Rabbit (DA1E) mAb IgG XP ® Isotype Control (Sepharose ® Bead Conjugate) 3423S from Cell Signaling Technology

Product Specs
ItemRabbit (DA1E) mAb IgG XP ® Isotype Control (Sepharose ® Bead Conjugate)
CompanyCell Signaling Technology
Price $170.00 Inquire
Catalog Number3423S
Quantity400 µl
ApplicationsIP
Conjugate/TagSepharose ® Bead
IsotypeIgG
CloneDA1E
HostRabbit
ClonalityMonoclonal
Research AreaRelated Products

Add to Compare List

Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) U2AF1 modulates alternative exon selection and guards zygotic splicing activation in mouse preimplantation embryogenesisCellular and Molecular Life SciencesApril 16, 2026Zhi-Yan Jiang, Xuan Wu, Meng-Yan Jia, Lu Chen, Yu-Qi Liu, Heng-Yu Fan, Yin-Li ZhangU2AF1 antibody, as listed in Table S7. Rabbit (DA1E) mAb IgG XP Isotype (Cell Signaling Technology, 3423) was applied as the control. For UHM-lacking U2AF1-interacting RNAs, lysates of FGOs microinjected with

(2) NK cell-derived GZMB (granzyme B) suppresses glioblastoma radioresistance by blocking SDC1-mediated autophagosome maturationAutophagyJanuary 9, 2026Jingze Yan, Ruishen Feng, Qin Qin, Gefenqiang Shen, Meng Tian, Sunkai Ling, Shu Zhou, Fei Li, Xiaojie Xia, Yuandong Cao, et al.incubated with 1 μg anti-TGM2, anti-STX17 antibody, or anti-IgG antibody (Cell signaling Technology, 3423; 1:100) for 18 h followed by addition of 20 μl protein A/G plus agarose beads (Santa Cruz Biotechnology,

(3) FOSL1-PRMT1 transcriptional-epigenetic circuit promotes glioblastoma radioresistance via calcyphosine-mediated DNA repair and invasionMol BiomedDecember 22, 2025Yating Zhang, Jiajia Tian, Shuai Wu, Yiting Zhou, Zhongyuan Bao, Yi Zhu, Peng Wang, Zixiang Liu, Pengpeng Li, Zhenxing Tao, et al.supernatant was incubated with 1 µg of either anti-FOSL1/-PRMT1 antibody or anti-IgG antibody (Cat# 3423, Cell Signaling Technology, USA, diluted 1:100) for a duration of 16 h. Following incubation, 40 μL

(4) METTL14-mediated m6A modification of DUSP6 mRNA participating in postoperative cognitive dysfunction due to sevoflurane anesthesiaThe Journal of Physiological SciencesNovember 1, 2025Shengfeng Deng, Guo Mu, Jun Li, Xuan Yu, Qiang Li, Bin Lubetween METTL14 and DUSP6. Cells were lysed, and the extracted proteins were incubated with IgG (CST, #3423, 1: 20) or DUSP6 (CST, #50945, 1: 200) antibodies at 4°C for 1 h. Subsequently, RNA was isolated to

(5) Myristoylated Eepd1 Enhances Lipolysis and Thermogenesis through PKA Activation to Combat ObesityNat CommunJanuary 14, 2025Suzhen Chen, Yanping Wang, Qian Zhou, Qiqi Qian, Quanxin Jiang, Chuchu Liu, Yan Liu, Peihui Zhou, Jie Xiong, Yao Zhang, et al.followed by washing in cold IP buffer. Normal mouse or rabbit IgG (Cell Signaling Technology, 3420 or 3423) was used as a negative control. The immunocomplexes were collected and subjected to immunoblotting

(6) PCBP1/2 and TDP43 Function as NAT10 Adaptors to Mediate mRNA ac4C Formation in Mammalian CellsAdvanced ScienceDecember 1, 2024Zhi‐Yan Jiang, Yu‐Ke Wu, Zuo‐Qi Deng, Lu Chen, Yi‐Min Zhu, Yuan‐Song Yu, Hong‐Bo Wu, Heng‐Yu FanFast Flow beads (GE Healthcare, Chicago, IL, USA) for 1 h at 25 °C. Rabbit (DA1E) mAb IgG XP Isotype (3423; Cell Signaling Technology, Danvers, MA, USA) was used as the control. After centrifugation, the lysates

