eEF2k Antibody 3692S from Cell Signaling Technology

Product Specs
ItemeEF2k Antibody
CompanyCell Signaling Technology
Price $306.00 Inquire
Catalog Number3692S
Quantity100 µl
ApplicationsW, IP, IF-IC
ReactivityH, R, Mk
TargeteEF2K
HostRabbit
NCBI Gene AliasesCaMKIII, HSU93850, eEF-2K
NCBI Full Gene Nameeukaryotic elongation factor 2 kinase
ClonalityPolyclonal
Research AreaTranslational Control
Molecular Weight105

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Cell Signaling Technology

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Danvers, Massachusetts 01923

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) A dose-dependent switch in translation capacity controls the transcription factor response to H 2 O 2 stressbioRxivJanuary 31, 2026Chance Parkinson, Woody March-Steinman, Elizabeth Jose, Lisa Shanks, Andrew L. PaekAC-74 (Sigma) was used as a loading control (1:20,000). Anti-eEF2K rabbit pAb from Cell Signaling Cat# 3692S (1:1000). Anti-Tuberin/TSC2 (D93F12) XP® rabbit mAb from Cell Signaling Cat# 4308S (1:1000). Anti-4E-BP1 (53H11) rabbit mAb

(2) Overactive Neuronal eEF2K/eEF2 signaling is associated with cognitive impairment and apathy-like behaviorMolecular PsychiatryDecember 14, 2025Hannah M. Jester, Noelle Nicol, Qian Yang, Yankai Zhang, Amelia Suhocki, Xueyan Zhou, Ethan Underwood, Hadi Pourhadi, Dongmei Cheng, Jingyun Lee, et al.the following primary antibodies overnight at 4 °C: eEF2K (1:500, Cell Signaling Technology, catalog 3692), phospho-eEF2 (Thr56) (1:1000, Cell Signaling Technology, catalog 2331); eEF2 (1:1000, Cell Signaling Technology

(3) HMGA2 and protein leucine methylation drive pancreatic cancer lineage plasticityNat CommunMay 26, 2025Stephanie Dobersch, Naomi Yamamoto, Aidan Schutter, Sarah M. Cavender, Tess M. Robertson, Nithya Kartha, Annie N. Samraj, Ben Doron, Lisa A. Poole, Cynthia L. Wladyka, et al.Cell Signaling #9644), anti-pEIF2K (1:1000, Cell Signaling #3691) and anti-EIF2K (1:1000, Cell Signaling #3692). Western blots shown in the manuscript are representative experiments of two individual replicates

(4) Cytoplasmic and nuclear NFATc3 cooperatively contributes to vascular smooth muscle cell dysfunction and drives aortic aneurysm and dissectionActa Pharmaceutica Sinica BMay 21, 2025Xiu Liu, Li Zhao, Deshen Liu, Lingna Zhao, Yonghua Tuo, Qinbao Peng, Fangze Huang, Zhengkun Song, Chuanjie Niu, Xiaoxia He, et al.B1 (#13435; 1:1000), anti-phospho-eEF2 (Thr56) (#2331; 1:1000), anti-eEF2 (#2332; 1:1000), eEF2k (#3692; 1:1000) (Cell Signaling Technology), anti-LDL-R (sc-11824; 1:500; Santa Cruz Biotechnology, Dallas,

(5) Development of silver-based hybrid nanoparticles loaded with eEF2 K-siRNA and quercetin against triple-negative breast cancerDrug Delivery and Translational ResearchApril 23, 2025Orhan Burak Eksi, Ahsen Guler, Munevver Akdeniz, Pinar Atalay, Zuhal Hamurcu, Omer AydinTBS-T, the membranes were incubated with the following primary antibodies: eEF2 K (Cell Signaling, cat# 3692S), caspase- 3 (Proteintech, cat# 19,677–1-AP), cleaved caspase- 3 (Elabscience, cat# E-AB- 30004), AIF

(6) Neural stimulation suppresses mTORC1-mediated protein synthesis in skeletal muscleSci AdvApril 4, 2025Ana G. Dumitras, Giorgia Piccoli, Frederik Tellkamp, Lena Keufgens, Martina Baraldo, Sabrina Zorzato, Laura Cussonneau, Leonardo Nogara, Marcus Krüger, Bert Blaauw(Cell Signaling, 2332S), Phospho-eEF2k (Ser366) (Cell Signaling; ref. 3691), eEF2k (Cell Signaling; ref. 3692), phospho-AMPKα (Thr172) (Cell Signaling; ref. 2535), anti-AMPKα (Cell Signaling; ref: 2532S), anti-Phospho-ACC

