Mouse CLEC4F/CLECSF13 Antibody AF2784 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMouse CLEC4F/CLECSF13 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF2784
NCBI Gene AliasesKclr, KUCR_MOUSE, Clecsf13
ApplicationsFlow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunocytochemistry/ Immunofluorescence
ReactivityMouse,Transgenic Mouse
TargetCLEC4F/CLECSF13
Conjugate/TagUnconjugated
HostGoat
IsotypeIgG
Quantity100 ug (also 25 ug)
NCBI Full Gene NameC-type lectin domain family 4, member f
ClonalityPolyclonal
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse CLEC4F/CLECSF13, Ala65-Gly548, Accession # P70194
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

Can this antibody be used to study Kupffer cells in mouse liver tissue?

Yes, the CLEC4F/CLECSF13 antibody has been shown to specifically detect Kupffer cells in mouse liver tissue, as evidenced by its use in immunohistochemistry and flow cytometry in various studies, including those examining liver injury and inflammation (, ).

Is there any evidence of this antibody being used in studies related to liver diseases?

The CLEC4F/CLECSF13 antibody has been utilized in research focusing on liver diseases, such as non-alcoholic steatohepatitis (NASH) and atherosclerosis, where it helped identify macrophage populations and their roles in liver pathology (, ).

What applications has this antibody been validated for in scientific research?

The CLEC4F/CLECSF13 antibody has been shown to work in several applications, including Western blotting, flow cytometry, immunocytochemistry, immunofluorescence, and immunohistochemistry, making it versatile for various experimental setups (, ).

How does this antibody contribute to understanding macrophage behavior in liver conditions?

The CLEC4F/CLECSF13 antibody allows researchers to analyze the differentiation and inflammatory responses of Kupffer cells and other macrophage populations in the liver, providing insights into their roles during conditions such as hypercholesterolemia and liver injury (, ).

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Chronic stress disrupts hepatic homeostasis and accelerates liver cancer progression through ADRB2 signalingSci AdvMay 1, 2026Huimin Qin, Liuwei Chu, Xiaying Zheng, Huimei Zhang, Yue Lan, Ji Hu, Lu Li(1:500; Abcam, ab182136), Ki67 (1:1000; Cell Signaling Technology, 9129), CLEC4F (1:200; R&D Systems, AF2784), F4/80 (1:1000; Cell Signaling Technology, 70076), and VSIG4 (1:500; Abcam, ab252933). Primary antibody

(2) MafB is a conserved transcriptional regulator of macrophage development and functional identity across tissues and speciesImmunityMarch 10, 2026Domien Vanneste, Wen Peng, Zhuangzhuang Liu, Malik Hamaïdia, Raphaël La Rocca, Joan Abinet, Alexis Balthazar, Fabienne Perin, Alexandre Hego, Didier Cataldo, et al.conjugated",BioLegend,Cat#146104; RRID: AB_2562383 "Anti-mouse Clec4f Polyclonal Antibody(Goat), unconjugated",R&D Systems,Cat# AF2784; RRID: AB_2081339 "Anti-mouse F4/80 Monoclonal Antibody(Rat, BM8), FITC conjugated",BioLegend,Cat#123108;

(3) Palmitoylation of Tfr1 enhances platelet ferroptosis and liver injury in heat strokeActa Pharmaceutica Sinica BJanuary 1, 2026Qiyuan An, Riqing Wei, Zhicheng Huang, Youyong Tang, Minghao Wang, Sixiao He, Kaihua Huang, Zhifeng Liu, Meimei Zhang, Ru Li, et al.eliminate endogenous peroxidase, and 5% BSA to block non-specific antigens. After blocking, Clec4f (AF2784, R&D Systems, Minneapolis, MN, USA), Glutamine Synthetase (ab73593, Abcam), CD42b (ab183345, Abcam)

