HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDX58 (RC217615, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX58 r. Positive lysates LY415370 (100ug) and LC415370 (20ug) can be purchased separately from OriGene.