Western blot analysis of extracts (35ug) from different cell lines and tissues by using anti-RAD52 rabbit polyclonal antibody.

Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and RAD52-Knockout HeLa cells (KO, Cat# LC830600) were separated by SDS-PAGE and immunoblotted with anti-RAD52 rabbit polyclonal antibody TA890144 (1:1000`). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.