HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SMAD7 (Cat# RC210400, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMAD7 antibody (Cat# TA890026). Positive lysates [LY416991] (100ug) and [LC416991] (20ug) can be purchased separately from OriGene.