Clathrin Heavy Chain (P1663) Antibody 2410S from Cell Signaling Technology

Product Specs
ItemClathrin Heavy Chain (P1663) Antibody
CompanyCell Signaling Technology
Price $306.00 Inquire
Catalog Number2410S
Quantity100 µl
ApplicationsW, IF-IC
ReactivityH, M, R, Mk, B
TargetCLTC
HostRabbit
NCBI Gene AliasesCHC, CHC17, CLH-17, CLTCL2, Hc, MRD56
NCBI Full Gene Nameclathrin heavy chain
ClonalityPolyclonal
Research AreaProtein Folding and Trafficking
CloneP1663
Molecular Weight190

Questions and Answers

Can this antibody be used to study the role of clathrin in viral infections?

Yes, the Clathrin Heavy Chain antibody has been utilized in studies investigating the entry mechanisms of viruses such as the rabies virus, where clathrin-mediated endocytosis was shown to be essential for viral internalization in N2a cells ().

Is this antibody effective for detecting clathrin in different species?

The Clathrin Heavy Chain antibody is reactive with multiple species, including humans, mice, rats, non-human primates, and bovines, making it suitable for comparative studies across these organisms.

How has this antibody been applied in research related to neurological diseases?

This antibody has been used in studies examining the pathophysiological processes in severe West Nile virus infection, where alterations in clathrin-mediated endocytosis were linked to changes in cytoskeletal maintenance and inflammatory responses in mouse brain tissue ().

What experimental techniques can this antibody be used with?

The Clathrin Heavy Chain antibody is suitable for applications such as Western blotting, immunocytochemistry, and immunofluorescence, as demonstrated in various studies that utilized these techniques to analyze clathrin expression and localization (, ).

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Website: Technical Support

(1) Porcine reproductive and respiratory syndrome virus exploits ESCRT-II subunit EAP20 for entry and replicationJournal of VirologyApril 21, 2026Longxiang Zhang, Yan Jiang, Rui Li, Mengjie Wang, Xinrong Wang, Junhai Zhu, Nan Yan, Songlin Qiao, Rui Li, Yue Wangexperiments unless otherwise stated. Antibodies Rabbit anti-clathrin polyclonal antibodies (pAbs, #2410S), rabbit anti-Rab5 monoclonal antibody (MAb, #3547S), rabbit anti-Rab7 MAb (#9367S), and rabbit anti-Rab11

(2) Regulation of AP1 adaptor assembly by the bi-handed chaperone MEA1Nature CommunicationsJanuary 20, 2026Chun Wan, Jingyi Wu, Yan Ouyang, Harrison Puscher, Yuan Tian, Suzhao Li, Qian Yin, Jingshi ShenRRID:AB_10693472), polyclonal anti-clathrin heavy chain (CHC) antibodies (Cell Signaling Technology, #2410, RRID: AB_2083156), polyclonal anti-γ antibodies (Bethyl Laboratories, #A304-771A, RRID: AB_2620966),

(3) Hypothalamic tanycytes internalize ghrelin from the cerebrospinal fluid: Molecular mechanisms and functional implicationsMolecular MetabolismDecember 1, 2024Ivana M. Gomez, Maia Uriarte, Gimena Fernandez, Franco Barrile, Daniel Castrogiovanni, Sonia Cantel, Jean-Alain Fehrentz, Pablo N. De Francesco, Mario Perellocells pre-incubated with Fr-ghrelin were incubated either with anti-clathrin (Cell Signaling, cat. 2410, 1:1000), anti-rab5 (Cell Signaling, cat. 3547, 1:1000) or anti-transferrin receptor (TfR, Invitrogen,

(4) The AP-1 adaptor complex is essential for intracellular trafficking of the ORF2 capsid protein and assembly of Hepatitis E virusCellular and Molecular Life SciencesDecember 1, 2024Martin Ferrié, Virginie Alexandre, Claire Montpellier, Peggy Bouquet, Thibault Tubiana, Léa Mézière, Maliki Ankavay, Cyrine Bentaleb, Jean Dubuisson, Stéphane Bressanelli, et al.(2) AP-1G2,AP-1γ2,Rabbit,pAb,Sigma,HPA004106,AB_1078172,1/1000,n/a AP-2B1,AP-2B1,Rabbit,pAb,Proteintech,15690-1-AP,AB_2056351,n/a,1/200 (2) Clathrin,Clathrin heavy chain,Rabbit,pAb,Cell signaling,#2410,AB_2083156,n/a,1/100 (2) M6PR,Cation-independant Mannose-6-phosphate receptor,Rabbit,pAb,B. Hoflack,[60],n/a,n/a,1/500