(4) Bile acid receptor Tgr5 prevents macrophage hyperinflammation during bacterial sepsis through metabolic and epigenetic silencingiScienceDecember 19, 2025Maria Reich, Tobias Franz, Haifeng C. Xu, Paulina Philippski, Jan Stindt, Sandra Freier, Anja Sammt, Kristina Schoonjans, Philipp A. Lang, Sascha Kahlfuß, et al."CD11b Monoclonal Antibody (M1/70), APC-eFluor™ 780, eBioscience",Thermo Fisher Scientific,"Cat# 47-0112-82, RRID:AB_1603193" CLEC4F/CLECSF13,R&D Systems /Biotechne,Cat# AF2784; RRID:AB_2081339 c-Myc,Cell signaling Technology,13987 Cy3-AffiniPure Donkey Anti-Rabbit IgG (H+L),Jackson ImmunoResearch

(5) A comprehensive molecular atlas of the mesenchymal cell types in the mouse liverEMBO ReportsSeptember 15, 2025Riikka Pietilä, Guillem Genové, Giuseppe Mocci, Yuyang Miao, Jianping Liu, Stefanos Leptidis, Francesca Del Gaudio, Martin Uhrbom, Elisa Vázquez-Liébanas, Sonja Gustafsson, et al.(rabbit),Cell Signaling Technology,97778T CD68-APC (rat),BioLegend,137008 CLEC4F (goat),R&D Systems,AF2784 CLEC4F-AlexaFluor647 (mouse),BioLegend,156803 CNN1 (rabbit),Abcam,ab216651 EMCN (rat),eBiosciences,14-5851-82 EPCAM-APC

(6) Divergent Hepatic and Adipose Tissue Effects of Kupffer Cell Depletion in a Male Rat Model of Metabolic-Associated SteatohepatitisBiologyAugust 15, 2025Wiszniewski M, Mori D, Sanchez Puch SI, Martinez Calejman C, Cymeryng CB, Repetto EM.sections, as described previously [21]. The antibodies used in this study were as follows: CLEC4f 1:20, AF2784, and 3-nitrotyrosine (1:50, MAB3248) (R&D systems, Minneapolis, MN, USA). TRICT donkey anti-goat IgG

(7) Myeloid TGF-beta signaling shapes liver macrophage heterogeneity and metabolic liver disease pathogenesisJHEP ReportsJune 19, 2025Ziyi Meng, Xiaoxue Qiu, Zhimin Chen, Yu-tung Lee, Linkang Zhou, You Lu, Tongyu Liu, Siming Li, Benjamin Levi, Katherine A. Gallagher, et al.stained with primary antibodies against C-type lectin domain family 4 member F (CLEC4F) (R&D Systems, AF2784) or GPNMB (R&D systems, AF2330) and incubated at 4 °C overnight, before being stained with Alexa Fluor-conjugated

(8) An Nrf2-NF-κB Crosstalk Controls Hepatocyte Proliferation in the Normal and Injured LiverCellular and Molecular Gastroenterology and HepatologyFebruary 17, 2025Andrii Kuklin, Coenraad Frederik Slabber, Luigi Tortola, Chan Lap Kwan, Gerhard Liebisch, Vangelis Kondylis, Florian Mair, Manfred Kopf, Achim Weber, Sabine WernerTween 20",IHC p-Histone H3 (S10),9701S,Cell Signaling,1:200,"1% BSA in PBS, 0.1% Tween 20",IHC Clec4f,AF2784,R&D Systems,1:80,"1% BSA in PBS, 0.3% Triton X-100",IF Goat anti-rabbit biotin-conjugated antibodies,111-065-003,Jackson ImmunoResearch

