DDR1 (D1G6) XP ® Rabbit mAb 5583S from Cell Signaling Technology

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Genes & development
February 01 2018

Fig 1: DDR1−/− mammary tumors grow faster and are more basal and necrotic. (A) Primary tumor burden of PyMT/DDR1−/− mice compared with PyMT/DDR1+/+ and PyMT/DDR1+/− mice. PyMT/DDR1−/− mammary tumors grow faster than control tumors. Data are shown as mean ± SD. n = 4–7. (*) P < 0.02, one-way ANOVA and unpaired Student's t-test. (B) DDR1+/+ and DDR1+/− mammary tumors showed large epithelial clusters, and DDR1−/− tumors showed multiple small clusters and central necrosis (orange dots) by H&E staining. Representative photographs are shown (13 wk). n = 3. (C) The lengths of epithelial tumor clusters were measured. DDR1−/− primary tumors show small clusters by H&E staining. Data are shown as mean ± SD. n = 3. (*) P < 0.05; (**) P < 0.02, one-way ANOVA and unpaired Student's t-test. (D) Percentage of necrotic clusters out of small clusters (200–300 µm in length) was measured. Necrotic small clusters increased in DDR1−/− tumors. Data are shown as mean ± SD. n = 3. (*) P < 0.02, one-way ANOVA and unpaired Student's t-test. (E,F) Immunofluorescence of DDR1+/+ and DDR1−/− mammary tumor tissues using anti-keratin 8 (anit-K8) (green) and anti-phospho-histone H3 (phH3) (red) antibodies. (Blue) DAPI-stained nuclei. (E) Representative photographs are shown. PhH3+ cells were present mainly at the edge of the epithelial clusters. (F) The percentage of phH3+ cells per epithelial cell (K8+ and phH3+) shows that DDR1−/− mammary tumor cells are more proliferative. Data are shown as mean ± SD. n = 3. (*) P < 0.02, unpaired Student's t-test. (G) Immunofluorescence of DDR1+/+ and DDR1−/− mammary tumor tissues using anti-vimentin antibody (red) and DAPI-stained nuclei (blue). Representative photographs are shown. (H) Expression levels of vimentin were quantified by using ImageJ software. Vimentin levels increased in DDR1−/− epithelial clusters. Data are shown as mean ± SD. n = 4. (*) P < 0.05, unpaired Student's t-test. (I,J) Immunofluorescence of DDR1+/+, DDR1+/−, and DDR1−/− mammary tumor tissues using anti-E-cadherin (green) and anti-keratin 14 (anti-K14) (red) antibodies and DAPI-stained nuclei (blue). (I) K14+ basal cells were mainly at the edges of the epithelial clusters. Representative photographs are shown. (J) The ratio of K14+ basal cells per epithelial (E-cadherin+ and K14+) cell increased ([*] P < 0.02; [**] P < 0.05, one-way ANOVA and unpaired Student's t-test) (left panel) and expression of E-cadherin decreased ([**] P < 0.05, one-way ANOVA and unpaired Student's t-test) (right panel) in DDR1−/− epithelial clusters. Data are shown as mean ± SD. n = 3. (K,L) Immunofluorescence of the primary tumors was performed by using anti-K8 (green) and anti-DDR2 (red) antibodies. (Blue) DAPI-stained nuclei. (K) Representative photos of tumor epithelial clusters are shown. (L) The ratio of DDR2+ cells per epithelial cell increased significantly in DDR1−/− epithelial clusters. Data are shown as mean ± SD. n = 3. (*) P < 0.01, one-way ANOVA and unpaired Student's t-test. (M,N) Immunofluorescence of DDR1+/+, DDR1+/−, and DDR1−/− mammary tumor tissues was conducted by using anti-K8 (green) and anti-K14 (red) antibodies. (Blue) DAPI-stained nuclei. (M) Representative photos of tumor necrotic areas are shown. (N) K14+ basal cells, including K8+K14+ basal-like cells (yellow), significantly increased near necrotic fields of DDR1−/− mammary tumor tissues. Data are shown as mean ± SD. n = 3. (*) P < 0.05, one-way ANOVA and unpaired Student's t-test. (O) Immunofluorescence of DDR1−/− mammary tumor tissues using anti-E-cadherin (green) and anti-hypoxia-inducible factor α (anti-HIF1α) (red) antibodies and DAPI-stained nuclei (blue). (Right panel) The white dots represent a border between an epithelial and a necrotic field. HIF1α is expressed and localized near necrosis.
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Biology Open
August 09 2019

