HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGF2 (RC217426, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGF2 rab. Positive lysates LY400733 (100ug) and LC400733 (20ug) can be purchased separately from OriGene.