HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF4 (RC207273, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF4 (1:2000). Positive lysates LY432659 (100ug) and LC432659 (20ug) can be purchased separately from OriGene.