgroup of aggressive retinoblastomaCommunications BiologyJuly 30, 2024Tatsiana Ryl, Elena Afanasyeva, Till Hartmann, Melanie Schwermer, Markus Schneider, Christopher Schröder, Maren Wagemanns, Arthur Bister, Deniz Kanber, Laura Steenpass, et al.Cross-Adsorbed Secondary Antibody, Thermo Fisher Scientific, and Rabbit mAb IgG XP®Isotype Control (#2975 Cell Signaling). Cells (106) were prefixed with 4% paraformaldehyde (Morphisto) for 15 min at room temperature,

(8) CCAAT enhancer binding protein delta activates vesicle associated membrane protein 3 transcription to enhance chemoresistance and extracellular PD-L1 expression in triple-negative breast cancerJournal of experimental & clinical cancer research : CRApril 16, 2024Zhao Y, Yu Y, Li X, Guo A.Cell Signaling Technology) at 4℃ overnight, followed by incubation with Alexa Fluor 488-conjugated secondary (#2975, Cell Signaling Technology) at room temperature for 40 min. The staining was analyzed under fluorescence microscopy

(9) Identification of miR-20b-5p as an inhibitory regulator in cardiac differentiation via TET2 and DNA hydroxymethylationClinical epigeneticsMarch 15, 2024Li KX, Li JR, Zuo SJ, Li X, Chen XT, Xiao PY, Li HT, Sun L, Qian T, Zhang HM, et al.Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate) (diluted 1:1000, Cell Signaling Technology, #2975). The samples were acquired using Amnis Image StreamX MarkII flow cytometer (Merck & Millipore, Germany)

(10) Disease-associated astrocyte epigenetic memory promotes CNS pathologyNatureMarch 1, 2024Lee HG, Rone JM, Li Z, Akl CF, Shin SW, Lee JH, Flausino LE, Pernin F, Chao CC, Kleemann KL, et al.rabbit anti-H3K27ac (Abcam, 1:100, ab4729), and rabbit IgG polyclonal isotype control (Cell Signaling, 2975 S, 1:100). PCR primers were designed with Primer375 to generate 50–150 bp amplicons. Primer sequences

(11) Signaling via a CD27-TRAF2-SHP-1 axis during naive T cell activation promotes memory-associated gene regulatory networksImmunityFebruary 13, 2024Carla A. Jaeger-Ruckstuhl, Yun Lo, Elena Fulton, Olivia G. Waltner, Tamer B. Shabaneh, Sylvain Simon, Pranav V. Muthuraman, Colin E. Correnti, Oliver J. Newsom, Ian A. Engstrom, et al.AB_2943643 "Alexa Fluor 488 Rabbit mAb IgG XP Isotype Control (clone DA1E, 1:1000)",Cell Signaling Technology,Cat#2975; RRID: AB_10699151 "Pacific Blue mouse anti-human CD8 (clone RPA-T8, 1:100)",BD Biosciences,Cat#558207;

(12) Targeting FAcilitates Chromatin Transcription complex inhibits pleural mesothelioma and enhances immunotherapyJournal of Experimental & Clinical Cancer ResearchNovember 16, 2023Anand Singh, Nathanael Pruett, Shivani Dixit, Sudheer K. Gara, Haitao Wang, Roma Pahwa, David S. Schrump, Chuong D. HoangRabbit (DA1E) mAb IgG XP®, Alexa Fluor® 488 conjugated, isotype control (Cell signaling technology, #2975) according to manufacturer’s protocols, and analyzed by flow cytometry. PD-L1 Flow cytometry analysis PD-L1

(13) Imetelstat-mediated alterations in fatty acid metabolism to induce ferroptosis as a therapeutic strategy for acute myeloid leukemiaNature CancerOctober 30, 2023Claudia Bruedigam, Amy H. Porter, Axia Song, Gerjanne Vroeg in de Wei, Thomas Stoll, Jasmin Straube, Leanne Cooper, Guidan Cheng, Vivian F. S. Kahl, Alexander P. Sobinoff, et al.(rabbit monoclonal antibody IgG XP Isotype Control Alexa Fluor 488 conjugate, Cell Signaling; cat. 2975; clone DA1E) at a dilution of 1:400 in 1% BSA–PBS for 1 h at room temperature or O/N at 4 °C. Cytospins