(7) ERRFI1 exacerbates hepatic ischemia reperfusion injury by promoting hepatocyte apoptosis and ferroptosis in a GRB2-dependent mannerMolecular MedicineJune 11, 2024Hang Zhao, Huizi Maoimmunoprecipitated with GRB2 antibody (ab32037, Abcam) at 4℃ overnight. Rabbit IgG control antibody (#3423; Cell Signaling Technology, Danvers, MA, USA) was used as a negative control. The immunocomplex was

(8) CKLF1, transcriptionally activated by FOXC1, promotes hypoxia/reoxygenation‑induced oxidative stress and inflammation in H9c2 cells by NLRP3 inflammasome activationExperimental and Therapeutic MedicineDecember 11, 2023Yinfeng Jia, Jiansheng Panat 4˚C with anti-FOXC1 (cat. no. ab227977; 1:50; Abcam) or negative control IgG antibodies (cat. no. 3423; 1:20; Cell Signaling Technology). The DNA isolated through ChIP reactions was evaluated using PCR as

(9) TBC1D15 deficiency protects against doxorubicin cardiotoxicity via inhibiting DNA-PKcs cytosolic retention and DNA damageActa Pharmaceutica Sinica BDecember 1, 2023Wenjun Yu, Haixia Xu, Zhe Sun, Yuxin Du, Shiqun Sun, Miyesaier Abudureyimu, Mengjiao Zhang, Jun Tao, Junbo Ge, Jun Ren, et al.centrifugation, supernatant fractions were subjected to immunoprecipitation with monoclonal IgG (1:1000, 3423, Cell Signaling) and monoclonal Flag antibodies (1:500, 70569, Sigma–Aldrich) incubated on protein-A/G

(10) MATN3 delivered by exosome from synovial mesenchymal stem cells relieves knee osteoarthritis: Evidence from in vitro and in vivo studiesJournal of orthopaedic translationJuly 1, 2023Long L, Zou G, Cheng Y, Li F, Wu H, Shen Y.remaining supernatant was supplemented with IL-17A (# 13838, 1:200, CST, Danvers, MA, USA) or IgG (# 3423, 1:20, CST) antibodies and incubated overnight at 4 °C. Next, after adding 20 μL of protein A/G-agarose

(11) β-arrestin1 regulates astrocytic reactivity via Drp1-dependent mitochondrial fission: implications in postoperative deliriumJournal of neuroinflammationMay 11, 2023Hua F, Zhu H, Yu W, Zheng Q, Zhang L, Liang W, Lin Y, Xiao F, Yi P, Xiong Y, et al.#sc-376447), or mouse IgG (Cell Signaling Technology, #3420) or rabbit IgG (Cell Signaling Technology, #3423) overnight, followed by incubation with protein A/G-agarose beads (Santa Cruz, #sc-2003) for another

(12) SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomesAutophagyMarch 1, 2023Zheng W, Chen Q, Liu H, Zeng L, Zhou Y, Liu X, Bai Y, Zhang J, Pan Y, Shao C.anti-SDC1/-LC3/-TGM2/-STX17/-EPG5/-Flag antibody, or anti-IgG antibody (Cell signaling Technology, 3423; 1:100) for 16 h followed by addition of 20 μl protein A/G plus agarose beads (Santa Cruz Biotechnology,

(13) A mass spectrometry-based approach for the identification of Kpnβ1 binding partners in cancer cellsScientific reportsNovember 23, 2022Okpara MO, Hermann C, van der Watt PJ, Garnett S, Blackburn JM, Leaner VD.of intracellular HeLa protein. A rabbit (DA1E) mAb IgG isotype control (Cell Signaling technology, #3423) that is not directed against any known antigen was included, together with protein A agarose beads

(14) Ferroportin-dependent ferroptosis induced by ellagic acid retards liver fibrosis by impairing the SNARE complexes formationRedox biologyOctober 1, 2022Li L, Wang K, Jia R, Xie J, Ma L, Hao Z, Zhang W, Mo J, Ren F.immunoprecipitated with VAMP2, syntaxin 4, SNAP23 antibodies at 4 °C overnight. Rabbit IgG control antibody (#3423, CST; 1:50) was used as a negative control. Immunoprecipitates were pulled down with Protein PLUS A/G

