SRF (D71A9) XP ® Rabbit mAb 5147S from Cell Signaling Technology

Product Specs
ItemSRF (D71A9) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $371.00 Inquire
Catalog Number5147S
Quantity100 µl
ApplicationsW, IP, ChIP
ReactivityH, M, R, Mk, Pg
TargetSRF
HostRabbit
NCBI Gene AliasesMCM1
ClonalityMonoclonal
NCBI Full Gene Nameserum response factor
Research AreaMAP Kinase Signaling
IsotypeIgG
CloneD71A9
Molecular Weight67

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) RSK1–SRF Signaling Axis Drives Fibroblast Activation and Pulmonary Fibrosis: Genetic Causality and Therapeutic TargetingiScienceMay 15, 2026Tianzheng Lan, Hui Gao, Yuanyuan Chen, Yi Liu, Chaojie Wang, Chaowen Shi,Cell Signaling Technology,Cat#4261; RRID: AB_2239783 Rabbit monoclonal anti-SRF,Cell Signaling Technology,Cat#5147; RRID: AB_10694554 Rabbit monoclonal anti-Phospho-RSK1 (Thr359/Ser363),Cell Signaling Technology,Cat#9344;

(2) HDL Regulates TGFβ-Receptor Lipid Raft Partitioning, Restoring Contractile Features of Cholesterol-Loaded Vascular Smooth Muscle CellsJACC: Basic to Translational ScienceMarch 1, 2026Prashanth Thevkar Nagesh, Shruti Rawal, Hitoo Nishi, Tarik Zahr, Joseph M. Miano, Mary Sorci-Thomas, Hao Xu, Naveed Akbar, Robin P. Choudhury, Mark W. Feinberg, et al.Health). The primary antibodies used were as follows: ACTA2 (#A2547, Sigma); CNN1 (#M3556, DAKO); SRF (#5147, Cell Signaling); p38MAPK (#sc-535, Santa Cruz Biotechnology); SMAD2/3 (#8685, Cell Signaling); TUBA

(3) Ionic Regulation of Mechanosurveillance and Metastasis via the MRTFA/KCNMB1 AxisbioRxivJanuary 16, 2026Alexa M Gajda, Mohamed Haloul, Vinay Pai, Keyvan Mollaeian, Khushi J Patel, Raymundo Rodriguez-Lopez, Katie M Beverley, Mark A Sanborn, Kihak Lee, Caitlyn C Castillo, et al.Cell Signaling Technology), Normal Rabbit IgG (Cat#. 2729, Cell Signaling Technology), SRF (D71A9) XP Rabbit mAb (#5147, Cell Signaling Technology), and MKL1/MRTF-A (E2V2I) Rabbit mAb (Cat#. 97109, Cell signaling Technology)

(4) MAPK14 converges on key transcriptional machinery to promote vascular smooth muscle cell degeneration in abdominal aortic aneurysmSignal Transduction and Targeted TherapyJanuary 12, 2026Xiaoliang Wu, Chunhui Wang, Nestor Ishimwe, Wei Zhang, Jaser Doja, Shengshuai Shan, Chunyu Ge, Yong Sun, Jinjing Zhao, Micah Castillo, et al.Cat #20--153), and incubated overnight at 4 °C with an anti-SRF antibody (Cell Signaling Technology, 5147S) or a species-matched IgG control (Cell Signaling Technology, 2729S). After incubation with Dynabeads

(5) YK-4-279 Induces Osteosarcoma Cell Cycle Arrest, DNA Damage Response, and Apoptosis by Regulating the MAPK CascadeDrug Des Devel TherDecember 26, 2025Weifeng Wang, Yuli Zhang, Xiuqin Jia, Liren Han, Ming Xin(Erk1/2)(Thr202/Tyr204)(4370s, 1:1000), Phospho-SAPK/JNK (Thr183/Tyr185) (9255s, 1:1000), β-actin (Ms)(3700s, 1:1000), GAPDH(Rb)(5147s, 1:1000), purchased from Cell Signaling Technology (Danvers, MA, USA). Additional antibodies included

(6) Early-phase impact of obesity-associated stress on murine vascular smooth muscle cells depends on EGFR and sexCommun BiolDecember 22, 2025Virginie Dubourg, Sindy Rabe, Narjes Nasiri-Ansari, Michael Kopf, Sigrid Mildenberger, Gerald Schwerdt, Barbara Schreier, Michael Geklepermeabilization buffer. Primary antibodies (from Cell Signaling Technologies, Frankfurt, Germany: SRF #5147, 1:2000; phospho-SRFS103 #4261, 1:1000; ELK-1 #9182, 1:500; phospho-ELK1S383 #9186, 1:500; MRTF-A #14760,

