Anti-CD3 antibody [SP7] ab16669 from Abcam

Product Specs
ItemAnti-CD3 antibody [SP7]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab16669
Antibody TypePrimary
Quantity1 ML, 100 UL, 500 UL
HostRabbit
ClonalityMonoclonal
CloneSP7
ApplicationsFlow Cytometry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Multiplex immunohistochemistry, Western blot, Flow Cytometry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Multiplex immunohistochemistry, Western blot, Flow Cytometry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Multiplex immunohistochemistry, Western blot
IsotypeIgG
Conjugate/TagUnconjugated
Research AreaImmuno-oncology
Gene NameCD3E
ReactivityHuman, Mouse, Rat
TargetCD3

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Sex Differences in Left-Ventricular Strain in a Murine Model of Coxsackievirus B3 MyocarditisiScienceDecember 15, 2023Damian N. Di Florio, Logan P. Macomb, Presley G. Giresi, Danielle J. Beetler, Natalie E. Bonvie-Hill, Katie A. Shapiro, Abdel-Rahman N. Naser, Sami Khatib, Emily R. Whelan, Gabriel J. Weigel, et al.103102, 1:200, rat), CD11b (Abcam, Cambridge, United Kingdom, ab133357, 1:3000, rabbit), CD3 (Abcam, ab16669, 1:200, rabbit), and F4/80 (BioRad, Hercules, CA, MCA497G, 1:250, rat). A Envision+ anti-rabbit labeled

(2) Components of the tumor immune microenvironment based on m-IHC correlate with prognosis and subtype of triple-negative breast cancerCancer MedDecember 7, 2023Luyi Lin, Haiming Li, Xin Wang, Zezhou Wang, Guanhua Su, Jiayin Zhou, Shiyun Sun, Xiaowen Ma, Yan Chen, Chao You, et al.[C‐11] antibodies to pancytokeratin (ab7753, Abcam, 1:150), rabbit monoclonal [SP7] antibodies to CD3 (ab16669, Abcam, 1:150), rabbit monoclonal [EPR22102‐37] antibodies to FoxP3 (ab215206, Abcam, 1:150), and DAPI.

(3) Heterogeneity in tertiary lymphoid structures predicts distinct prognosis and immune microenvironment characterizations of clear cell renal cell carcinomaJ Immunother CancerDecember 1, 2023Wenhao Xu, Jiahe Lu, Wang-Rui Liu, Aihetaimujiang Anwaier, Yuhao Wu, Xi Tian, Jia-Qi Su, Yuan-Yuan Qu, Jianfeng Yang, Hailiang Zhang, et al.histological features resembling lymphoid tissues with B cells (CD20, ab64088, Abcam, USA), T cells (CD3, ab16669, Abcam), follicular DCs (FDCs) (CD21, ab7290, Abcam), and germinal center (GC) cells (CD23, ab16702,

(4) Cooperation of MLL1 and Jun in controlling H3K4me3 on enhancers in colorectal cancerGenome BiologyNovember 27, 2023Xiang Lin, Ji-Dong Chen, Chen-Yu Wang, Zhen Cai, Rui Zhan, Chen Yang, La-Ying Zhang, Lian-Yun Li, Yong Xiao, Ming-Kai Chen, et al.RRID: AB_2118291), MLL1 (CST, 14,197, RRID:AB_2688010), JUN (CST, 9165, RRID:AB_2130165), CD3 (Abcam, ab16669, RRID:AB_443425), CD4 (Abcam, ab183685, RRID:AB_2686917), CD8 (Abcam, ab217344, RRID:AB_2890649), F4/80(CST,

(5) Short salsalate administration affects cell proliferation, metabolism, and inflammation in polycystic kidney diseaseiScienceNovember 17, 2023Kanhai AA, Sánchez-López E, Kuipers TB, van Klinken JB, Dijkstra KL, van der Veen I, Baelde HJ, Song X, Pei Y, Mei H, et al.RRID: AB_442102 Rat anti-F4/80 primary antibody,Serotec,N/A Rabbit anti-CD3 primary antibody,Abcam,ab16669; RRID: AB_443425 Rat anti-CD19 primary antibody,ThermoFisher Scientific,14-0194-82; RRID: AB_2637171 anti-rat

(6) YAP1/Piezo1 involve in the dynamic changes of lymphatic vessels in UVR-induced photoaging progress to squamous cell carcinomaJournal of Translational MedicineNovember 16, 2023Yuling L. Yang, Chu Zhou, Qi Chen, Shuzhan Z. Shen, Jiandan D. Li, Xiuli L. Wang, Peiru R. Wangtissues labeling with LYVE1 (1:200; Abcam ab219556), CD31 (1:100; abcam, ab222783), CD3 (1:200; Abcam ab16669), CD4 (1:100; abcam, ab183635), VE-CAD (1:100, affinity, AF6265), Piezo1 (1:100; affinity, DF12083),

