HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SCNN1A (Cat# RC228555, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCNN1A antibody(Cat# TA890149). Positive lysates [LY422000] (100ug) and [LC422000] (20ug) can be purchased separately from OriGene.