WB: The mouse brain tissue lysate resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with Rabbit anti-PTEN (CT) antibody (Lane 1) at 1:500, or with a pre-incubation of immunizing peptide (lane 2)
IHC: Human prostate tissue stained with Anti-PTEN antibody, at 1:100 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Supplier Page from OriGene Technologies for PTEN Rabbit Polyclonal Antibody