(7) Histological Characteristics of Conjunctiva-Associated Lymphoid Tissue in Young and Adult Holstein CattleAnimals (Basel)November 11, 2023Keigo Kosenda, Osamu Ichii, Yusuke Yamashita, Hiromichi Ohtsuka, Shigeo Fukuda, Yasuhiro KonAntibody,Dilution,Blocking,Antigen Retrieval,Secondary Antibody CD3,"Rabbit monoclonal antibodies, ab16669 (Abcam)",1:800,10% NGS,"TB 115 °C, 15 min","Goat anti-rabbit (SABPO kit, Nichirei)" CD20,"Rabbit polyclonal

(8) MAP3K19 Affects TWEAK-Induced Response in Cultured Bronchial Epithelial Cells and Regulates Allergic Airway Inflammation in an Asthma Murine ModelCurrent Issues in Molecular BiologyNovember 8, 2023Yuuki Sandhu, Norihiro Harada, Sonoko Harada, Takayasu Nishimaki, Hitoshi Sasano, Yuki Tanabe, Tomohito Takeshige, Kei Matsuno, Ayako Ishimori, Yoko Katsura, et al.(Thr183/Tyr185) mAbs were obtained from Cell Signaling Technology (Beverly, MA, USA). Anti-CD3 mAbs (ab16669) and anti-MAP3K19 polyclonal antibodies (A14340) were obtained from Abcam (Cambridge, MA, USA). Bronchial

(9) Immunomodulatory and pro-oncologic effects of ketamine and isoflurane anesthetics in a murine modelPLOS ONEOctober 10, 2023Dominique Abrahams, Arig Ibrahim-Hashim, Robert S. Ackerman, Joel S. Brown, Christopher J. Whelan, Megan B. Garfinkel, Robert A. Gatenby, Aaron R. Munceymethod was used in Cell Conditioning 1 Mild (Ventana). The primary rabbit antibody that reacts to CD3 (ab16669, Abcam, Cambridge, MA) was used at a 1:200 dilution in Dako antibody diluent (Carpenteria, CA) and incubated

(10) Hepatorenal pathologies in TNF-transgenic mouse model of rheumatoid arthritis are alleviated by anti-TNF treatmentArthritis Research & TherapyOctober 2, 2023Xuefei Li, Yi Wang, Ziqiang Chen, Ming Ruan, Can Yang, Maolin Zhou, Ning Li, Lianping Xing, Hao Xu, Ling Yang, et al.AB5320), mouse nephrin antibody (R&D Systems, AF3159), anti-CD45R (Abcam, ab64100), anti-CD3 (Abcam, ab16669), anti-iNOS (Abcam, ab3523), and anti-F4/80 (Abcam, ab6640). After washing with phosphate buffer, the

(11) Microenvironment-dependent growth of Sezary cells in humanized IL-15 miceDisease Models & MechanismsOctober 1, 2023Jie Gao, Shumei Ren, Gabrielle Choonoo, Guoying Chen, Davor Frleta, Jun Zhong, Namita Gupta, Prachi Sharma, Adelekan Oyejide, Gurinder S. Atwal, et al.paraffin. Sections (4 μm) were cut and stained with Hematoxylin and Eosin (H&E), anti-human CD3 (Abcam, ab16669, clone SP7; 1:100) and 5 μg/ml anti-human PD-1 (Abcam, ab52587, clone NAT105; 1:200) at 4°C overnight. 5′

(12) Intratumoral microbial heterogeneity affected tumor immune microenvironment and determined clinical outcome of HBV-related HCCHepatologyOctober 1, 2023Shengnan Li, Han Xia, Zeyu Wang, Xiehua Zhang, Tianqiang Song, Jia Li, Liang Xu, Ningning Zhang, Shu Fan, Qian Li, et al.and immunohistochemistry staining. Sections were incubated with monoclonal antibodies against CD3 (ab16669, Abcam), CD8 (ab93278, Abcam), CD68 (ZM-0060, ZSBio), CD163 (ZM-0428, ZSBio), and PD-L1 (ab210931, Abcam)

(13) Intranasal inoculation of female BALB/c mice with replication-deficient human adenovirus type 5 expressing SARS-CoV‐2 nucleocapsid protein aggravates lung pathology upon re-encountering the antigenVirus ResearchOctober 1, 2023Junxia Cao, Hongjing Gu, Xueting Zhang, Hongfang Yun, Jiarong Li, Chuan-Yimu Si, Jiyan Zhang, Hui Wangapplied in this study include: P-MLKL (1:100, Cat# NBP2-66953; Novus Biologicals), CD3 (1:200, Cat# ab16669; Abcam), CD68 (1:300, Cat# 97778; Cell Signaling), CD103 (1:400, Cat# ab224202; Abcam), Ly6G (1:100,

