HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CYB5R3 (Cat# RC229902, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYB5R3 antibody (Cat# TA890109). Positive lysates [LY400140] (100ug) and [LC400140] (20ug) can be purchased separately from OriGene.