beta-Actin (13E5) Rabbit mAb (Biotinylated) 5057S from Cell Signaling Technology

Product Specs
Itembeta-Actin (13E5) Rabbit mAb (Biotinylated)
CompanyCell Signaling Technology
Price $450.00 Inquire
Catalog Number5057S
Quantity100 µl
ApplicationsW
ReactivityH, M, R, Mk, B, Pg
Targetbeta-Actin
HostRabbit
NCBI Gene AliasesBRWS1, PS1TP5BP1
Conjugate/TagBiotin
ClonalityMonoclonal
NCBI Full Gene Nameactin beta
Research AreaCytoskeletal Signaling
IsotypeIgG
Clone1300000
Molecular Weight45

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Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Porcine cGAS-STING signalling induced apoptosis negatively regulates STING downstream IFN response and autophagy via different mechanismsVirulenceMay 1, 2025Nengwen Xia, Anjing Liu, Hongjian Han, Sen Jiang, Qi Cao, Jia Luo, Jiajia Zhang, Weilin Hao, Ziyan Sun, Nanhua Chen, et al.(Asp175) (9664S), TBK1 (3504S), p-TBK1 (5483S), IRF3 (11904S), FLAG (14793), GFP (2956) and β-actin (5057) were acquired from Cell Signaling Technology (Boston, MA, USA). The phosphorylated-IRF3 (Ser396) rabbit

(2) Identification of a New Conserved Antigenic Epitope by Specific Monoclonal Antibodies Targeting the African Swine Fever Virus Capsid Protein p17Veterinary SciencesDecember 13, 2024Nengwen Xia, Qi Cao, Anjing Liu, Jiajia Zhang, Hongjian Han, Jun Jiao, Ping He, Ziyan Sun, Zijian Xu, Wanglong Zheng, et al.Ltd.(Luoyang, China) The RFP mAb (ab185921) was bought from Abcam (Cambridge, UK). The anti-β-actin mAb (5057) was purchased from Cell Signaling Technology (Boston, MA, USA). The anti-PRRSV N mAb, anti-PEDV N mAb,

(3) DNA polymerase η is regulated by mutually exclusive mono-ubiquitination and mono-NEDDylationbioRxivOctober 17, 2024Natália Cestari Moreno, Emilie J. Korchak, Marcela Teatin Latancia, Dana A. D’Orlando, Temidayo Adegbenro, Irina Bezsonova, Roger Woodgate, Nicholas W. Ashton#3724S), Rb-α-PCNA (Cell Signaling #D5C7P), Ms-α-PCNA (Santa Cruz #K3023), Rb-α-actin (Cell Signaling #5057S), Ms-α-H3 (Cell Signaling #8173S). Primary antibodies were detected using IRDye 680RD or 800CW-conjugated

(4) E3 ubiquitin ligase RNF2 protects polymerase ι from destabilizationBiochimica et Biophysica Acta (BBA) - Molecular Cell ResearchJune 1, 2024Mikolaj Fedorowicz, Agnieszka Halas, Matylda Macias, Ewa Sledziewska-Gojska, Roger Woodgate, Justyna McIntyreab6160), anti-GAPDH (Thermo Fisher Scientific, cat. no. PA1–987), anti-β-Actin (Cell Signaling, cat. no. 5057), anti LC3B (Cell Signaling, cat. no. 2775), anti-p53 (Santa Cruz, cat. no. sc-98), anti-Polι (Invitrogen,

(5) SARS-CoV-2 protein ORF8 limits expression levels of Spike antigen and facilitates immune evasion of infected host cellsThe Journal of biological chemistryAugust 1, 2023Kim IJ, Lee YH, Khalid MM, Chen IP, Zhang Y, Ott M, Verdin E.anti-COX4, Cell Signaling, Cat #: 4850, 1:2,000 dilution) (rabbit anti-β-actin, Cell Signaling, Cat #: 5057, 1:5,000 dilution) (rabbit anti-Calreticulin, Cell Signaling, Cat #: 12238, 1:2,000 dilution) (mouse

(6) Manganese Mediates Its Antiviral Functions in a cGAS-STING Pathway Independent MannerVirusesFebruary 28, 2023Sun S, Xu Y, Qiu M, Jiang S, Cao Q, Luo J, Zhang T, Chen N, Zheng W, Meurens F, et al.Shanghai, China), TBK1 (3504S, CST, Boston, MA, USA), p-TBK1 (5483S, CST, Boston, MA, USA) and β-actin (5057, CST, Boston, MA, USA). Secondary antibody HRP-conjugated goat anti-mouse or rabbit IgG (TransGen Biotech,

