beta-Tubulin (9F3) Rabbit mAb (HRP Conjugate) 5346S from Cell Signaling Technology

Product Specs
Itembeta-Tubulin (9F3) Rabbit mAb (HRP Conjugate)
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number5346S
Quantity100 µl
ApplicationsW
ReactivityH, M, R, Mk, Z, B
Targettubulin beta-5
HostRabbit
NCBI Gene AliasesCDCBM6, CSCSC1, M40, OK/SW-cl.56, TUBB1, TUBB5
Conjugate/TagHRP
ClonalityMonoclonal
NCBI Full Gene Nametubulin beta class I
Research AreaCytoskeletal Signaling
IsotypeIgG
Clone9F3
Molecular Weight55

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Nanoscale organization of the endogenous ASC speckiScienceDecember 15, 2023Ivo M. Glück, Grusha Primal Mathias, Sebastian Strauss, Virgile Rat, Irene Gialdini, Thomas Sebastian Ebert, Che Stafford, Ganesh Agam, Suliana Manley, Veit Hornung, et al.HASC-71) (Biolegend) at a final concentration of 33.5 nM (5 μg/mL) and primary rabbit β-tubulin antibody (5346, Cell Signaling Technologies, Danvers, Massachusetts, USA). As secondary probe, an anti-mouse nanobody

(2) The protective role of CD73 in periodontitis: preventing hyper-inflammatory fibroblasts and driving osteoclast energy metabolismFront Oral HealthDecember 13, 2023Erivan S. Ramos-Junior, Shantiece Dawson, Weston Ryan, Braden Clinebell, Rogelio Serrano-Lopez, Marsha Russell, Rylee Brumbaugh, Roger Zhong, Jussara Gonçalves Fernandes, Luciana M. Shaddox, et al.Antibodies from Cell Signaling: Catalase (1:1,000, #14097); HRP Conjugate β-Tubulin (9F3) (1:1,000, #5346); HRP conjugate H3 (1:1,000 # 12648), HRP conjugate β-Actin (1:1,000 # 12620); HRP Conjugate GAPDH (1:1,000,

(3) Clinically Defined Mutations in MEN1 Alter Its Tumor-suppressive Function Through Increased Menin TurnoverCancer Research CommunicationsJuly 24, 2023Suzann Duan, Sulaiman Sheriff, Uloma B. Elvis-Offiah, Brandon L. Witten, Travis W. Sawyer, Sinju Sundaresan, Tomasz Cierpicki, Jolanta Grembecka, Juanita L. MerchantCell Signaling Technology), Rabbit anti-β-Tubulin HRP-conjugated antibody (1:2,000 dilution, catalog no. 5346, Clone 9F3, RRID: AB_1950376, Cell Signaling Technology), Rabbit anti-Histone H3 HRP-conjugated antibody

(4) Endothelial-derived FABP4 constitutes the majority of basal circulating hormone and regulates lipolysis-driven insulin secretionJCI InsightJuly 24, 2023Karen E. Inouye, Kacey J. Prentice, Alexandra Lee, Zeqiu B. Wang, Carla Dominguez-Gonzalez, Mu Xian Chen, Jillian K. Riveros, M. Furkan Burak, Grace Y. Lee, Gökhan S. Hotamışlıgilwere incubated with HRP-tagged anti–β-tubulin antibody (Abcam, ab21058; Cell Signaling Technology, 5346) to measure β-tubulin as a loading control. HRP signal was detected with chemiluminescent substrate

(5) Metabolic reprogramming through mitochondrial biogenesis drives adenosine anti-inflammatory effects: new mechanism controlling gingival fibroblast hyper-inflammatory stateFrontiers in ImmunologyJune 7, 2023Nathalie Paladines, Shantiece Dawson, Weston Ryan, Rogelio Serrano-Lopez, Regina Messer, Yuqing Huo, Christopher W. Cutler, Erivan S. Ramos-Junior, Ana Carolina Morandini(Thr172) (40H9) (1:1000, #2535); AMPKα (D5A2) (1:1000, #5831); HRP Conjugate β-Tubulin (9F3) (1:1000, #5346); SIRT1 (D1D7) (1:1000, #9475); Hexokinase II (C64G5) (1:1000, #2867); PKM2 (D78A4) XP (1:1000, #4053);

(6) Schlafen4+-MDSC in Helicobacter-induced gastric metaplasia reveals role for GTPasesFrontiers in ImmunologyJune 2, 2023Lin Ding, Sulaiman Sheriff, Ricky A. Sontz, Juanita L. Merchant#ab283655), CHOP (Abcam, #ab11419), cleaved Caspase-3 (Cell Signaling, #9661), β-Tubulin (Cell signaling, #5346S), GAPDH (MA5-15738, ThermoFisher). Nucleotide extraction and ATP/GTP assay The extraction of nucleotides