(14) PM2.5 induced liver lipid metabolic disorders in C57BL/6J miceFront Endocrinol (Lausanne)September 14, 2023Chenxiao Zhang, Tengfei Ma, Chang Liu, Ding Ma, Jian Wang, Meng Liu, Jinjun Ran, Xueting Wang, Xiaobei Dengincubated overnight. Slides were incubated with Anti-rabbit or anti-mouse IgG secondary antibody (#2975, #4408, 1:500, Cell Signaling Technology, USA) for 1 h, followed by nuclear staining with 4’,6-diamidino-2-phenylindole

(15) Irreversible HER2 inhibitors overcome resistance to the RSL3 ferroptosis inducer in non-HER2 amplified luminal breast cancerCell Death DisAugust 18, 2023Soon Young Park, Kang Jin Jeong, Alfonso Poire, Dong Zhang, Yiu Huen Tsang, Aurora S. Blucher, Gordon B. Millscorresponding isotype control antibody (Thermo Fisher Scientific, 11-4714-42; Cell Signaling Technology, 2975S) for 20 min at RT. For SLC7A11, this was followed by secondary antibody incubation for 20 min. Cells

(16) Adding liposomal doxorubicin enhances the abscopal effect induced by radiation/αPD1 therapy depending on tumor cell mitochondrial DNA and cGAS/STINGJournal for immunotherapy of cancerAugust 1, 2023Wang L, Luo R, Onyshchenko K, Rao X, Wang M, Menz B, Gaedicke S, Grosu AL, Firat E, Niedermann G.(clone Moushi101) from Invitrogen; Phospho-Histone H2A.X-AF488 (#9719) and Isotype Control-AF488 (#2975) were from Cell signaling. 7-AAD (BD Bioscience), Zombie Red, and Zombie NIR (BioLegend) were used to

(17) Stage-specific dual function: EZH2 regulates human erythropoiesis by eliciting histone and non-histone methylationHaematologicaApril 6, 2023Mengjia Li, Donghao Liu, Fumin Xue, Hengchao Zhang, Qianqian Yang, Lei Sun, Xiaoli Qu, Xiuyun Wu, Huizhi Zhao, Jing Liu, et al.9733S) for 20 min, then incubated with anti-rabbit IgG Alexa fluor 488 (Cell Signaling Technology, 2975) for 20 min, followed by fluorescence-activated cell sorting (FACS) analysis using a BD LSRFortessa™

(18) BMAL1-TTK-H2Bub1 loop deficiency contributes to impaired BM-MSC-mediated bone formation in senile osteoporosisMolecular therapy. Nucleic acidsMarch 14, 2023Jinteng L, Peitao X, Wenhui Y, Guiwen Y, Feng Y, Xiaojun X, Zepeng S, Jiajie L, Yunshu C, Zhaoqiang Z, et al.the samples overnight at 4°C. The secondary antibodies used were anti-rabbit Alexa 488 (catalog no. 2975; Cell Signaling Technology), anti-mouse Alexa 488 (catalog no. 4408; Cell Signaling Technology), anti-rabbit

(19) BH3 mimetics in combination with nilotinib or ponatinib represent a promising therapeutic strategy in blast phase chronic myeloid leukemiaCell Death DiscovNovember 15, 2022Narissa Parry, Caroline Busch, Victoria AÃŸmann, Jennifer Cassels, Alan Hair, G. Vignir Helgason, Helen Wheadon, Mhairi Copland2985), and Rabbit (DA1E) mAb IgG XP® Isotype Control – AlexaFluor® 488 (Cell Signaling Technology, cat. 2975). Data were captured using a BD FACSCanto™ II (BD Biosciences) and analyses were carried out using FlowJo

(20) Age-related Loss of miR-124 Causes Cognitive Deficits via Derepressing RyR3 ExpressionAging and diseaseOctober 1, 2022Liu K, Yin Y, Le Y, Ouyang W, Pan A, Huang J, Xie Z, Zhu Q, Tong J.were used to visualize the sections. Primary antibodies were controlled by isotype IgG (CST, cat#: 2975S, USA) to validate antibody specificity. Based on the Iba1 staining, the resting and activated microglia

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Rabbit (DA1E) mAb IgG XP ® Isotype Control (Alexa Fluor ® 488 Conjugate)

Rabbit (DA1E) mAb IgG XP ® Isotype Control (Alexa Fluor ® 488 Conjugate) from Cell Signaling Technology

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Cell Signaling Technology

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Rabbit (DA1E) mAb IgG XP ® Isotype Control (Alexa Fluor ® 488 Conjugate) from Cell Signaling Technology

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