AMPKalpha (23A3) Rabbit mAb 2603S from Cell Signaling Technology

Product Specs
ItemAMPKalpha (23A3) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number2603S
ClonalityMonoclonal
ApplicationsWestern Blot (WB)
TargetAMPK-alpha1;AMPK-alpha2
NCBI Full Gene Nameprotein kinase AMP-activated catalytic subunit alpha 2, protein kinase AMP-activated catalytic subunit alpha 1
NCBI Gene AliasesAMPK, AMPK alpha 1, PRKAA, AMPK2, AMPKa1, AMPKa2
Research AreaMetabolism
IsotypeIgG
Clone23A3
Molecular Weight62
ReactivityHu, Ms, Rt, Mk
HostRabbit
Quantity100 µl

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Distal Venous Arterialization Modulates Autophagy-Related Markers in Skeletal Muscle in a Rat Hindlimb Ischemia ModelVasc Health Risk ManagMarch 24, 2026Teguh Marfen Djajakusumah, Bethy Suryawathy Hernowo, Pei Ho, Herry Herman, Kiki Lukman, Ronny Lesmanaantibodies (Cell Signaling Technology) were used at 1:1000 dilution: anti-HIF-1α (#3716), anti-AMPKα (#2603), anti-LC3 (#12741), anti-p62/SQSTM1 (#8025), and anti-β-actin (#4967). After HRP-conjugated secondary

(2) Benefits of sacubitril/valsartan alone and in combination with dapagliflozin in a preclinical female model of cardiometabolic and age-related cardiac dysfunctionCardiovascular DiabetologyMarch 21, 2026Elena Mele, Maria Antonietta Riemma, Maria Donniacuo, Marialucia Telesca, Grazia Sorrentino, Domenico D’Amario, Filippo Scialò, Eleonora Cianflone, Donato Cappetta, Daniele Torella, et al.(40H9),Rabbit,1:1000,Cell Signaling Technology 2535 S AMPKalpha (23A3),Rabbit,1:1000,Cell Signaling Technology 2603 S NAMPT,Mouse,1:1000,ThermoFisher ScientificMA5-43720 Klotho,Rabbit,1:1000,ThermoFisher ScientificPA5-21078 Anti-

(3) Neuronal PPP2R5C in plasma is a potential biomarker for early diagnosis of Alzheimer’s diseaseCell Reports MedicineMarch 17, 2026Shilin Luo, Hui Liu, Tingting Xiao, Yunni Li, Xixi Liu, Xuewen Xiao, Xinxin Liao, Yingzi Liu, Yafang Zhou, Jun-Ling Wang, et al.#2971",RRID:AB_330970 p-AMPKα,"Cell Signaling Technology, #2535",RRID:AB_331250 AMPKα,"Cell Signaling Technology, #2603",RRID:AB_490795 p-P70S6K,"Proteintech, #28735-1-AP",RRID:AB_2918197 P70S6K,"Proteintech, #14485-1-AP",RRID:AB_2269787 p-ACC,"Cell Signaling Technology

(4) mTOR-driven autophagy suppression defines metabolic vulnerability in CDK4/6 inhibitor-resistant HR+/HER2− breast cancerCell Death & DiseaseFebruary 19, 2026Luise von Wichert, Alina Stroh, Marie Witt, Michael Wanzel, Marco Mernberger, Sebastian Griewing, Thomas Wündisch, Berit M. Pfitzner, Julia Teply-Szymanski, Anne-Sophie Litmeyer, et al.(H9) (1:500, sc-8418, Santa Cruz Biotechnology), ULK1 (1:1000, #8054, Cell Signaling), AMPKα (1:1000, #2603, Cell Signaling), Acetyl-CoA Carboxylase (1:1000, #3676, Cell Signaling), β-Actin (AC-15) (1:10.000,

(5) Protein kinase C epsilon deletion in AgRP neurons modulates hypothalamic glucose sensing and improves glucose tolerance in miceMolecular MetabolismFebruary 1, 2026Amanda E. Brandon, Chenxu Yan, Xuan Zhang, Chi Kin Ip, Zhongmin Gao, Nicola J. Lee, Oana C. Marian, Alex Perez, Anthony S. Don, Herbert Herzog, et al.phospho-Ser159/163 MARCKS (Cell Signalling Technology #11992), total AMPKα (Cell Signalling Technology #2603) and phospho-Thr172 AMPKα Cell Signalling Technology # 2535), 14-3-3 protein (Santa Cruz (H-8), sc-1657)

(6) Enhanced Retinal Ganglion Cell Survival via Autophagy Activation in a Novel Retinal Ischemia/Reperfusion Rat ModelInternational Journal of Molecular SciencesJanuary 20, 2026Si Hyung Lee, Jung Woo Han, Su-ah Yoon, Hun Soo Chang, Tae Kwann Park"Cell Signaling, 8054",WB 1/1000 p-ULK1 (Ser757),"Cell Signaling, 14202S",WB 1/1000 AMPK,"Cell Signaling, 2603",WB 1/1000 p-AMPK (Thr172),"Cell Signaling, 2535",WB 1/1000 p62,"Sigma, P0067 (Burlington, MA, USA)",WB