(7) The long-range interaction between two GNAS imprinting control regions delineates pseudohypoparathyroidism type 1B pathogenesisJournal of Clinical InvestigationApril 17, 2023Yorihiro Iwasaki, Cagri Aksu, Monica Reyes, Birol Ay, Qing He, Murat Bastepeanti-OCT4 and anti-SOX2 antibodies described above. Anti–β-tubulin antibody (Cell Signaling Technology, 5346) was used as a loading control. Statistics. Statistical analyses were performed using GraphPad Prism

(8) A flagellin-conjugate protein induces dual NLRC4- and NLRP3-inflammasome activation which modulates inflammatory cytokine secretion from macrophagesFrontiers in ImmunologyMarch 21, 2023Yen-Ju Lin, Annette Jamin, Sonja Wolfheimer, Anna Fiedler, Ann-Christine Junker, Alexandra Goretzki, Stephan Scheurer, Stefan SchülkeCell Signaling Technologies), anti-Caspase-1 antibody (#3866, Cell Signaling Technologies), anti-β-Tubulin antibody (#5346, Cell Signaling Technologies), anti-ASC antibody (#AG-25B-0006-C100, Adipogen, Fuellinsdorf, Switzerland),

(9) Multigenerational mistimed feeding drives circadian reprogramming with an impaired unfolded protein responseFront Endocrinol (Lausanne)March 6, 2023Kai Huang, Tao Zhang, Wenjun Zhang, Yue Gu, Pan Yu, Lanqing Sun, Zhiwei Liu, Tao Wang, Ying Xufor the experiments. Antibodies against β-actin (1:5,000, A5441; Sigma-Aldrich), β-Tubulin (1:5,000, #5346; Cell Signaling), CHOP (1:1,000, #2895; Cell Signaling), Procaspase-3 (1:1,000, #9662; Cell Signaling),

(10) Deregulation of mTORC1-TFEB axis in human iPSC model of GBA1-associated Parkinson's diseaseFrontiers in neuroscienceJanuary 1, 2023Mubariz F, Saadin A, Lingenfelter N, Sarkar C, Banerjee A, Lipinski MM, Awad O.Anti-β-Actin-HRP conjugated antibody (Sigma-Aldrich, A3854), anti β-tubulin antibody (Cell Signaling, 5346S), or anti-GAPDH (Sigma-Aldrich, G8795) were used to control for loading. Membranes were developed using

(11) Antidiabetic drug metformin suppresses tumorigenesis through inhibition of mevalonate pathway enzyme HMGCS1The Journal of biological chemistryDecember 1, 2022Chen Y, Li M, Yang Y, Lu Y, Li X.The antibodies against GAPDH (catalog no.: 3683; Cell Signaling Technology), tubulin (catalog no.: 5346; Cell Signaling Technology), and histone H3 (catalog no.: AH433; Beyotime) served as internal references

(12) Apoptosis in platelets is independent of the actin cytoskeletonPLoS OneNovember 15, 2022Enoli De Silva, Manoj Paul, Hugh Kimanti-gelsolin antibody (catalogue #: 12953) and the HRP-conjugated anti-beta-tubulin antibody (catalogue #: 5346) were purchased from Cell Signaling Technologies (Danvers, MA). The HRP-conjugated anti-beta-actin antibody

(13) Role of Glycolysis and Fatty Acid Synthesis in the Activation and T Cell-Modulating Potential of Dendritic Cells Stimulated with a TLR5-Ligand Allergen Fusion ProteinInternational Journal of Molecular SciencesOctober 21, 2022Alexandra Goretzki, Yen-Ju Lin, Jennifer Zimmermann, Hannah Rainer, Ann-Christine Junker, Sonja Wolfheimer, Stefan Vieths, Stephan Scheurer, Stefan SchÃ¼lkeantibody (CST, Cat #7074, 1:5000). β-tubulin was detected using: HRP-conjugated anti-β-tubulin (CST, Cat #5346, 1:5000). Images were captured with an iBright™ CL1500 system (Thermo Fischer Scientific), and the intensities

(14) Saturation of the mitochondrial NADH shuttles drives aerobic glycolysis in proliferating cellsMolecular cellSeptember 1, 2022Wang Y, Stancliffe E, Fowle-Grider R, Wang R, Wang C, Schwaiger-Haber M, Shriver LP, Patti GJ.respectively. Immunoblotting for β-tubulin by rabbit anti-β-tubulin antibody (HRP conjugated) (Cat# 5346, RRID:AB_1950376) (Cell Signaling) was used as a loading control. Signal was detected by using WesternSure

