HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ALDH9A1 (Cat# RC216921, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDH9A1 antibody (Cat# TA890045). Positive lysates [LY424566] (100ug) and [LC424566] (20ug) can be purchased separately from OriGene.