Fig 2: Disruption of rafts resulted in reduction of N-cadherin, catenins and DDR1 in the lipid raft fraction. Protein levels of N-cadherin, DDR1, a-catenin, ß-catenin, p120-catenin, flotillin-2, caveolin-1, GAPDH and ß-actin in total cell lysate (T) and fractions (1–9) after separation of cell lysate along a 40%/30%/5% sucrose gradient from DDR1+/+ (A), and DDR1-/- (B), VSMCs that were untreated or treated with 1 mM MßCD, followed by 1 mM of MßCD-cholesterol for 30 min or 1 h. Experiments were repeated three times.
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Biology Open
August 09 2019

Fig 3: Disruption of lipid rafts resulted in the reduction of N-cadherin contacts between cells. (A) Immunofluorescence of DDR1+/+ and DDR1-/- VSMCs treated with or without 1 or 2 units of cholesterol oxidase for 1 or 2 h, fixed and stained for N-cadherin (red; 610921 BD Biosciences). Nuclei are counterstained with Hoechst (blue). (B) Immunofluorescence of DDR1+/+ and DDR1-/- VSMCs treated with or without 1 mM of MßCD for 30 min, followed by 1 mM of MßCD-cholesterol for 30 min or 1 h, fixed and stained for N-cadherin (green; 610921 BD Biosciences).
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Biology Open
August 09 2019

Fig 4: Levels of N-cadherin and catenins were reduced in lipid rafts from DDR1-/- VSMCs. (A) Immunofluorescence of DDR1+/+ VSMCs stained for N-cadherin (red; 610921 BD Biosciences) and GM1 (green; Alexa 488 cholera toxin B, C34775 Life Technologies). Nuclei are counterstained with Hoechst (blue). (B) Protein levels of N-cadherin, DDR1, a-catenin, ß-catenin, p120-catenin, flotillin-2, caveolin-1, GAPDH, and ß-actin in total cell lysate (T), and fractions (1–9) after separation of cell lysate from DDR1+/+ and DDR1-/- VSMCs along a 40%/30%/5% sucrose gradient. (C) Protein levels of N-cadherin, DDR1, a-catenin, ß-catenin, p120-catenin, flotillin-2, caveolin-1, GAPDH and ß-actin in total cell lysate and fractions 3–5. Blots were spliced to compose the image, but all samples were immunoblotted and developed simultaneously. (D) Relative fold changes of N-cadherin, a-catenin, ß-catenin, p120-catenin and ß-actin (n=3) in lipid raft fractions of DDR1+/+ and DDR1-/- VSMCs normalized to caveolin-1, expressed relative to DDR1+/+ VSMCs. Data are plotted as mean±s.e.m. Student’s t-test was performed. * indicates statistical significance of P<0.05.
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Molecular metabolism
September 01 2020

Fig 5: DDR1 deficient mice have improved metabolic function after 12 weeks on HFD. (A) Liver hematoxylin and eosin (H&E) and Oil Red O (ORO) staining obtained from Ddr1+/+ and Ddr1-/- mice fed HFD. (B) Insulin staining on pancreatic sections obtained from Ddr1+/+ and Ddr1-/- mice. (C) Pancreatic islet area was determined in Ddr1+/+ and Ddr1-/- mice fed normal chow diet (CF) or HFD for 12 weeks (n = 6). (D-E) Oral glucose tolerance test (GTT) was performed in Ddr1+/+ and Ddr1-/- mice before (D; n = 8) and after 12 weeks on HFD (E; n = 8). (F-G) Insulin tolerance test (ITT) was performed in Ddr1+/+ and Ddr1-/- mice before (F; n = 7) and after 12 weeks on HFD (G; n = 7). (H) Fasting circulating insulin levels were determined in Ddr1+/+ and Ddr1-/- mice fed HFD (n = 6). Statistical analysis was performed by 2-way ANOVA with Bonferroni post-hoc test (D, E, F, and G), and student's t-test (C and H). The values are mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.