(15) Preliminary mechanistic insights of a brain-penetrant microtubule imaging PET ligand in a tau-knockout mouse modelEJNMMI researchJuly 26, 2022Damuka N, Orr ME, Bansode AH, Krizan I, Miller M, Lee J, Macauley SL, Whitlow CT, Mintz A, Craft S, et al.α-Tubulin (Cat. # 3873), Acetyl α-Tubulin (Cat. # 5335T), β-Tubulin (9F3) Rabbit mAb HRP Conjugate (cat # 5346) and α-Tubulin (DM1A) Mouse mAb HRP Conjugate (cat #12351), and HRP-conjugated secondary antibody (Cat.

(16) Epigallocatechin gallate (EGCG) attenuates severe acute respiratory coronavirus disease 2 (SARS-CoV-2) infection by blocking the interaction of SARS-CoV-2 spike protein receptor-binding domain to human angiotensin-converting enzyme 2PLOS ONEJuly 13, 2022Tomokazu Ohishi, Takayuki Hishiki, Mirza S. Baig, Sajjan Rajpoot, Uzma Saqib, Tomohiko Takasaki, Yukihiko Haraantibodies: anti-ACE2 antibody (ab273433, 1:1000; Abcam, Cambridge, UK) and anti-β-tubulin antibody (#5346, 1:1000; Cell Signaling Technology, Danvers, MA, USA). Proliferation assays in vitro We measured cell

(17) Decoupling PER phosphorylation, stability and rhythmic expression from circadian clock function by abolishing PER-CK1 interactionNature CommunicationsJuly 9, 2022Yang An, Baoshi Yuan, Pancheng Xie, Yue Gu, Zhiwei Liu, Tao Wang, Zhihao Li, Ying Xu, Yi Liu1:1000), HA (Sigma-Aldrich, A2095, 1:1000), Actin (Sigma-Aldrich, A5441, 1:2000) and Tubulin (CST, 5346 s, 1:3000). BMAl1 and CRY1 used in WB (BMAL1 1:1000, CRY1 1:1000), CoIP and ChIP were previously described84.

(18) Molecular regulation of lung maturation in near-term fetal sheep by maternal daily vitamin C treatment in late gestationPediatric ResearchMarch 1, 2022Erin V. McGillick et al.Cell Signaling Technology; 42 kDa band), β-tubulin (1:10,000 in 5% BSA in TBS-T, β-tubulin (9F3) HRP conjugate, #5346, Cell Signaling Technology; 55 kDa band), or COX1V (3E11) (1:10,000 in 5% BSA in TBS-T, COX1V (3E11)

(19) Cholesterol biosynthesis defines oligodendrocyte precursor heterogeneity between brain and spinal cordCell reportsMarch 1, 2022Khandker L, Jeffries MA, Chang YJ, Mather ML, Evangelou AV, Bourne JN, Tafreshi AK, Ornelas IM, Bozdagi-Gunal O, Macklin WB, et al.(Covance, SMI-99, 1:500), PLP (Clone AA3, gift from the Macklin Lab, 1:40000), β-tubulin (Cell Signaling, 5346S, 1:1000), and β-amyloid (Invitrogen, 51–2700, 1:1000), SMI-32 (Biolegend, 801701, 1:500). Statistical

(20) Role of mTORC1 activity during early retinal development and lamination in human-induced pluripotent stem cell-derived retinal organoidsCell death discoveryFebruary 8, 2022Lee SH, Han JW, Yang JY, Jun HO, Bang JH, Shin H, Choi JH, Lee J, Madrakhimov SB, Chung KH, et al.Biologicals, Shirley, MA), p-S2448 (1:250; 44-1125G, Invitrogen, Waltham, MA), p-S6 ribosomal protein (1:500; 5346, Cell Signaling Technology, Danvers, MA), AtoH7 (1:500; PA5-35859, Thermo Fisher Scientific). Species-specific

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10/2/2020ß-Tubulin (9F3) Rabbit mAb (HRP Conjugate) for Western Blot Sachin SamuchiwalI used this antibody as a loading control for my samples stimulated with different compounds of interest.

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beta-Tubulin (9F3) Rabbit mAb (HRP Conjugate) from Cell Signaling Technology

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Cell Signaling Technology

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beta-Tubulin (9F3) Rabbit mAb (HRP Conjugate) from Cell Signaling Technology

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