HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MAP3K5 (Cat# RC209913, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP3K5 antibody (Cat# TA890021). Positive lysates [LY401794] (100ug) and [LC401794] (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from different cell lines or tissues by using anti-MAP3K5 rabbit polyclonal antibody (TA890021).
Supplier Page from OriGene Technologies for ASK1 (MAP3K5) Rabbit Polyclonal Antibody