(9) GDF2 and BMP10 coordinate liver cellular crosstalk to maintain liver healtheLifeOctober 25, 2024Zhao D, Huang Z, Li X, Wang H, Hou Q, Wang Y, Yan F, Yang W, Liu D, Yi S, et al.(eBioV.7C7 (V.7C7), eBioscience, 14-5851-81), anti-LYVE1 (AF2125-SP, R&D Systems), and anti-Clec4F (AF2784, R&D Systems). Fluorescent-conjugated secondary antibodies were purchased from Jackson ImmunoResearch

(10) Loss of embryonically-derived Kupffer cells during hypercholesterolemia accelerates atherosclerosis developmentNature communicationsSeptember 27, 2024Fima R, Dussaud S, Benbida C, Blanchet M, Lanthiez F, Poupel L, Brambilla C, Gélineau A, Dessena M, Blanc M, et al.#127648, BioLegend), VSIG4 (NLA14, #25-5752-82, ThermoFisher Scientific), CLEC4F (Polyclonal Goat IgG, #AF2784, R&D systems), TIMD4 (RMT4-54, #130008, BioLegend), CLEC2 (17D9, #146104, BioLegend), KI-67 (B56, #561277,

(11) Knockdown of Hyaluronan synthase 2 suppresses liver fibrosis in mice via induction of transcriptomic changes similar to 4MU treatmentScientific reportsFebruary 2, 2024Halimani N, Nesterchuk M, Tsitrina AA, Sabirov M, Andreichenko IN, Dashenkova NO, Petrova E, Kulikov AM, Zatsepin TS, Romanov RA, et al.optical section step. The following primary and secondary antibodies were used: Primary Ab: CLEC4F (AF2784, R&D Systems, 1:300); Desmin (ab32362, Abcam, 1:300); α-SMA (701457, Invitrogen, 1;100). Secondary Ab:

(12) Inhibition of CCl4-induced liver inflammation and fibrosis by a NEU3 inhibitorPloS oneJanuary 1, 2024Pilling D, Martinez TC, Gomer RH.clone SP7, NB600-1441, Novus Biologicals, Centennial, CO, USA) to detect T-cells, anti-CLEC4F (goat Ab, AF2784, Novus Biologicals) to specifically detect Kupffer cells, anti-MRP8 (goat Ab, AF3059, Novus Biologicals)

(13) Long-Term L-Glutamine Treatment Reduces Hemolysis without Ameliorating Hepatic Vaso-Occlusion and Liver Fibrosis in a Mouse Model of Sickle Cell DiseaseBiomedicinesAugust 29, 2023Katoch O, Ungalara R, Kaminski T, Li Z, Dubey RK, Burholt I, Gudapati S, Pradhan-Sundd T.overnight. The primary antibodies used include: F4/80 (CST, 70076S, 0.435 μg/mL), CLEF4C (R&D Systems, AF2784, 0.025 μg/mL) and (abcam, AB75973, 0.07 µg/mL). The secondary antibodies used include anti-Mouse/Rabbit

(14) Roquin-1 resolves sepsis-associated acute liver injury by regulating inflammatory profiles via miRNA cargo in extracellular vesiclesiScienceAugust 18, 2023Zheng L, Ling W, Zhu D, Li Z, Li Y, Zhou H, Kong L.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Roquin-1,Invitrogen,PA5-34519 CD11b,Novus,NB110-89474 F4/80,Novus,NB600-404 CX3CR1,Novus,NBP1-76949 Clec4F,Novus,AF2784 TSC22D2,Novus,NBP2-46238 Ago2,Abcam,186733 p-Ago2,Abcam,215746 Dcp1a,Abcam,240275 CD63,Abcam,217345 Tsg101,Abcam,125011 Flot1,Abcam,133497 Rab7,Abcam,137029 GW182,Abcam,70522 HSP70,Abcam,2787 Dicer,Abcam,14601 STAT3,Abcam,68153 p-STAT3,Abcam,267373 Flag,Abcam,205606 AMPKα,Abcam,32047 p-AMPKα,Abcam,210714 mTOR,Abcam,134903 p-mTOR,Abcam,109268 anti-CD11c

