HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CEBPA (Cat# RC218955, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CEBPA antibody (Cat# TA890066). Positive lysates [LY418038] (100ug) and [LC418038] (20ug) can be purchased separately from OriGene.