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Cell Signaling Technology for
DDR1 (D1G6) XP ® Rabbit mAb

The DDR1 (D1G6) XP ® Rabbit mAb Antibody from Cell Signaling Technology is a Rabbit Monoclonal antibody to DDR1. This antibody recognizes Homo Sapiens, Human, Mouse, Rat, and Non-Human Primate antigen. The DDR1 (D1G6) XP ® Rabbit mAb Antibody has been validated for the following applications: Precipitation, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry - fixed, Immunoprecipitation, and Western Blot.

Description

DDR1 (D1G6) XP® Rabbit mAb detects endogeneous levels of total DDR1 protein

Product Specs
ItemDDR1 (D1G6) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number5583S
Quantity100 µl
ApplicationsW, IP, IHC-P, IF-IC
ReactivityH, M, R, Mk
TargetDDR1
HostRabbit
NCBI Gene AliasesCAK, CD167, DDR, EDDR1, HGK2, MCK10, NEP, NTRK4, PTK3, PTK3A, RTK6, TRKE
ClonalityMonoclonal
NCBI Full Gene Namediscoidin domain receptor tyrosine kinase 1
Research AreaRTK
IsotypeIgG
CloneD1G6
Molecular Weight125

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(1) Prognostic significance of TM4SF1 and DDR1 expression in epithelial ovarian cancerOncology LettersAugust 30, 2023Zhijiong Huang, Hongyu Yao, Zhijun Yangincubated in rabbit anti-TM4SF1 (1:1,000; cat. no. ab113504; Abcam) or rabbit anti-DDR1 (1:3,000; cat. no. 5583; Cell Signaling Technology, Inc.) overnight at 4°C, biotin-labeled goat anti-mouse/rabbit IgG for 1

(2) Biomimetic nanoparticles drive the mechanism understanding of shear-wave elasticity stiffness in triple negative breast cancers to predict clinical treatmentBioactive materialsApril 1, 2023Zheng D, Zhou J, Qian L, Liu X, Chang C, Tang S, Zhang H, Zhou S.Beyotime; MAB1420-SP, R&D Systems), Phospho-Smad3 (Ser423/425) antibody (#9520, CST), DDR1 antibody (#5583, CST), HIF-1α antibody (#36169, CST), GAPDH (#5174, CST). 5.9 Cellular immunofluorescence The cells

(3) Cell Competition Shapes Metastatic Latency and RelapseCancer discoveryJanuary 9, 2023Kim K, Huang H, Parida PK, He L, Marquez-Palencia M, Reese TC, Kapur P, Brugarolas J, Brekken RA, Malladi S.Vitronectin/S-protein (Abcam Cat# ab13413, RRID:AB_2216293, 1:500), DDR1 (Cell Signaling Technology Cat# 5583, RRID:AB_10694842, 1:1000), p-DDR1 Tyr792 (Cell Signaling Technology Cat# 11994, RRID:AB_2797793, 1:1000),

(4) DDR1 functions as an immune negative factor in colorectal cancer by regulating tumor-infiltrating T cells through IL-18Cancer scienceNovember 1, 2022Duan X, Xu X, Zhang Y, Gao Y, Zhou J, Li J.incubated with primary antibodies against PD‐L1 (Proteintech, #14‐5983‐82), PD‐1 (CST, #84651), DDR1 (CST, #5583), CD4 (Abcam, #ab183685) and CD8α (CST, #989941) overnight at 4°C. After rinsing with PBS, the slides

(5) Mechanical Confinement and DDR1 Signaling Synergize to Regulate Collagenâ€Induced Apoptosis in Rhabdomyosarcoma CellsAdvanced ScienceOctober 1, 2022Jordi Gonzalezâ€Molina, Katharina Miria Kirchhof, Bhavik Rathod, Lidia Moyanoâ€Galceran, Maria Calvoâ€Noriega, Georgia Kokaraki, Astrid BjÃ¸rkÃ¸y, Monika Ehnman, Joseph W. Carlson, Kaisa Lehtiused for western blot were pDDR1 (Tyr792; #11 994; Cell Signaling Technology; 1:500), DDR1 (D1G6; #5583; Cell Signaling Technology; 1:1000), β‐actin (C‐4; sc‐47778; Santa Cruz Biotechnology; 1:2000), MMP14