(5) An AAGAB-to-CCDC32 handover mechanism controls the assembly of the AP2 adaptor complexProceedings of the National Academy of SciencesAugust 20, 2024Chun Wan, Harrison Puscher, Yan Ouyang, Jingyi Wu, Yuan Tian, Suzhao Li, Qian Yin, Jingshi ShenmScarlet-anti-ALFA nanobodies. Endogenous CHC was labeled using anti-CHC antibodies (Cell Signaling Technology, #2410, RRID: AB_2083156) and Alexa Fluor 647–conjugated secondary antibodies (Thermo Fisher Scientific, #A32733,

(6) Parkinson’s disease-linked parkin mutation disrupts recycling of synaptic vesicles in human dopaminergic neuronsNeuronDecember 1, 2023Pingping Song, Wesley Peng, Veronique Sauve, Rayan Fakih, Zhong Xie, Daniel Ysselstein, Talia Krainc, Yvette C. Wong, Niccolò E. Mencacci, Jeffrey N. Savas, et al.anti-a-synuclein (c-20),Santa Cruz,Cat#sc-7011-R; RRID: AB_2192953 Rabbit anti-Clathrin,Cell Signaling,Cat#2410; RRID: AB_2083156 Bacterial and virus strains,Bacterial and virus strains,Bacterial and virus strains Lenti-parkin

(7) Chloroquine and cytosolic galectins affect endosomal escape of antisense oligonucleotides after Stabilin-mediated endocytosisMolecular Therapy - Nucleic AcidsSeptember 12, 2023Ekta Pandey, Edward N. Harris#4408S) was used for detecting these primary antibodies. Primary antibodies for clathrin (catalog #2410S), caveolin 1 (catalog #3238S), early endosomal antigen (EEA1) (catalog #2411S), Rab7 (catalog #9367S),

(8) Evolutionary origins and interactomes of human, young microproteins and small peptides translated from short open reading framesMolecular CellMarch 16, 2023Clara-L. Sandmann, Jana F. Schulz, Jorge Ruiz-Orera, Marieluise Kirchner, Matthias Ziehm, Eleonora Adami, Maike Marczenke, Annabel Christ, Nina Liebe, Johannes Greiner, et al.overexpression clathrin-coated vesicles (1:100 rabbit anti-Clathrin Heavy Chain (P1663) in PBS with 5% BSA, #2410, Cell Signaling) were co-stained in this step. Afterwards cells were washed and incubated with fluorochrome-labeled

(9) Clathrin-associated AP-1 controls termination of STING signallingNatureOctober 1, 2022Liu Y, Xu P, Rivara S, Liu C, Ricci J, Ren X, Hurley JH, Ablasser A.immunoblot 1:1,000), rabbit monoclonal anti-clathrin heavy chain (P1663) (Cell Signaling Technology, 2410, immunoblot 1:500), mouse anti-clathrin heavy chain monoclonal antibody (X22) (Thermo Fisher Scientific,

(10) The ARSACS disease protein sacsin controls lysosomal positioning and reformation by regulating microtubule dynamicsThe Journal of biological chemistrySeptember 1, 2022Francis V, Alshafie W, Kumar R, Girard M, Brais B, McPherson PS.(ADI-SPA-815-F). Clathrin heavy chain antibody (WB-1:1000) was purchased from Cell Signaling Technology (#2410S). Anti RhoA antibody (WB: 1:2000) was purchased from Cell signaling Technology (#2117). Rabbit polyclonal

(11) Internalization and trafficking of CSPG-bound recombinant VAR2CSA lectins in cancer cellsScientific ReportsFebruary 23, 2022Chris Kedong Wang et al.membrane following standard methods. Membranes were probed with dilutions of primary antibodies CHC (Cat# 2410S) and Cav-1 (Cat# 3238S) from Cell Signaling, V5 (Cat# R96025) from ThermoFisher, and Actin (Cat# A2228-100UL)

(12) Delivering Antisense Oligonucleotides across the Blood-Brain Barrier by Tumor Cell-Derived Small Apoptotic BodiesAdvanced science (Weinheim, Baden-Wurttemberg, Germany)July 1, 2021Wang Y, Pang J, Wang Q, Yan L, Wang L, Xing Z, Wang C, Zhang J, Dong L.time points. The endocytosis‐dependent proteins clathrin (anti‐clathrin heavy chain P1663 antibody, 2410S, CST, USA) and caveolin‐1 (anti‐caveolin‐1 antibody, 3238S, CST, USA) were examined at 5 min after the

(13) Involvement of CASP9 (caspase 9) in IGF2R/CI-MPR endosomal transportAutophagyJune 1, 2021Han J, Goldstein LA, Hou W, Watkins SC, Rabinowich H.(hexosami nidase beta) sc-376781; IGF2R/CI-MPR Abcam ab124767 (staining and immunoblotting); COX4I1 4844 and CLTC 2410 from Cell Signaling Technology; and FLAG 3165 from Sigma. CTSD Abs included human-specific AF1014 Ab

