IL-12 Antibody AF-419-NA from R&D Systems, a Bio-Techne Brand

Product Specs
ItemIL-12 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $569.00Supplier Page View Company Product Page
Catalog NumberAF-419-NA
ApplicationsBioassay,Immunoassay Development,Western Blot,Neutralization,Functional Assay
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityMouse
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
HostGoat
TargetIL-12
IsotypeIgG
ClonalityPolyclonal
ImmunogenS. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL-12, Mouse IL-12 heterodimer: Mouse IL-12 p40 (Met23-Ser335) Accession # P43432, Mouse IL-12 p35 (Arg23-Ala215) Accession # NP_032377
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Nameinterleukin 12A
NCBI Gene AliasesCLMF, IL-12A, NFSK, NKSF1, P35

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse IL-12 in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing and reducing conditions), this antibody shows less than 10% cross-reactivity with recombinant human IL-12.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Calycosin suppresses the activating effect of granulocyte-macrophage-colony-stimulating factor-producing T helper cells on macrophages in experimental atherosclerosisFrontiers in pharmacologyJanuary 1, 2025Xiong X, Huang W, Yang X, Wang X, Wu B, Li D.10 μg/mL IFN-γ neutralizing antibody (MAB485-SP, R&D Systems), 10 μg/mL IL-12 neutralizing antibody (AF-419-NA, R&D Systems), 5 μg/mL IL-4 neutralizing antibody (AF-404-SP, R&D Systems), and 2 ng/mL recombinant

(2) Distinct RORγt-dependent Th17 immune responses are required for autoimmune pathogenesis and protection against bacterial infectionCell reportsNovember 26, 2024Zhong X, Wu H, Zhang W, Shi Y, Gwack Y, Xue HH, Sun Z.IL-1β,R&D Systems,Cat# AF-401-NA; RRID:AB_416684 Goat polyclonal anti-mouse IL-12,R&D Systems,Cat# AF-419-NA; RRID:AB_354485 Goat polyclonal anti-mouse TNF-α,R&D Systems,Cat# AF-410-NA; RRID:AB_354479 Bacterial

(3) Acquired immunostimulatory phenotype of migratory CD103+ DCs promotes alloimmunity following corneal transplantationJCI insightOctober 22, 2024Blanco T, Nakagawa H, Musayeva A, Krauthammer M, Singh RB, Narimatsu A, Ge H, Shoushtari SI, Dana R.

(4) Oleic acid availability impacts thymocyte preprogramming and subsequent peripheral Treg cell differentiationNature ImmunologyJanuary 1, 2024Liangyu Lin, Mingyuan Hu, Qing Li, Liming Du, Li Lin, Yueqing Xue, Fanjun Zheng, Fei Wang, Keli Liu, Yu Wang, et al.404-ML-050), anti-IFNγ (10 μg/mL, R&D systems, cat no. MAB4851), and anti-IL-12(10 μg/mL, R&D systems, cat no. AF-419-NA) were supplied to culture medium. For Th17 differentiation, anti-CD3 (5 μg/mL, Thermo Fisher Scientific,

(5) Targeting Xcr1 on Dendritic Cells Rapidly Induce Th1-Associated Immune Responses That Contribute to Protection Against Influenza InfectionFrontiers in immunologyJanuary 1, 2022Tesfaye DY, Bobic S, Lysén A, Huszthy PC, Gudjonsson A, Braathen R, Bogen B, Fossum E.

(6) Activating a collaborative innate-adaptive immune response to control metastasisCancer cellOctober 11, 2021Sun L, Kees T, Almeida AS, Liu B, He XY, Ng D, Han X, Spector DL, McNeish IA, Gimotty P, et al.anti-rabbit IgG secondary antibody,Invitrogen,Cat #A11070 Goat polyclonal anti-Mouse IL-12,R&D Systems,Cat #AF-419-NA Rat monoclonal anti-Mouse TNF-alpha,R&D Systems,Cat #MAB4101 Goat polyclonal anti-Mouse CXCL9/MIG,R&D Systems

(7) Exosomes Derived From Septic Mouse Serum Modulate Immune Responses via Exosome-Associated CytokinesFrontiers in immunologyJanuary 1, 2019Gao K, Jin J, Huang C, Li J, Luo H, Li L, Huang Y, Jiang Y.

(8) TLR Stimulation during T-cell Activation Lowers PD-1 Expression on CD8(+) T CellsCancer immunology researchNovember 1, 2018Zahm CD, Colluru VT, McIlwain SJ, Ong IM, McNeel DG.