(6) Oncogenic collagen I homotrimers from cancer cells bind to α3β1 integrin and impact tumor microbiome and immunity to promote pancreatic cancerCancer cellAugust 8, 2022Chen Y, Yang S, Tavormina J, Tampe D, Zeisberg M, Wang H, Mahadevan KK, Wu CJ, Sugimoto H, Chang CC, et al.1:2000), AKT (4691, Cell Signaling, 1:1000), phospho-AKT Ser473 (4060, Cell Signaling, 1:500), DDR1 (5583, Cell Signaling, 1:500), phospho-DDR1 Tyr792 (11994, Cell Signaling, 1:500), ERK1/2 (9102, Cell Signaling,

(7) DDR1 promotes LoVo cell proliferation by regulating energy metabolismActa Biochimica et Biophysica SinicaMay 25, 2022Bin Xiong, Zehui Xie, Feixue Song, Huiling Chen, Xiaojuan Wang, Zhengxu Jin, Tiyun Han, Yi Li, Dekui Zhangstandard protocols. Briefly, the slides were incubated with anti-DDR1 antibody (D1G6 XP ® Rabbit mAb 5583; Cell Signaling Technology, Danvers, USA) overnight at 4°C. The slides were then incubated with an HRP-conjugated

(8) The Collagen Receptor Discoidin Domain Receptor 1b Enhances Integrin β1-Mediated Cell Migration by Interacting With Talin and Promoting Rac1 ActivationFrontiers in Cell and Developmental BiologyMarch 3, 2022Corina M. Borza et al.antibodies were used: DDR1 (Santa Cruz SC-532, 1:4,000 for WB and 1:2000 for ELISA; Cell Signaling #5583, clone D1G6, 1:2000 for WB and 1:1,000 for IF; mAb 7A9 (Carafoli et al., 2012), 1:200 for FACS; R&D

(9) Discoidin Domain Receptor 1 Expression in Colon Cancer: Roles and Prognosis ImpactCancers (Basel)February 13, 2022Kaouther Ben Arfi et al.performed using DDR1 (D1G6) XP® Rabbit mAb, rabbit Monoclonal antibody (1/100, Cell Signaling ref: #5583) after heat-induced epitope retrieval in citrate pH 6 buffer (95 °C, 40 min) and overnight antibody

(10) DDR1 contributes to kidney inflammation and fibrosis by promoting the phosphorylation of BCR and STAT3JCI insightFebruary 8, 2022Borza CM, Bolas G, Bock F, Zhang X, Akabogu FC, Zhang MZ, de Caestecker M, Yang M, Yang H, Lee E, et al.were incubated with the following primary antibodies from Cell Signaling Technology: DDR1 (catalog 5583), pY513-DDR1 (catalog 14531), pY792-DDR1 (catalog 11994), BCR (catalog 3902), pY177-BCR (catalog 3901),

(11) LncRNA SNHG12 promotes the malignant progression of melanoma by targeting miR-199bAnnals of Translational MedicineFebruary 1, 2022Yijie Xie, Guangxiong Chenanti-Paxillin antibody (ab32084, Abcam), anti-Jagged1 antibody (ab7771, Abcam), and anti-DDR1 antibody (#5583, Cell Signaling Technology). GAPDH was used as the protein internal control. Statistical analysis SPSS

(12) Breast tissue regeneration is driven by cell-matrix interactions coordinating multi-lineage stem cell differentiation through DDR1Nature communicationsDecember 10, 2021Rauner G, Jin DX, Miller DH, Gierahn TM, Li CM, Sokol ES, Feng YX, Mathis RA, Love JC, Gupta PB, et al.antibody, washed and stained with DAPI or Hoechst 34580. Primary antibodies used in this study were: DDR1 (5583, Cell Signaling Technology, clone D1G6, 1:100), E-Cadherin (ab1416, Abcam, Clone HECD-1, 1:100), JAG1

(13) Expression and subcellular localization of Discoidin Domain Receptor 1 (DDR1) define prostate cancer aggressivenessCancer Cell InternationalDecember 1, 2021R. Daniel Bonfil, Wei Chen, Semir Vranic, Anjum Sohail, Dongping Shi, Hyejeong Jang, Hyeong-Reh Kim, Marco Prunotto, Rafael FridmanDanvers, MA. After stripping, the membranes were re-probed with total anti-DDR1 (D1G6) rabbit mAb (Cat. # 5583, CST), which recognizes the C-terminal region of DDR1. The membrane was also reprobed for presence of