(7) Establishment of anti-DKK3 peptide for the cancer control in head and neck squamous cell carcinoma (HNSCC)Cancer cell internationalNovember 15, 2022Katase N, Nishimatsu SI, Yamauchi A, Okano S, Fujita S.Cell Signaling Technology, Inc., Danvers, MA, USA), p-Akt (Ser473) (9271S, Cell Signaling Technology), and β-actin (5057S, Cell Signaling Technology) at 1:500–1:1,000 dilution. The membranes were then rinsed in TBST, followed

(8) HILPDA Uncouples Lipid Droplet Accumulation in Adipose Tissue Macrophages from Inflammation and Metabolic DysregulationSSRN Electronic JournalMarch 31, 2022Xanthe A.M.H. van Dierendonck, Montserrat A. de la Rosa Rodriguez, Anastasia Georgiadi, Frits Mattijssen, Wieneke Dijk, Michel van Weeghel, Rajat Singh, Jan Willem Borst, Rinke Stienstra, Sander KerstenRRID:AB_2297252 G0S2 Santa Cruz Biotechnology Cat# sc-133424; RRID:AB_2107211 ACTIN Cell Signaling Cat# 5057; RRID: AB_10694076 TUBULIN Cell Signaling Cat# 2146; RRID: AB_2210545 HSP90 Cell Signaling Cat#

(9) Triglyceride breakdown from lipid droplets regulates the inflammatory response in macrophagesProceedings of the National Academy of Sciences of the United States of AmericaMarch 22, 2022van Dierendonck XAMH, Vrieling F, Smeehuijzen L, Deng L, Boogaard JP, Croes CA, Temmerman L, Wetzels S, Biessen E, Kersten S, et al.9145T; Cell Signaling Technology); phospho–c-Jun (Ser63; 9261S; Cell Signaling Technology); ACTIN (5057; RRID: AB_10694076; Cell Signaling Technology); or HSP90 (4874S; RRID: AB_2121214; Cell Signaling Technology).

(10) Porcine cGAS-STING signaling induced autophagy inhibits STING downstream IFN and apoptosisFrontiers in immunologyJanuary 1, 2022Xia N, Zheng W, Jiang S, Cao Q, Luo J, Zhang J, Xu Y, Sun S, Zhang K, Chen N, et al.(Asp175) (9664S), TBK1 (3504S), p-TBK1 (5483S), IRF3 (11904S), FLAG (14793), GFP (2956) and β-actin (5057) were acquired from Cell Signaling Technology (Boston, MA, USA). The phosphorylated-IRF3 (Ser396) rabbit

(11) African Swine Fever Virus Structural Protein p17 Inhibits Cell Proliferation through ER Stress—ROS Mediated Cell Cycle ArrestVirusesDecember 24, 2020Nengwen Xia et al.purchased from Becton Dickinson Company (BD; Franklin Lakes, NJ, USA). The antibodies against β-actin (5057), Cyclin A2 (91500), Cyclin B1 (12231), Cyclin E1 (20808), Geminin (52508), GPR78 (3177), PERK (5683),

(12) Medium-Intensity Treadmill Exercise Exerts Beneficial Effects on Bone Modeling Through Bone Marrow Mesenchymal Stromal CellsFrontiers in Cell and Developmental BiologyNovember 24, 2020Lingli Zhang, Yu Yuan, Wei Wu, Zhongguang Sun, Le Lei, Jing Fan, Bo Gao, Jun Zoup44/42 MAPK (CST, #9102), phospho-p38 MAPK (CST, #9211), p38 MAPK (CST, #9212), and β-Actin (CST, #5057). The molecular weight and gray value of the target strip were analyzed by the Image J system. Serum

(13) Disruption of FGF Signaling Ameliorates Inflammatory Response in Hepatic Stellate CellsFrontiers in cell and developmental biologyJanuary 1, 2020Wang C, Li Y, Li H, Zhang Y, Ying Z, Wang X, Zhang T, Zhang W, Fan Z, Li X, et al.patients with chronic obstructive pulmonary disease. Mol. Med. Rep. 13, 4266–4272. doi: 10.3892/mmr.2016. 5057 McKeehan, W. L., Wang, F., and Luo, Y. (2009). "The fibroblast growth factor (FGF) signaling complex,”