(9) The inhibition of malignant melanoma cell invasion of bone by the TLR7 agonist R848 is dependent upon pro-inflammatory cytokines produced by bone marrow macrophagesOncotargetJuly 6, 2018Manome Y, Suzuki D, Mochizuki A, Saito E, Sasa K, Yoshimura K, Inoue T, Takami M, Inagaki K, Funatsu T, et al.IFN-γ, #485-MI), and neutralizing antibodies (normal goat IgG control, #AB-108-C; mouse IL-6 antibody, #AF-419-NA; mouse IL-12 antibody, #AF-419-NA; mouse IFN-γ antibody, #AF-485-NA) were purchased from R&D Systems

(10) Crucial role of OX40/OX40L signaling in a murine model of asthmaMolecular medicine reportsMarch 1, 2018Lei W, Zeng D, Liu G, Zhu Y, Wang J, Wu H, Jiang J, Huang J.

(11) Efficient expression of bioactive murine IL12 as a self-processing P2A polypeptide driven by inflammation-regulated promoters in tumor cell linesCancer gene therapyNovember 1, 2015Lorenzo C, Pérez-Chacón G, Garaulet G, Mallorquín Z, Zapata JM, Rodríguez A.ﬁrst antibody solution: anti-mouse IL12 antibody (0.1%, v/v; R&D Systems, Minneapolis, MN, USA; cat. #AF-419-NA), rabbit polyclonal anti-2A peptide (0.05%, v/v; Millipore, Billerica, MA, USA; cat. #ABS31); mouse

(12) The p40 subunit of interleukin (IL)-12 promotes stabilization and export of the p35 subunit: implications for improved IL-12 cytokine productionThe Journal of biological chemistryMarch 1, 2013Jalah R, Rosati M, Ganneru B, Pilkington GR, Valentin A, Kulkarni V, Bergamaschi C, Chowdhury B, Zhang GM, Beach RK, et al.AF219-NA; cross-reactive with rhesus IL-12p70), and the polyclonal murine IL-12p70 antibody (catalog no. AF419-NA, R&D Systems (Minneapolis, MN)) were used. The FLAG-tagged proteins were detected by the monoclonal

(13) Viral antigen mediated NKp46 activation of NK cells results in tumor rejection via NK-DC crosstalkOncoimmunologySeptember 1, 2012Chinnery F, King CA, Elliott T, Bateman AR, James E.B16.OVA transfected with HN or control plasmid DNA for 24 h. Blocking anti-IL-12 antibody (R&D Systems, AF-419-NA) was also added to cultures (10 μg/ml) to assess the role of IL-12 in the response. IFNγ and IL-12 was

(14) The C5a receptor impairs IL-12-dependent clearance of Porphyromonas gingivalis and is required for induction of periodontal bone lossJournal of immunology (Baltimore, Md. : 1950)January 15, 2011Liang S, Krauss JL, Domon H, McIntosh ML, Hosur KB, Qu H, Li F, Tzekou A, Lambris JD, Hajishengallis G.

(15) Expression of bioactive single-chain murine IL-12 in transgenic plantsJournal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine ResearchJune 1, 2008Liu J, Dolan MC, Reidy M, Cramer CL.

(16) Toll-like receptor (TLR)2 and TLR3 synergy and cross-inhibition in murine myeloid dendritic cellsImmunology lettersFebruary 15, 2008Vanhoutte F, Paget C, Breuilh L, Fontaine J, Vendeville C, Goriely S, Ryffel B, Faveeuw C, Trottein F.

(17) Complement receptor 3 blockade promotes IL-12-mediated clearance of Porphyromonas gingivalis and negates its virulence in vivoJournal of immunology (Baltimore, Md. : 1950)August 15, 2007Hajishengallis G, Shakhatreh MA, Wang M, Liang S.

(18) TLR4 mediates vaccine-induced protective cellular immunity to Bordetella pertussis: role of IL-17-producing T cellsJournal of immunology (Baltimore, Md. : 1950)December 1, 2006Higgins SC, Jarnicki AG, Lavelle EC, Mills KH.cultures. The anti-IL-12 Ab was a polyclonal Ab, raised against IL-12, and neutralizes IL-12 (R&D Systems; Ab AF-419-NA specification sheet). We found that it neutralizes IL-12, but not IL-23, presumably by virtue of its

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IL-12 Antibody

IL-12 Antibody

IL-12 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (18)

Reviews

IL-12 Antibody from R&D Systems, a Bio-Techne Brand

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