WB: The whole cell lysate derived from human umbilical vein endothelial cell was stimulated by VEGF-A for 20 min, then immunoprecipitated by Rabbit anti-VEGFR2 followed by immuneprobing with Rabbit anti-VEGFR-2 at 1:500. The immunoreactive bands are observed around ~200kDa (non-phospho form) and ~ (Phospho-form) (lane 1). The lane 2 is a negative control.