(7) Eukaryotic Elongation Factor 2 Kinase EFK-1/eEF2K promotes starvation resistance by preventing oxidative damage in C. elegansNat CommunFebruary 18, 2025Junran Yan, Forum Bhanshali, Chiaki Shuzenji, Tsultrim T. Mendenhall, Shane K. B. Taylor, Glafira Ermakova, Xuanjin Cheng, Pamela Bai, Gahan Diwan, Donna Seraj, et al.antibodies were diluted in 5% skimmed milk as above, except for 1:1000 anti-rabbit eEF2K antibody (#3692, Cell Signaling Technologies), and α-tubulin which was used at 1:5000 concentration. Anti-rabbit (#7074,

(8) Subcellular functions of tau mediates repair response and synaptic homeostasis in injuryResearch SquareMarch 1, 2024David Grosshans, Riya Thomas, Die Zhang, Christopher Cronkite, Rintu Thomas, Sanjay Singh, Lawrence Bronk, Rodrigo Morales, Joseph Dumanphospho-tau-AT8 (#MN1020, ThermoFisher), anti-tau-1 (#MAB3420, Millipore Sigma), eEF2, eEF2K, β-tubulin (#2332, #3692S, #2146S, Cell Signaling), Nestin (#NB100–1604, Novus). Samples were washed with PBS containing 0.1%

(9) IL-6 translation is a therapeutic target of human cytokine release syndromeJournal of Experimental MedicineNovember 6, 2023Yuzhuo Yang, Yajing Zhang, Xiaoyan Xing, Gang Xu, Xin Lin, Yao Wang, Meixia Chen, Chunmeng Wang, Bin Zhang, Weidong Han, et al.BSA/Tris Buffered Saline with Tween 20: Phospho-eEF2k (Ser366; 1:1,000, 3691; CST), eEF2k (1:1,000, 3692; CST), Phospho-eEF2 (Thr56; 1:1,000, 2331; CST), eEF2 (1:1000,2332; CST), and β-actin (1:1,000, ac026;

(10) "Can endocan serve as a molecular ""hepatostat"" in liver regeneration?"Molecular medicine (Cambridge, Mass.)February 27, 2023Yazici SE, Gedik ME, Leblebici CB, Kosemehmetoglu K, Gunaydin G, Dogrul AB.endostatin (COL18A1 Antibody, Cat. # sc-32720, Santa Cruz Biotechnology, Dallas, Texas, USA), eEF2K (Cat. # 3692S, Cell Signaling Technology Inc., Danvers, Massachusetts, USA) and phospho-eEF2K (Cat. # 3691S, Cell Signaling Technology

(11) Functional high-throughput screen identifies microRNAs that promote butyrate-induced death in colorectal cancer cellsMolecular therapy. Nucleic acidsDecember 13, 2022Ali SR, Humphreys KJ, Simpson KJ, McKinnon RA, Meech R, Michael MZ.(92G2) Rabbit Monoclonal Antibody (mAb) #2978, COX2 Antibody #4842, Dvl3 Antibody #3218, eEF2k Antibody #3692, Met (D1C2) XP Rabbit mAb #8198, PI3 Kinase p55 (D2B3) Rabbit mAb #11889 (Cell Signaling Technology),

(12) Discovery of New Inhibitors of eEF2K from Traditional Chinese Medicine Based on In Silico Screening and In Vitro Experimental ValidationMoleculesJuly 30, 2022Qinghua Fu, Xiaomei Liu, Yan Li, Peng Wang, Tian Wu, Haihan Xiao, Yameng Zhao, Qichao Liao, Ziyi Songprimary antibody overnight at 4 °C. The following primary antibodies and dilutions were used: anti-eEF2K (3692S, 1:1000), anti-eEF2 (2332S, 1:1000), anti-phospho-eEF2 (Thr56) (2331S, 1:1000) and anti-α-tubulin antibody

(13) Elongation factor-2 kinase is a critical determinant of the fate and antitumor immunity of CD8 ⁺ T cellsScience AdvancesFebruary 4, 2022Jugal Kishore Das et al.in 2% bovine serum albumin and subsequently probed with primary antibodies for eEF-2K (catalog no. 3692, Cell Signaling Technology, Danvers, MA), phospho-Akt (S472, clone no. 104A282, BD Biosciences, San