(15) Myeloid-specific deletion of chitinase-3-like 1 protein ameliorates murine diet-induced steatohepatitis progressionJournal of molecular medicine (Berlin, Germany)July 1, 2023Kim AD, Kui L, Kaufmann B, Kim SE, Leszczynska A, Feldstein AE.anti-F4/80 (Cat 123,106, 1:100, BioLegend) and anti-Ly6C (ab15627, 1:100, Abcam), and goat anti-CLEC4F (AF2784, 1:100, R&D Systems) antibodies diluted in Dako Antibody Diluent. After overnight incubation, cells

(16) Kupffer-cell-derived IL-6 is repurposed for hepatocyte dedifferentiation via activating progenitor genes from injury-specific enhancersCell stem cellMarch 2, 2023Li L, Cui L, Lin P, Liu Z, Bao S, Ma X, Nan H, Zhu W, Cen J, Mao Y, et al.

(17) Hepatocyte-derived DPP4 regulates portal GLP-1 bioactivity, modulates glucose production, and when absent influences NAFLD progressionJCI insightJanuary 24, 2023Trzaskalski NA, Vulesevic B, Nguyen MA, Jeraj N, Fadzeyeva E, Morrow NM, Locatelli CA, Travis N, Hanson AA, Nunes JR, et al.Thermo Fisher Scientific, clone CI:A3-1, catalog MA1-91124, 1:250), and CLEC4F (R&D Systems, Bio-Techne, catalog AF2784, 1:200) were incubated overnight at 4°C. Slides were washed with PBS-Tween, then incubated with secondary

(18) Spatial proteogenomics reveals distinct and evolutionarily conserved hepatic macrophage nichesCellJanuary 20, 2022Guilliams M, Bonnardel J, Haest B, Vanderborght B, Wagner C, Remmerie A, Bujko A, Martens L, Thoné T, Browaeys R, et al.(G8.8),eBioscience,17-5791-82; RRID: AB_2716944 Goat Polyclonal Clec4F-Unconjugated,R & D Systems,AF2784; RRID: AB_2081339 Rat Monoclonal Clec4F-Unconjugated (370901),R & D Systems,MAB2784; RRID: AB_2081338 Goat

(19) NLRP6 Inflammasome Modulates Disease Progression in a Chronic-Plus-Binge Mouse Model of Alcoholic Liver DiseaseCellsJanuary 6, 2022Mainz RE, Albers S, Haque M, Sonntag R, Treichel NS, Clavel T, Latz E, Schneider KM, Trautwein C, Otto T.BD Biosciences, Franklin Lakes, NJ, USA; MUC2: 1:300, #ab76774, Abcam, Cambridge, UK; CLEC4F: 1:200, #AF2784, R&D Systems, Minneapolis, MN, USA) overnight at 4 °C. After washing, the sections were incubated with

(20) COMMD10 is critical for Kupffer cell survival and controls Ly6C^hi monocyte differentiation and inflammation in the injured liverCell reportsNovember 16, 2021Cohen K, Mouhadeb O, Ben Shlomo S, Langer M, Neumann A, Erez N, Moshkovits I, Pelet R, Kedar DJ, Brazowski E, et al.(NLA14),ThermoFisher Scientific,Cat#25–5752-82; RRID: AB_2637431 Unconjugated anti mouse CLEC4F,R&D Systems,Cat#AF2784; RRID: AB_2081339 PE/Cy7 anti mouse CD64 (X54–5/7.1),BioLegend,Cat#139314; RRID: AB_2563904 PE anti

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CLEC4F/CLECSF13 Antibody

CLEC4F/CLECSF13 Antibody

CLEC4F/CLECSF13 Antibody

Mouse CLEC4F/CLECSF13 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (35)

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Mouse CLEC4F/CLECSF13 Antibody from R&D Systems, a Bio-Techne Brand

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