(14) Miro1 Marks Parkinson's Disease Subset and Miro1 Reducer Rescues Neuron Loss in Parkinson's ModelsCell metabolismDecember 3, 2019Hsieh CH, Li L, Vanhauwaert R, Nguyen KT, Davis MD, Bu G, Wszolek ZK, Wang X.anti-Miro1 (ARP44818_P050, Aviva Systems Biology) at 1:1000, or biotinylated rabbit anti-β-actin (#5057S, Cell Signaling Technology) at 1:500, in 100 μl diluent (1% BSA in PBS, pH=7.3) for 2 hrs. Next, plates

(15) DKK3 knockdown confers negative effects on the malignant potency of head and neck squamous cell carcinoma cells via the PI3K/Akt and MAPK signaling pathwaysInternational journal of oncologyMarch 1, 2019Katase N, Nishimatsu SI, Yamauchi A, Yamamura M, Fujita S.domain‑containing mTOR‑interacting protein (DEPTOR) (1:1,000; cat. no. 11816S), β‑actin (1:50,000; cat. no. 5057S) (Cell Signaling Technology, Inc. Danvers, MA, USA) and p‑PI3K p85 (Y467)/p55 (Y199) (1:1,000; cat.

(16) Butyrate decreases its own oxidation in colorectal cancer cells through inhibition of histone deacetylasesOncotargetJune 5, 2018Anna Han, Natalie Bennett, Bettaieb Ahmed, Jay Whelan, Dallas R. Donohoe

(17) Synergistic effects of fibroblast growth factor-2 and bone morphogenetic protein-2 on bone inductionMolecular medicine reportsOctober 1, 2017Song R, Wang D, Zeng R, Wang J.catalog no. 9252), anti-p38 (cat. no. 9212), p-p38 (catalog no. 9215) and anti-β-actin (catalog no. 5057), and mouse anti-extracellular signal-regulated kinase (ERK) 1/2 (catalog no. 4696), p-ERK1/2 (catalog

(18) Diverse stimuli engage different neutrophil extracellular trap pathwayseLifeJune 2, 2017Elaine F Kenny, Alf Herzig, Renate Krüger, Aaron Muth, Santanu Mondal, Paul R Thompson, Volker Brinkmann, Horst von Bernuth, Arturo ZychlinskyMotif: 39164, discontinued) an anti-cleaved Caspase-3 antibody (9661, RRID:AB_2341188), anti-β-actin (5057S, RRID:AB_10694076) or anti-GAPDH (all Cell Signaling Technology: 5014S RRID:AB_10693448) and anti-rabbit

(19) New Insights into Non-Avian Dinosaur Reproduction and Their Evolutionary and Ecological Implications: Linking Fossil Evidence to Allometries of Extant Close RelativesPLoS ONEAugust 21, 2013Jan Werner, Eva Maria Griebeler(kg),CS,CST,CSC,AEN,CY,CSB,AEN,CY lambeosaurine,2390,4.737,22,3.5,9.9,10.3,0.5,23.2,25.7,1.2 lambeosaurine,3344,4.737,22,4.4,12.6,13.1,0.6,29.5,32.7,1.5 lambeosaurine,5057,4.737,22,6.0,16.9,17.7,0.8,39.7,44.1,2.0 Maiasaura peeblesorum,1500,1.023,16,11.6,32.9,34.0,2.1,77.1,84.8,5.3 Maiasaura

(20) Effects of icotinib, a novel epidermal growth factor receptor tyrosine kinase inhibitor, in EGFR-mutated non-small cell lung cancerOncology ReportsMarch 22, 2012Guangdie Yang, Yinan Yao, Jianya Zhou, Qiong Zhao(Thr202/Tyr 204, CST-4377), anti-Akt (CST-9272), anti-phospho-Akt (Ser473, CST-9272), anti‑β-actin (13E5, CST-5057) (all of antibodies were purchased from Cell Signaling Technology, Danvers, MA, USA). Post-incubation with

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beta-Actin (13E5) Rabbit mAb (Biotinylated)

beta-Actin (13E5) Rabbit mAb (Biotinylated) from Cell Signaling Technology

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Cell Signaling Technology

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beta-Actin (13E5) Rabbit mAb (Biotinylated) from Cell Signaling Technology

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