(14) Intermittent fasting therapy promotes insulin sensitivity by inhibiting NLRP3 inflammasome in rat modelAnnals of palliative medicineMay 1, 2021Liang BJ, Liao SR, Huang WX, Huang C, Liu HS, Shen WZ.incubated with horseradish Ann Palliat Med 2021;10(5):5299-5309 | http://dx.doi.org/10.21037/apm-20-2410 5302 Liang et al. Intermittent fasting therapy inhibits NLRP3 inflammasome peroxidase (HRP)-conjugated

(15) Differences in surface chemistry of iron oxide nanoparticles result in different routes of internalizationBeilstein Journal of NanotechnologyMarch 23, 2021Barbora Svitkova, Vlasta Zavisova, Veronika Nemethova, Martina Koneracka, Miroslava Kretova, Filip Razga, Monika Ursinyova, Alena Gabelovanitrocellulose membranes the following primary antibodies were used: anti-Clathrin Heavy Chain (P1663) (#2410), anti-Caveolin-1 (#3238), anti-Phospho-Caveolin-1 (Tyr14) (#3251), anti-Dynamin I/II (#2342) (Cell Signaling Technology

(16) Engineering multifunctional metal/protein hybrid nanomaterials as tools for therapeutic intervention and high-sensitivity detectionâ€ Chemical ScienceJanuary 1, 2021Antonio Aires, David Maestro, Jorge Ruiz del Rio, Ana R. Palanca, Elena Lopez-Martinez, Irantzu Llarena, Kalotina Geraki, Carlos Sanchez-Cano, Ana V. Villar, Aitziber L. CortajarenaBansal, S. Rana, K. Datta, S. Chattopadhyay, M. Chawla-Sarkar and S. Sarkar, Cell. Signalling, 2015, 27, 2410–2424. 27 M. J. Pushie, I. J. Pickering, M. Korbas, M. J. Hackett and G. N. George, Chem. Rev., 2014

(17) A novel role for the late-onset Alzheimer’s disease (LOAD)-associated protein Bin1 in regulating postsynaptic trafficking and glutamatergic signalingMolecular PsychiatrySeptember 1, 2020Britta Schürmann et al.Synapsin mAb (CellSignaling Cat# 5297), PSD95 mAb (Neuromab Cat# 75–028), CHC pAb (CellSignaling Cat# 2410), Rab5 pAb (CellSignaling Cat# 2143), EEA1 pAb (CellSignaling Cat# 2411), Rab11 pAb (Sigma Cat# R5903),

(18) Cell Type- and Stimulation-Dependent Transcriptional Programs Regulated by Atg16L1 and Its Crohn's Disease Risk Variant T300AJournal of immunology (Baltimore, Md. : 1950)July 15, 2020Varma M, Kadoki M, Lefkovith A, Conway KL, Gao K, Mohanan V, Tusi BK, Graham DB, Latorre IJ, Tolonen AC, et al.1:1000); Lamp2 (Sigma L0668, 1:1000); Cathepsin C (Santa Cruz 74590, 1:500); Clathrin (Cell Signaling 2410, 1:1000). Immunoblotting Cells were lysed in standard lysis buffer [50 mM Tris-HCl pH 7.5, 150 mM

(19) A novel c-Kit/phospho-prohibitin axis enhances ovarian cancer stemness and chemoresistance via Notch3-PBX1 and β-catenin-ABCG2 signalingJournal of biomedical scienceMarch 13, 2020Fang CH, Lin YT, Liang CM, Liang SM.phospho-PHBT258 (11,588, SAB; Signalway Antibody), caveolin 1 (GTX100205, GeneTex), clathrin heavy chain (2410, Cell Signaling Technology), E-cadherin (20874–1-AP, Proteintech, Chicago, IL, USA), N-cadherin (GTX127345,

(20) Entry of Challenge Virus Standard (CVS) -11 into N2a cells via a clathrin-mediated, cholesterol-, dynamin-, pH-dependent endocytic pathwayVirology JournalDecember 1, 2019Jie Gao, Xinyu Wang, Mingxin Zhao, Enhua Liu, Ming Duan, Zhenhong Guan, Yidi Guo, Maolin ZhangNovus) (1:1000), GAPDH (1A6) mAb (MB001, Bioworld) (1:5000), Clathrin Heavy Chain (P1663) Antibody (#2410, Cell Signaling Technology) (1:500), Caveolin-1 Antibody (#3238, Cell Signaling Technology) (1:500). Immunofluorescence analysis

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Clathrin Heavy Chain (P1663) Antibody

Clathrin Heavy Chain (P1663) Antibody from Cell Signaling Technology

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Cell Signaling Technology

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Clathrin Heavy Chain (P1663) Antibody from Cell Signaling Technology

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