(7) Inactive ryanodine receptors sustain lysosomal availability for autophagy by promoting ER-lysosomal contact site formationNature CommunicationsJanuary 15, 2026Tim Vervliet, Jens Loncke, Marko Sever, Karan Ahuja, Chris Van den Haute, Tomas Luyten, Grace E. Stutzmann, Catherine Verfaillie, Tihomir Tomašič, Geert Bultynck(#V-9131, Merck, 1:5000), anti-phospho-AMPK (2535S, Cell signaling technology, 1:1000), anti-AMPK (2603S, Cell signaling technology, 1:1000), anti-phospho-p70 S6 kinase (9234S, Cell signaling technology, 1:1000),

(8) An ATF3 Inducer Ameliorates Metabolic Dysfunction-Associated Steatotic Liver Disease Through the AMPK and PKA PathwaysInt J Mol SciDecember 9, 2025Ching-Feng Cheng, Ruey-Bing Yang, Wen-Ting Chen, Jia-Fang Chung, Hui-Chen KuPVDF membranes (Merck Millipore, Burlington, MA, USA), and probed with antibodies, including AMPKα (#2603, Cell Signaling Technology), Phospho-AMPKα (Thr172) (#2535, Cell Signaling Technology, Danvers, MA,

(9) Hepatic activating transcription factor 3 protects against systemic inflammation by attenuating lipotoxicityMetabolism OpenDecember 1, 2025Chencheng Hu, Qian Yang, Runzhi Yu, Shuwei Hu, Bing Meng, Yanyong Xupurchased from Proteintech Group Inc. Antibodies against ACC (catalog no. 3662S), AMPKα (catalog no. 2603), or pAMPKα (catalog no. 2535) were purchased from Cell Signaling Technology. 2.9 Oil red O staining Liver

(10) Obestatin treatment links mitochondrial homeostasis and skeletal muscle repair in Duchenne muscle dystrophyMolecular BiomedicineNovember 25, 2025Andrea C. Lodeiro, Silvia Costas-Abalde, Tania Cid-Díaz, Lucía Debasa-Corral, Saúl Leal-López, Kamel Mamchaoui, Vincent Mouly, Xesús Casabiell, Rosalía Gallego, José Luis Relova, et al.antibody,Supplier,Reference,Use,Dilution Actin,Abcam,ab1801,WB,1:1000 "α-Bungarotoxin (α-BTX), Alexa Fluor™ 488 conjugate",Invitrogen,B13422,IF,1:500 AMPKα,Cell Signaling,2603,WB,1:1000 PPP3CA,Invitrogen,"PA5-82,471",WB,"0,2 µg/ml" DRP1,Cell Signaling,8570,WB,1:1000 β-dystroglycan,Santa Cruz

(11) The designer cytokine IC7Fc attenuates atherosclerosis development by targeting hyperlipidemia in miceSci AdvOctober 24, 2025Wietse In Het Panhuis, Ellen Thiemann, Daisy M. A. H. van Dijk, Bibian Been, Kelsey E. Jarrett, Amber Meurs, Sander Kooijman, Milaine V. Hovingh, Melanie Modder, Bram W. van Os, et al.antibodies from Cell Signaling (MA, USA) were used: phospho-AMPKα (Thr172) (#2535, 1:50), AMPKα (23A3) (#2603, 1:50), phospho-acetyl-CoA carboxylase (Ser79) (#3661, 1:20), acetyl-CoA carboxylase (#3662, 1:20),

(12) AKT-mediated phosphorylation of TSC2 controls stimulus- and tissue-specific mTORC1 signaling and organ growthDevelopmental CellOctober 6, 2025Yann Cormerais, Samuel C. Lapp, Krystle C. Kalafut, Madi Y. Cissé, Jong Shin, Benjamin Stefadu, Jean Personnaz, Sandra Schrötter, Jessica Freed, Angelica D’Amore, et al.PRAS40 (CST, 2610), P-PRAS40 T246 (CST, 2997), mTOR (CST, 2983), P-mTOR S2448 (CST, 2971), AMPKα (CST, 2603), P-AMPKα T172 (CST, 2535), P-TFEB S122 (CST, 87932), TFEB (Bethyl Laboratories, A303–673A) and Puromycin

(13) Dual Impact of Iron Deficiency and Antibiotics on Host Metabolism: A Tissue-Level AnalysisMetabolitesAugust 14, 2025Shannon Shoff, Sydney Thomas, Peng Ji, Mariana Parenti, Carolyn M. Slupsky(Cell Signaling Technology, Boston, MA, USA): AKT (#9272), p-AKT(Ser473) (#4060), p-AKT(Thr308) (#13038), AMPKα (#2603), p-AMPKα(Thr172) (#2535), mTOR (#2972), p-mTOR(Ser2448) (#5536), p70-S6K(Thr389) (#9202), and p-p70-S6K(Thr389)

