Rabbit IgG Isotype Control (Alexa Fluor ® 647 Conjugate) 3452S from Cell Signaling Technology

Product Specs
ItemRabbit IgG Isotype Control (Alexa Fluor ® 647 Conjugate)
CompanyCell Signaling Technology
Price $149.00 Inquire
Catalog Number3452S
Quantity100 µl
ApplicationsFC-FP, FC-L
HostRabbit
Conjugate/TagAlexa Fluor ® 647
ClonalityPolyclonal
Research AreaRelated Products

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) The mitochondrial unfolded protein response and metabolic reprogramming promote PASMC proliferation in response to sphingosine kinase-1/sphingosine-1-phosphate signalingbioRxivJune 14, 2025Angelia D. Lockett, Aaron Snow, Pontian Adogamhe, Justin Sysol, Manas Yadav, Tendo Mubuuke, Marta T. Gomes, Todd Cook, Amanda Fisher, Micheala A. Aldred, et al.Cell Signaling Biotechnology (Danvers, MA): HSP60 (12165s), ClpP (14181s), eIF2α (2103s), PCNA (13110s), p-Drp1 (3452s/3455s), Drp1 (5391s), mitofusin2 (11925s) anti-mouse-HRP (7076s), anti-rabbit-HRP (7074s), and Lamin-B2-HRP

(2) Remission induction in refractory, drug resistant pediatric PICALM::MLLT10+ B-cell acute lymphoblastic leukemia by venetoclaxLeukemiaApril 15, 2025Alexandra Niedermayer, Jana Stursberg, Anke Katharina Bergmann, Martin Zimmermann, Gunnar Cario, Monika Brüggemann, Rolf Köhler, Daniel Steinbach, Christian Reimann, Felix Seyfried, et al.IgG1 Isotype Control (Alexa Fluor 488, #4878, CST) and rabbit IgG Isotype Control (Alexa Fluor 647, #3452, CST) in triplicates. Representative FACS plots are shown and mean fluorescence intensity ratios (MFIR)

(3) Circular RNA circSTX12 regulates osteo-adipogenic balance and proliferation of BMSCs in senile osteoporosisCell Mol Life SciApril 7, 2025Huimin Gu, Wenhui Yu, Pei Feng, Chenying Zeng, Qian Cao, Fenglei Chen, Ziming Wang, Huiyong Shen, Yanfeng Wu, Shan Wang(Abcam, ab32027, 1:1000), MST1 (CST, bs-8477R, 1:1000), or their IgG controls (Cell Signaling Technology, 3452 or 37988) at 4 °C overnight. Then, protein-G agarose beads (40 L, Beyotime Biotechnology) were added,

(4) WAC Facilitates Mitophagy‐mediated MSC Osteogenesis and New Bone Formation via Protecting PINK1 from Ubiquitination‐Dependent DegradationAdvanced ScienceNovember 18, 2024Shuai Fan, Jinteng Li, Guan Zheng, Ziyue Ma, Xiaoshuai Peng, Zhongyu Xie, Wenjie Liu, Wenhui Yu, Jiajie Lin, Zepeng Su, et al.Technology, Cat. No. 205 606), or their corresponding IgG controls (Cell Signaling Technology, Cat. No. 3452) were subsequently co‐incubated overnight. The subsequent day, beads were retrieved and washed. After

(5) ASC specks as a single-molecule fluid biomarker of inflammation in neurodegenerative diseasesNature CommunicationsNovember 11, 2024Evgeniia Lobanova, Yu P. Zhang, Derya Emin, Jack Brelstaff, Lakmini Kahanawita, Maura Malpetti, Annelies Quaegebeur, Kathy Triantafilou, Martha Triantafilou, Henrik Zetterberg, et al.Alexa 647 Mouse IgG1 (Cat. No. MA5-18168; PRID: AB_2539542, Invitrogen), Alexa 647 Rabbit IgG (Cat. No. 3452S; RRID: AB_10695811, Cell Signalling Technology), Bovine Serum Albumin (B9000S, New England biolabs)

(6) An unbiased proteomic analysis of PAD4 in human monocytes: novel substrates, binding partners and subcellular localizationsPhilosophical Transactions of the Royal Society B: Biological SciencesNovember 20, 2023Mekha A. Thomas, Seok-Young Kim, Ashley M. Curran, Barbara Smith, Brendan Antiochos, Chan Hyun Na, Erika Darrahantibody labelling kit (Invitrogen) and used at 0.01 µg µl−1. Equal amounts of AF647-labelled rabbit IgG (3452S, Cell Signaling Technology) served as isotype control. Cells were incubated with labelled antibodies

