HDAC3 Antibody PA1-862 from Thermo Fisher Scientific | Biocompare.com

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ICC/IF

Immunofluorescent analysis of HDAC3 in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a HDAC3 polyclonal antibody (Product # PA1-862) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552). HDAC3 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
ICC/IF

Immunofluorescent analysis of HDAC3 in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a HDAC3 polyclonal antibody (Product # PA1-862) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552). HDAC3 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

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Supplier Page from
Thermo Fisher Scientific for
HDAC3 Antibody

The HDAC3 Antibody from Thermo Fisher Scientific is a Rabbit Polyclonal antibody to HDAC3, and Histone Deacetylase 3. This antibody recognizes Homo Sapiens, Human, Rat, Canine, and Hamster antigen. The HDAC3 Antibody has been shown to work in the following applications: Assay, ChIP, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry - fixed, Immunoprecipitation, and Western Blot.

Product Specs
ItemHDAC3 Antibody
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberPA1-862
NCBI Gene AliasesHD3, KDAC3, RPD3-2, RPD3
ReactivityCanine, Hamster, Human, Rat
FormatPBS with 1mg/mL BSA and 0.05% sodium azide
TargetHDAC3
HostRabbit
Quantity100 µg
ApplicationsChIP assay, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Conjugate/TagUnconjugated
ClonalityPolyclonal
NCBI Full Gene Namehistone deacetylase 3
Concentration1 mg/mL
ImmunogenSynthetic peptide corresponding to residues C D(415) G D H D N D K E S D V E I(428) of human HDAC3
IsotypeIgG
Storage Temperature-20° C, Avoid Freeze/Thaw Cycles
PurityAntigen affinity chromatography
Antibody TypePrimary
Storage-20°C, Avoid Freeze/Thaw Cycles

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Thermo Fisher Scientific

Thermo Fisher Scientific

Thermo Fisher Scientific

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Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

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Website: www.thermofisher.com

(1) Regulated clearance of histone deacetylase 3 protects independent formation of nuclear receptor corepressor complexesThe Journal of biological chemistryApril 6, 2012Guo C, Gow CH, Li Y, Gardner A, Khan S, Zhang J.

(2) The transcriptional repressor Sp3 is associated with CK2-phosphorylated histone deacetylase 2The Journal of biological chemistrySeptember 27, 2002Sun JM, Chen HY, Moniwa M, Litchfield DW, Seto E, Davie JR.

(3) Differential display cloning of a novel human histone deacetylase (HDAC3) cDNA from PHA-activated immune cellsBiochemical and biophysical research communicationsJanuary 26, 1998Dangond F, Hafler DA, Tong JK, Randall J, Kojima R, Utku N, Gullans SR.

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