(14) Tumour DDR1 promotes collagen fibre alignment to instigate immune exclusionNatureNovember 1, 2021Sun X, Wu B, Chiang HC, Deng H, Zhang X, Xiong W, Liu J, Rozeboom AM, Harris BT, Blommaert E, et al.transferred to membrane for immunoblotting. Primary antibodies are: anti-DDR1 (dilution: 1:1,000; CST, 5583S), anti-DDR2 (dilution: 1:1,000; Sigma, MABT322), anti-GAPDH (dilution:1:5,000; CST, 2118S), anti-β-actin

(15) The KRAS-regulated kinome identifies WEE1 and ERK coinhibition as a potential therapeutic strategy in KRAS-mutant pancreatic cancerJournal of Biological ChemistryNovember 1, 2021J. Nathaniel Diehl et al.and MG-132 (M7449). The following antibodies were obtained from Cell Signaling Technology: anti-DDR1 (5583S), anti-PYK2 (3090S), anti-STAT3 (9139S), antiphosphorylated STAT3 Tyr705 (9145S), anti-WEE1 (13084S),

(16) DDR1-induced neutrophil extracellular traps drive pancreatic cancer metastasisJCI insightSeptember 8, 2021Deng J, Kang Y, Cheng CC, Li X, Dai B, Katz MH, Men T, Kim MP, Koay EA, Huang H, et al.tris-buffered saline with 0.1% Tween 20 for 1 hour. It was then hybridized with DDR1 (D1G6; Cell Signaling, 5583), SYK (Cell Signaling, 2712), phospho-SYK (Cell Signaling, 2710), PKCθ (Cell Signaling, 13643), phospho-PKCθ

(17) Promotion of cholangiocarcinoma growth by diverse cancer-associated fibroblast subpopulationsCancer cellJune 14, 2021Affo S, Nair A, Brundu F, Ravichandra A, Bhattacharjee S, Matsuda M, Chin L, Filliol A, Wen W, Song X, et al.(1:1000 Cell Signaling, #9272), YAP/TAZ (1:1000, Cell Signaling, #8418), DDR1 (1:1000, Cell Signaling, #5583) followed by incubation with horseradish peroxidase-conjugated secondary antibody against rabbit IgG

(18) Molecular Analysis of ZNF71 KRAB in Non-Small-Cell Lung CancerInternational journal of molecular sciencesApril 4, 2021Ye Q, Mohamed R, Dukhlallah D, Gencheva M, Hu G, Pearce MC, Kolluri SK, Marsh CB, Eubank TD, Ivanov AV, et al.NBP1-77971), beta-Catenin (6B3, Cell Signaling, Danvers, MA, USA; 9582), DDR1 (D1G6, Cell Signaling, 5583), pan-Cytokeratin (C11, Santa Cruz, Dallas, TX, USA; sc-8018), Actin (I-19, Santa Cruz, sc-1616), PCNA (eBioSciences,

(19) Discoidin domain receptor 1-deletion ameliorates fibrosis and promotes adipose tissue beiging, brown fat activity, and increased metabolic rate in a mouse model of cardiometabolic diseaseMolecular metabolismSeptember 1, 2020Lino M, Ngai D, Liu A, Mohabeer A, Harper C, Caruso LL, Schroer SA, Fu F, McKee T, Giacca A, et al.Cell Signaling Technology). Antibodies were obtained from Cell Signaling Technology unless otherwise specified: DDR1 (5583); UCP-1 (ab10983; Abcam); phospho-HSL (4139); HSL (4107); FAS (3180); Perilipin (9349); PPARγ (2435);

(20) DDR1 (Discoidin Domain Receptor-1)-RhoA (Ras Homolog Family Member A) Axis Senses Matrix Stiffness to Promote Vascular CalcificationArteriosclerosis, Thrombosis, and Vascular BiologyJuly 1, 2020David Ngai, Marsel Lino, Katheryn E. Rothenberg, Craig A. Simmons, Rodrigo Fernandez-Gonzalez, Michelle P. Bendeckcocktail tablet (04693159001; Roche), 1 μM PMSF, and 200 nM NaF (sodium fluoride). DDR1 antibody (1:100; 5583) was added to 500 μg protein and incubated overnight at 4°C with rotation. Thirty microliters of 50%

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Genes & development

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Molecular metabolism

DDR1 (D1G6) XP ® Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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DDR1 (D1G6) XP ® Rabbit mAb from Cell Signaling Technology

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