(7) Molecular and epigenetic alterations in normal and malignant myelopoiesis in human leukemia 60 (HL60) promyelocytic cell line modelFrontiers in Cell and Developmental BiologyFebruary 2, 2023Jhinuk Basu, Swati Madhulika, Krushna Chandra Murmu, Smrutishree Mohanty, Priyanka Samal, Asima Das, Soumendu Mahapatra, Subha Saha, Indranil Sinha, Punit Prasad(CST-12064S), H3K27me3-Alexa Fluor 488 (CST-5499S) and the respective isotype controls Alexa Fluor 647 (CST-3452S) and Alexa Fluor 488 (CST-4340S) using 1X permeabilization buffer (Thermo—00833356). All flow cytometry

(8) Distinct Cell Adhesion Signature Defines Glioblastoma Myeloid-Derived Suppressor Cell SubsetsCancer researchNovember 15, 2022Bayik D, Bartels CF, Lovrenert K, Watson DC, Zhang D, Kay K, Lee J, Lauko A, Johnson S, Lo A, et al.Cell Signaling Technology, 4375S), or rabbit IgG Isotype Control (Alexa Fluor 647 Conjugate; Cell Signaling Technology, 3452S) in 1× eBioscience Foxp3/transcription factor permeabilization buffer for 1 hour at room temperature.

(9) Oncogenic BRAF induces whole-genome doubling through suppression of cytokinesisNature communicationsJuly 15, 2022Darp R, Vittoria MA, Ganem NJ, Ceol CJ.for flow cytometry were: CYCLIN D1, 1:100 (NBP2-33138AF647, Novus Bio), Rabbit IgG Isotype control, 1:100 (3452S, Cell Signaling), HA tag, 1:100 (IC6875R, R&D systems), Mouse IgG1 Isotype control 1:100 (IC002R, R&D systems

(10) Adenosine Monophosphate Activated Protein Kinase (AMPK) enhances chemotherapy response in Acute Myeloid Leukemia (AML)Cancer LettersJune 1, 2022Lais Ghiraldeli, Rebecca Anderson, Kristin Pladna, Timothy S. Pardee1:50) according to manufacturer’s protocol. Rabbit IgG was used as an isotype control (Cell signaling, #3452, 1:50). Samples were analyzed by flow cytometry. 2.9 Mitochondrial Respiration Assays: All oxygen

(11) The mitochondrial iron transporter ABCB7 is required for B cell development, proliferation, and class switch recombination in miceeLifeNovember 11, 2021Lehrke MJ, Shapiro MJ, Rajcula MJ, Kennedy MM, McCue SA, Medina KL, Shapiro VS.interest) Antibody,Rabbit IgG Isotype Control AF647 (Rabbit monoclonal),Cell Signaling Technology,Cat#:3452; RRID:AB_10695811,FC (concentration matched antibodies of interest) Antibody,"Rabbit IgG Isotype Control

(12) SMAD-specific E3 ubiquitin ligase 2 promotes angiogenesis by facilitating PTX3 degradation in MSCs from patients with ankylosing spondylitisStem CellsMay 1, 2021Ma M, Yang W, Cai Z, Wang P, Li H, Mi R, Jiang Y, Xie Z, Sui P, Wu Y, et al.(12024; CST), PTX3 (373951; Santa Cruz Bio.), Flag (8146; CST), Myc (2276; CST), or their IgG controls (3452, 37988; CST) for 2 hours, followed by Protein G‐agarose beads (10004D; Thermo Fisher Scientific, Rockford,

(13) NEDD4 Induces K48-Linked Degradative Ubiquitination of Hepatitis B Virus X Protein and Inhibits HBV-Associated HCC ProgressionFrontiers in OncologyMarch 9, 2021Tao Wan, Zhao Lei, Biao Tu, Tianyin Wang, Jiale Wang, Feizhou HuangFlag-Tag (4 µl, Cell Signaling Technology, 14793) or their IgG control (Cell Signaling Technology, 3452 or 37988) at 4°C overnight. Then, protein-G agarose beads (40 L, Beyotime Biotechnology) were added,