(14) Fasting mimicking diet in mice delays cancer growth and reduces immunotherapy-associated cardiovascular and systemic side effectsNature CommunicationsSeptember 8, 2023S. Cortellino, V. Quagliariello, G. Delfanti, O. Blaževitš, C. Chiodoni, N. Maurea, A. Di Mauro, F. Tatangelo, F. Pisati, A. Shmahala, et al.(CD152),BioXcell,BP0032 anti-mouse PD-1,BioXcell,BE0146 Antibodies IHC,Antibodies IHC,Antibodies IHC Anti-CD3,Abcam,Ab16669 Anti- CD8α,Abcam,Ab217344 Anti-Myeloperoxidase,Abcam,Ab208670 Anti-B220,Bd bioscience,553928

(15) Chronodisruption of the acute inflammatory response by night lighting in ratsScientific ReportsAugust 29, 2023Viera Jerigova, Michal Zeman, Monika Okuliarova(Vector Laboratories, Burlingame, CA, USA). The sections were stained with rabbit anti-CD3 primary antibody (ab16669, Abcam; diluted 1:300) overnight at 4 °C and biotinylated anti-rabbit IgG secondary antibody (BA-1000,

(16) Driver gene combinations dictate cutaneous squamous cell carcinoma disease continuum progressionNat CommunAugust 25, 2023Peter Bailey, Rachel A. Ridgway, Patrizia Cammareri, Mairi Treanor-Taylor, Ulla-Maja Bailey, Christina Schoenherr, Max Bone, Daniel Schreyer, Karin Purdie, Jason Thomson, et al.heated at 60 °C for 2 h. The following antibodies were used on a Leica Bond Rx autostainer: CD3 (Abcam, ab16669, clone SP7, 1:100), CD4 (eBioscience, 14-9766-82, clone 4SM95 1:500), CD8 (eBioscience, 14-0808-82,

(17) CLDN18.2 expression and its impact on prognosis and the immune microenvironment in gastric cancerBMC GastroenterolAugust 16, 2023Canming Wang, Yukai Wang, Jinxia Chen, Yi Wang, Chuhong Pang, Chen Liang, Li Yuan, Yubo MaThe antibodies used were against CLDN18.2 (Abcam, ab222512, dilution ratio 1:200), CD3 + T (Abcam, ab16669, dilution ratio 1:200), CD4 + T (Abcam, ab133616, dilution ratio 1:200), CD8 + T (Abcam, ab17147, dilution

(18) Treating solid tumors with TCR-based chimeric antigen receptor targeting extra domain B-containing fibronectinJournal for ImmunoTherapy of CancerAugust 16, 2023Zhijie Zhang, Chang Liu, Muhan Wang, Rongcheng Sun, Zhe Yang, Zhen Hua, Yushuang Wu, Mengting Wu, Hang Wang, Wen Qiu, et al.infusion. IHC for infiltrating T cells was performed as previously described34 using an anti-CD3 antibody (ab16669, Abcam). Blood vessels in the tumor tissues were detected using an anti-CD31 antibody (A19014, ABclonal). Test

(19) Antigen-dependent IL-12 signaling in CAR T cells promotes regional to systemic disease targetingNature communicationsAugust 7, 2023Lee EHJ, Murad JP, Christian L, Gibson J, Yamaguchi Y, Cullen C, Gumber D, Park AK, Young C, Monroy I, et al.mouse anti-human CD8 (DAKO), and mouse anti-human TAG72 (AB16838, Abcam), and rabbit anti-mouse CD3 (AB16669, Abcam). Images were obtained using the Nanozoomer 2.0HT digital slide scanner and the associated NDP.view2

(20) DNA hypermethylation driven by DNMT1 and DNMT3A favors tumor immune escape contributing to the aggressiveness of adrenocortical carcinomaClin EpigeneticsAugust 2, 2023Gwenneg Kerdivel, Floriane Amrouche, Marie-Ange Calmejane, Floriane Carallis, Juliette Hamroune, Constanze Hantel, Jérôme Bertherat, Guillaume Assié, Valentina Boevaimmunostained using the Bond Polymere Refine kit (Leica, Wetzlar, Germany). The antibody used was anti-CD3 ab16669 (rabbit polyclonal, Abcam). Slides were scanned with a Lamina slide scanner (PerkinElmer, Waltham, MA,

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(1)

5/20/2022Great Anti-CD3 Antibody for Immunofluorescent Staining for Paraffin Tissue Lucy LiuWe used this antibody to study the T cell infiltration in a TUBO breast cancer mouse model. This anti-CD3 antibody has worked well for immunofluorescent staining for paraffin tissue.
(1)

7/6/2016A Great Alternative to Mouse Antibodies to Make Your Immunofluorescence Life Easier Jennifer MarshallI needed to be able to fit CD3 into my existing antibody panel for immunoflourescence within my tissue. As I study human tissue the antibodies that I was already using were IgG1, 2a, 2b and rat. As I ... Read Review

Anti-CD3 antibody [SP7] from Abcam

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Anti-CD3 antibody [SP7] from Abcam

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