(15) Puerarin ameliorates acute lung injury by modulating NLRP3 inflammasome-induced pyroptosisCell death discoveryAugust 18, 2022Cai D, Zhao Y, Yu F.with 2 μg antibody to IKZF1 (#5443, 1: 50, Cell Signaling Technologies [CST], Beverly, MA) or IgG (#3423, 1: 20, CST) overnight at 4 °C. Thereafter, the sample was added with 20 μL protein A/G-sepharose microspheres

(16) The role of the atypical chemokine receptor CCRL2 in myelodysplastic syndrome and secondary acute myeloid leukemiaScience advancesFebruary 18, 2022Karantanos T, Teodorescu P, Perkins B, Christodoulou I, Esteb C, Varadhan R, Helmenstine E, Rajkhowa T, Paun BC, Bonifant C, et al.(Cell Signaling Technology, #4089) or Sepharose bead conjugate isotype control (Cell Signaling Technology, #3423). Beads were then washed extensively and boiled with 30 μl of loading buffer. Colony formation assay Clonogenic

(17) MCTR1 Intervention Reverses Experimental Lung Fibrosis in MiceJournal of Inflammation ResearchMay 11, 2021Jingyi Pan, Xinyu Li, Xinyang Wang, Lili Yang, Houlin Chen, Nana Su, Chenghua Wu, Yu Hao, Shengwei Jin, Hui Limortality in Age Gene Environment Susceptibility-Reykjavik (AGES-Reykjavik) Study. Eur Heart J. 2017;38(46):3423–3430. doi:10.1093/eurheartj/ehx510 19. Oh K, Park HB, Byoun OJ, et al. Epithelial transglutaminase 2

(18) OTUD1 Activates Caspase-Independent and Caspase-Dependent Apoptosis by Promoting AIF Nuclear Translocation and MCL1 DegradationAdvanced science (Weinheim, Baden-Wurttemberg, Germany)April 1, 2021Luo Q, Wu X, Zhao P, Nan Y, Chang W, Zhu X, Su D, Liu Z.Cell Signaling Technology, #5939); anti‐AIF, 1:50 (PLA; Abcam, ab110327); anti‐IgG, 1:20 (IP; Cell Signaling Technology, #3423); anti‐V5‐Tag, 1:1000 (IB; Cell Signaling Technology, #13 202); anti‐Flag‐Tag, 1:1000 (IB; Cell Signaling Technology

(19) Mitophagy-mediated adipose inflammation contributes to type 2 diabetes with hepatic insulin resistanceThe Journal of experimental medicineMarch 1, 2021He F, Huang Y, Song Z, Zhou HJ, Zhang H, Perry RJ, Shulman GI, Min W.cell lysates were precleared for 1 h with normal rabbit IgG (Cell Signaling Technology; catalog no. 3423) and protein A/G agarose beads (Santa Cruz; catalog no. sc-2003) that had been prewashed with RIPA buffer.

(20) Catecholamines suppress fatty acid re-esterification and increase oxidation in white adipocytes via STAT3Nature metabolismJuly 1, 2020Reilly SM, Hung CW, Ahmadian M, Zhao P, Keinan O, Gomez AV, DeLuca JH, Dadpey B, Lu D, Zaid J, et al.added for 2 h, or lysates were incubated with STAT3 (Cell Signaling – 4368) or IgG (Cell Signaling − 3423) antibody coated beads overnight. Flow through was collected and the beads were washed 5 times with

Be the First to Write a Review!

Rabbit (DA1E) mAb IgG XP ® Isotype Control (Sepharose ® Bead Conjugate)

Rabbit (DA1E) mAb IgG XP ® Isotype Control (Sepharose ® Bead Conjugate) from Cell Signaling Technology

Description

Cell Signaling Technology

Citations (31)

Reviews

Rabbit (DA1E) mAb IgG XP ® Isotype Control (Sepharose ® Bead Conjugate) from Cell Signaling Technology

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map