(14) Pemafibrate Ameliorates Steatotic Liver Disease Regardless of Endothelial Dysfunction in MiceAntioxidants (Basel)July 20, 2025Tomoyo Hara, Hiroki Yamagami, Ryoko Uemoto, Akiko Sekine, Yousuke Kaneko, Kohsuke Miyataka, Taiki Hori, Mayuko Ichimura-Shimizu, Masafumi Funamoto, Takeshi Harada, et al.phosphorylated Akt (Ser473) (CST, Inc., cat. no. 9271, Danvers, MA, USA), AMPKα (CST, Inc., cat. no. 2603, Danvers, MA, USA), phosphorylated AMPKα (Thr172) (CST, Inc., cat. no. 2535, Danvers, MA, USA), glyceraldehyde-3-phosphate

(15) RDM1 plays an oncogenic role in clear cell renal cell carcinoma potentially by modulating MCM2Scientific reportsJuly 2, 2025Li X, Liu H, Wei Y, Wang A, Lv C, Zeng Y.Proteintech, 1:500), anti-MCM2 (3619, CST, 1:1000), anti-FLAG (14793 S, CST, 1:1000), anti-PRKAA1 (2603, CST, 1:1000), anti-SKP2 (ab183039, Abcam, 1:200), anti-EPS15 (ab174291, Abcam, 1:1000), anti-RALB (ab156799,

(16) Protein O-GlcNAcylation and hexokinase mitochondrial dissociation drive heart failure with preserved ejection fractionCell MetabolismJuly 1, 2025Yuki Tatekoshi, Amir Mahmoodzadeh, Jason S. Shapiro, Mingyang Liu, George M. Bianco, Ayumi Tatekoshi, Spencer Duncan Camp, Adam De Jesus, Navid Koleini, Santiago De La Torre, et al.ATP-synthase-C (ab181243, Abcam); Phospho-AMPKα (Thr172) (4188, Cell Signaling Technology); AMPKα (2603, Cell Signaling Technology); GFPT1 (14132–1-Ig, Proteintech); RPN1 (12894–1-AP, Proteintech); RPN2 (10576–1-AP,

(17) ALDH7A1 protects against ferroptosis by generating membrane NADH and regulating FSP1CellMay 15, 2025Jia-Shu Yang, Andrew J. Morris, Koki Kamizaki, Jianzhong Chen, Jillian Stark, William M. Oldham, Toshitaka Nakamura, Eikan Mishima, Joseph Loscalzo, Yasuhiro Minami, et al.rabbit polyclonal (WB 1:5000), Santa Cruz], ACSL4 (mouse monoclonal, Santa Cruz, WB 1:100), AMPKα1 (2603S, Cell signaling, WB 1:500), phospho-substrates of AMPK (5759S, Cell Signaling, WB 1:500), 6xHis epitope

(18) Ergosterol Peroxide Disrupts Triple-Negative Breast Cancer Mitochondrial Function and Inhibits Tumor Growth and MetastasisInt J Mol SciMay 10, 2025Aliyah L. Bocachica-Adorno, Adriana Y. Aponte-Ramos, Paola S. Rivera-Fuentes, Natalia P. Espinosa-Ponce, Luz V. Arroyo-Cruz, Taotao Ling, Naydi Pérez-Ríos, Sona Rivas-Tumanyan, Israel A. Almodóvar-Rivera, Carlos Barreto-Gamarra, et al.at the dilutions recommended by the manufacturer. The following antibodies were used: AMPKα (23A3) (#2603P), Phospho-AMPKα (Thr172) (40H9) (#2535P), SOX2 (D6D9) (#3579S), NANOG (D73G4) (#4903S), OCT4 (C30A3)

(19) Role of SIRT3 in the regulation of Gadd45α expression and DNA repair in β-cellsJournal of Biological ChemistryMay 1, 2025Aaron Naatz, Kelsey S. Bohl, Rachel A. Jones Lipinski, Joshua A. Nord, Alyssa L. Gehant, Polly A. Hansen, Brian C. Smith, John A. Corbettantibodies were purchased from Cell Signaling Technology: rabbit anti-FoxO1 (9454, 2880), rabbit anti-AMPK (2603), mouse anti-SIRT1 (8469), rabbit anti-SIRT2 (12,672), rabbit anti-SIRT3 (5490), rabbit anti-SIRT6 (12,486),

(20) Ibuprofen, Flurbiprofen or Naproxen Sodium Minimally Influences Musculoskeletal Adaptations to Treadmill Exercise in RatsJ Cachexia Sarcopenia MuscleApril 10, 2025Brandon M. Roberts, Alyssa V. Geddis, Alexandra Ciuciu, Marinaliz Reynoso, Nikhil Mehta, Alyssa N. Varanoske, Alyssa M. Kelley, Maximus C. Leiss, Alexander L. Kolb, Julie M. Hughes, et al.the following antibodies: p‐AKT S473 (#9271), Total AKT (#9272), p‐AMPKα T172 (#2535), Total AMPK α (#2603), p‐S6 S235/236 (#4858), p‐S6 S240/244 (#2215), Total S6 (#2217), p‐ERK 42/44 (#9101), Total ERK (#9102),

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AMPKalpha (23A3) Rabbit mAb

AMPKalpha (23A3) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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AMPKalpha (23A3) Rabbit mAb from Cell Signaling Technology

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