(7) Hypoglycemia induces brain metabolic reprogramming and neurodegeneration via serum response factor and myocardin-related transcription factor-ASignal Transduct Target TherDecember 17, 2025Minjeong Jang, Hyung Jin Choi, Hae-June Lee, Hong Nam Kimanti-ß-amyloid (8243; Cell Signaling Technology, Danvers, MA, USA) for mouse analysis), anti-SRF (#5147, Cell Signaling Technology), anti-MRTF-A (sc-390324, Santa Cruz Biotechnology Inc., Santa Cruz, CA,

(8) Functional requirements of the liver isoform of phosphofructokinase-1 in breast cancer cell migrationJournal of Cell ScienceDecember 1, 2025Heather L. Hansen, Bradley A. Webb(Thermo Fisher Scientific, Rockfield IL, USA; 44-609G; 1:1000) or GAPDH (Cell Signaling Technologies, Danvers MA, USA; 5147; 1:2000). The primary antibody was removed, and membranes were washed for 5 min with TBST three times.

(9) Mouse scalp development requires Rac1 and SRF for the maintenance of mechanoresponsive mesenchymeDevelopmentNovember 15, 2025Bharath H. Rathnakar, Alex Rackley, Hae Ryong Kwon, Jang H. Kim, William L. Berry, Lorin E. Olsonwith primary antibodies (anti-mouse Rac1 CST (Cell Signaling Technology) #8631, anti-rabbit SRF CST#5147, anti-mouse LaminA/C CST#4777, anti-rabbit β-Tubulin CST#2146S, anti-rabbit α-SMA CST#19245, anti-rabbit

(10) A smooth muscle cell lncRNA controls angiogenesis in chronic limb-threatening ischemia through miR-143-3p/HHIP signalingJournal of Clinical InvestigationOctober 15, 2025Ming Zhai, Anurag Jamaiyar, Jun Qian, Winona W. Wu, Emre Bektik, Vinay Randhawa, Camila De Oliveira Vaz, Arvind K. Pandey, Akm Khyrul Wara, Madhur Sachan, et al.Cells were frozen at –80°C for at least a day to improve cell lysis. Antibodies for HHIP, SRF (CST, 5147) or IgG isotype control antibodies (Millipore, PP64B) were conjugated to Pierce Protein A/G magnetic beads

(11) Poly(ADP-ribose) polymerase 1 orchestrates vascular smooth muscle cell homeostasis in arterial diseaseExperimental & molecular medicineAugust 1, 2025Xu W, Wu Y, Mao R, Jia Y, Jiang H, Zhang F, Huang D, He X, Wang C, Huang K.were from Abcam. Antibodies for FLAG (14793, 1:1,000), His (12698, 1:1,000), HA (3724, 1:1,000), SRF (5147, 1:1,000), c-Jun (9165, 1:1,000) and NF-κB p65 (8242, 1:1,000) were from Cell Signaling Technology.

(12) Declining activity of serum response factor in aging aorta in relation to aneurysm progressionJournal of Biological ChemistryMarch 12, 2025Catarina Rippe, Joakim Armstrong Bastrup, Johan Holmberg, Katarzyna Kawka, Marycarmen Arévalo Arévalo Martinez, Sebastian Albinsson, Thomas A. Jepps, Karl SwärdMcam (Sigma-Aldrich, SAB5600062), Lmod1 (Proteintech, 15117-1-AP), Srf (Cell Signaling Technology, #5147), Cavin1 (Abcam, ab48824), Cavin3 (Proteintech, 16250-1-AP), Actg2 (St John's Laboratory, STJ91463),

(13) Functional screen identifies RBM42 as a mediator of oncogenic mRNA translation specificityNature Cell BiologyMarch 1, 2025Joanna R. Kovalski, Goksu Sarioglu, Vishvak Subramanyam, Grace Hernandez, Gilles Rademaker, Juan A. Oses-Prieto, Macey Slota, Nimmy Mohan, Kaylee Yiakis, Isabelle Liu, et al.(4267, Cell Signaling, 1:1000), anti-c-Jun (Cell Signaling, 9165, 1:1000), anti-SRF (Cell Signaling, 5147, 1:1000), anti-PAK4 (Bethyl, A300–356A, 1:1000), anti-EZR (Cell Signaling, 3145, 1:1000), anti-RPS6