(14) Homozygous knockout of eEF2K alleviates cognitive deficits in APP/PS1 Alzheimer's disease model mice independent of brain amyloid β pathologyFrontiers in aging neuroscienceJanuary 1, 2022Kasica NP, Zhou X, Jester HM, Holland CE, Ryazanov AG, Forshaw TE, Furdui CM, Ma T.BSA/TBST. Blots were probed with primary antibodies for: eEF2K (1:500, Cell Signaling Technology, catalog 3692), phospho-eEF2 (Thr56) (1:1,000, Cell Signaling Technology, catalog 2331); eEF2 (1:1,000, Cell Signaling Technology

(15) Inhibition of eEF2K synergizes with glutaminase inhibitors or 4EBP1 depletion to suppress growth of triple-negative breast cancer cellsScientific ReportsDecember 1, 2021YoungJun Ju, Yaacov Ben-David, Daniela Rotin, Eldad Zacksenhaustotal proteins were loaded. All primary antibodies were used at 1:500 dilution: rabbit anti-eEF2K (#3692, Cell Signaling Technology), rabbit anti-phospho-eEF2 (Thr56) (#2331, Cell Signaling Technology), rabbit

(16) Integrative proteomics reveals the role of E3 ubiquitin ligase SYVN1 in hepatocellular carcinoma metastasisCancer CommunicationsOctober 1, 2021Feiyang Ji, Menghao Zhou, Zeyu Sun, Zhengyi Jiang, Huihui Zhu, Zhongyang Xie, Xiaoxi Ouyang, Lingjian Zhang, Lanjuan Limouse monoclonal anti‐HSP90 (Cat#sc‐13119, Santa, Dallas, TX, USA), rabbit polyclonal anti‐EEF2K (Cat#3692S, Cell Signaling Technology), mouse monoclonal anti‐Flag (Cat#sc‐51590, Santa), or mouse monoclonal anti‐CD31

(17) Effects of a High-Grain Diet With a Buffering Agent on Milk Protein Synthesis in Lactating GoatsFrontiers in Veterinary ScienceJuly 6, 2021Meilin He, Xintian Nie, Huanhuan Wang, Shuping Yan, Yuanshu Zhangrb-anti-phosphorylated-eIF4E (rb-anti-p-eIF4E, #9741S), rb-anti-eukaryotic elongation factor-2 kinase (rb-anti-eEF2K, #3692S), rb-anti-phosphorylated-eukaryotic elongation factor-2 kinase (rb-anti-p-eEF2K, #3691S), and rb-anti-eukaryotic

(18) Autophagy inhibition and microRNA‑199a‑5p upregulation in paclitaxel‑resistant A549/T lung cancer cellsOncology reportsJuly 1, 2021Zeng T, Xu M, Zhang W, Gu X, Zhao F, Liu X, Zhang X.follows: LC3B (1:800; cat. no. 2775S), eukaryotic elongation factor 2 kinase (eEF2K; 1:1,000; cat. no. 3692S), PI3K (1:1,000; cat. no. 4263S), Akt (1:1,000; cat. no. 4691S), phosphor (p)-Akt (S473, D9E; 1:1,000;

(19) Rapamycin Extends Life Span in Apc^Min/+ Colon Cancer FAP ModelClinical colorectal cancerMarch 1, 2021Parihar M, Dodds SG, Hubbard G, Javors MA, Strong R, Hasty P, Sharp ZD.(1:2000, Santa Cruz Biotechnology, sc-365062) and rabbit anti eEF2k (1:800, Cell Signaling Technology; 3692). Hematocrits We collected blood at the time of sacrifice by cheek bleeding. Cells from 50–75 μL of

(20) The eEF2 kinase-induced STAT3 inactivation inhibits lung cancer cell proliferation by phosphorylation of PKM2Cell communication and signaling : CCSFebruary 13, 2020Xiao M, Xie J, Wu Y, Wang G, Qi X, Liu Z, Wang Y, Wang X, Hoque A, Oakhill J, et al.AKT (#4685), p-AKT (#9614), Src (#12109), p-Src (#12432), STAT3 (#12640), p-STAT3 (#9145), eEF2K (#3692), eEF2 (#2332), p-eEF2 (#2331), c-Myc(#2729), and PKM2 (#4053) were obtained from Cell Signaling Technology.

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eEF2k Antibody

eEF2k Antibody from Cell Signaling Technology

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Cell Signaling Technology

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eEF2k Antibody from Cell Signaling Technology

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