(14) Dysregulated NF-κB-Dependent ICOSL Expression in Human Dendritic Cell Vaccines Impairs T-cell Responses in Patients with MelanomaCancer immunology researchDecember 1, 2020Maurer DM, Adamik J, Santos PM, Shi J, Shurin MR, Kirkwood JM, Storkus WJ, Butterfield LH.Conjugate; Cell Signaling #5733S), Rabbit IgG Isotype Control (Alexa Fluor® 647 Conjugate; Cell Signaling # 3452S), Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate; Cell Signaling #5742). The following antibodies

(15) Pharmacologic Suppression of B7-H4 Glycosylation Restores Antitumor Immunity in Immune-Cold Breast CancersCancer discoveryDecember 1, 2020Song X, Zhou Z, Li H, Xue Y, Lu X, Bahar I, Kepp O, Hung MC, Kroemer G, Wan Y.Conjugate) (Cell Signaling Technology, 4414), Rabbit IgG Isotype Control (Cell signaling Technology, 3452), (Alexa Fluor(R) 488 Conjugate) (Cell Signaling Technology, 4340S), Anti-rabbit IgG (H+L), F(ab’)2

(16) Bronchioalveolar stem cells derived from mouse-induced pluripotent stem cells promote airway epithelium regenerationStem cell research & therapyOctober 2, 2020Kawakita N, Toba H, Miyoshi K, Sakamoto S, Matsumoto D, Takashima M, Aoyama M, Inoue S, Morimoto M, Nishino T, et al.(#4878S; Cell Signaling Technology, Danvers, MA) and rabbit IgG conjugated with Alexa Fluor® 647 (#3452S; Cell Signaling Technology) were used as isotype controls. After incubation with the antibodies for

(17) TRAF4 acts as a fate checkpoint to regulate the adipogenic differentiation of MSCs by activating PKM2EBioMedicineApril 1, 2020Cen S, Li J, Cai Z, Pan Y, Sun Z, Li Z, Ye G, Zheng G, Li M, Liu W, et al.(14793, Cell Signaling Technology), Myc-Tag (2276, Cell Signaling Technology), and their IgG control (3452 or 37988, Cell Signaling Technology). The immunoprecipitates were collected, washed, and boiled in Laemmli

(18) Proteome Instability Is a Therapeutic Vulnerability in Mismatch Repair-Deficient CancerCancer cellMarch 16, 2020McGrail DJ, Garnett J, Yin J, Dai H, Shih DJH, Lam TNA, Li Y, Sun C, Li Y, Schmandt R, et al.lgG-AlexaFluor648 (EPR25A),Abeam,Cat# ab199093; RRID: AB 2818935 Rabbit IgG Control (Poly),Cell Signaling,Cat# 3452; RRID: AB 10695811 FITC B2M (B2M-01),ThermoFisher,Cat# A15737; RRID: AB 10762969 PE-Cy7 HLA-ABC (W6/32),ThermoFisher,Cat#

(19) TRAF4 positively regulates the osteogenic differentiation of mesenchymal stem cells by acting as an E3 ubiquitin ligase to degrade Smurf2Cell death and differentiationDecember 1, 2019Li J, Wang P, Xie Z, Wang S, Cen S, Li M, Liu W, Tang S, Ye G, Zheng G, et al.Myc-Tag (1:1000, Cell Signaling Technology, 2276), or their IgG control (Cell Signaling Technology, 3452 or 37988) at 4 °C overnight. Then, protein-G agarose beads (40 L, Beyotime Biotechnology) were added,

(20) Discovery of Covalent CDK14 Inhibitors with Pan-TAIRE Family SpecificityCell chemical biologyJune 20, 2019Ferguson FM, Doctor ZM, Ficarro SB, Browne CM, Marto JA, Johnson JL, Yaron TM, Cantley LC, Kim ND, Sim T, et al.T199,Cell Signaling Technologies,Cat # 3451 Rabbit polyclonal anti NPM,Cell Signaling Technologies,Cat # 3452 Rabbit monoclonal anti Phospho-RB S608,Cell Signaling Technologies,Cat # 8147 Rabbit monoclonal anti

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Rabbit IgG Isotype Control (Alexa Fluor ® 647 Conjugate)

Rabbit IgG Isotype Control (Alexa Fluor ® 647 Conjugate) from Cell Signaling Technology

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Cell Signaling Technology

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Rabbit IgG Isotype Control (Alexa Fluor ® 647 Conjugate) from Cell Signaling Technology

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