(14) Decoding the m6A epitranscriptomic landscape for biotechnological applications using a direct RNA sequencing approachNat CommunJanuary 18, 2025Chuwei Liu, Heng Liang, Arabella H. Wan, Min Xiao, Lei Sun, Yiling Yu, Shijia Yan, Yuan Deng, Ruonian Liu, Juan Fang, et al.(1:1000, A11684, ABclonal), rabbit monoclonal anti-FTO (1:1000, A3861, ABclonal), and anti-GAPDH (1:1000, 5147, Cell Signaling Technologies). ATP and NAD + /NADH assay The levels of ATP and NAD + /NADH were measured

(15) Extracts of Drynariae Rhizoma Promote Bone Formation in OVX Rats through Modulating the Gut MicrobiotaPlanta MedicaJanuary 17, 2025Qing Lin, Xinchen Ouyang, Qi Pan, Jiajia Huang, Zhifen Zhang, Yumei Yang, Haoyu Wang, Li Yang, Xiaofeng Zhu, Xiaoyun Li, et al.Technology), anti-RUNX2 (ab192256; Abcam), and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 5147S; Cell Signaling Technology), all at a dilution of 1 : 1000 and at 4 °C overnight. The next day, the

(16) Single-molecule analysis reveals that IPMK enhances the DNA-binding activity of the transcription factor SRFNucleic acids researchJanuary 7, 2025Ahn H, Yu J, Ryu K, Ryu J, Kim S, Park JY, Kim JK, Jung I, An H, Hong S, et al.2622S), Flag (Sigma Aldrich, F1804), α-tubulin (Sigma Aldrich, T5168), SRF (Cell Signaling Technology, 5147), IPMK (24), c-Fos (Santa Cruz biotechnology, sc-7202), SNAP (Invitrogen, CAB4255)] diluted in 5% bovine serum albumin (BSA

(17) Excess shed mesothelin disrupts pancreatic cancer cell clustering to impair peritoneal colonizationThe FASEB JournalDecember 14, 2024Theressa Ewa, Neel Panchwagh, Chin‐Hsien Tai, Leela Rani Avula, Sarah Joseph, Michael W. Rudloff, Nargis Malik, Xianyu Zhang, Christine AlewineGoat Anti‐Mouse IgG (H+L)‐HRP Conjugate (Bio‐Rad, #1706516); for GAPDH: GAPDH (Cell Signaling, Cat#:5147) and Goat Anti‐Rabbit IgG (H+L)‐HRP Conjugate (Bio‐Rad; #1706515). 2.8 Mouse experiments All animal experiments

(18) Ectopic USP15 expression inhibits HIV-1 transcription involving changes in YY1 deubiquitination and stabilityFrontiers in Cellular and Infection MicrobiologyNovember 18, 2024Sahar Rezaei, Khalid A. Timani, Ying Liu, Johnny J. HeSignaling Technology), YY1 rabbit mAb D5D9Z (#46395, Cell Signaling Technology), SRF rabbit mAb D71A9 (#5147, Cell Signaling Technology), goat anti-rabbit HRP-conjugated IgG, (#7074, Cell Signaling Technology)

(19) LncRNA GTL2 regulates myoblast proliferation and differentiation via the PKA-CREB pathway in Duolang sheepZoological ResearchNovember 18, 2024Qian Chen, 中国农业科学院北京畜牧兽医研究所, 北京 100193, 中国, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China, Jing-Jing Bao, He-Chun Zhang, Chang Huang, Qian Zhao, Ya-Bin Pu, Lin Jiang, Adel Hosseiny, et al.USA), MyoD (1:1 000, 18943-1-AP, Proteintech, China), MyoG (1:1 000, ab1835, Abcam, UK), SRF (1:1 000, 5147S, Cell Signaling Technology, USA), GAPDH (1:4 000, HRP-60004, Proteintech, China), and β-actin (1:4 000,

(20) Anti-fibrogenic effect of umbilical cord–derived mesenchymal stem cell–conditioned media in human esophageal fibroblastsScientific ReportsSeptember 27, 2024Yoon Jeong Choi, Jee Hyun Kim, Yeonju Lee, Hee Jang Pyeon, In Kyung Yoo, Jun Hwan YooSanta Cruz Biotechnology, Dallas, TX), GAPDH (#2118, Cell Signaling), Mkl1(MRTF-A) (21166-1-AP, ProteinTech), SRF (#5147, Cell Signaling), HDAC1 (#5356, Cell Signaling), phospho-YAP (Ser127) (#13008, Cell Signaling), YAP

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SRF (D71A9) XP ® Rabbit mAb

SRF (D71A9) XP ® Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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SRF (D71A9) XP ® Rabbit mAb from Cell Signaling Technology

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