RPA32/RPA2 (4E4) Rat mAb 2208S from Cell Signaling Technology

Product Specs
ItemRPA32/RPA2 (4E4) Rat mAb
CompanyCell Signaling Technology
Price $336.00 Inquire
Catalog Number2208S
Quantity100 µl
ApplicationsW, IP, IF-IC, FC-FP
ReactivityH, M, R, Hm, Mk
TargetRPA32
HostRat
NCBI Gene AliasesREPA2, RP-A p32, RP-A p34, RPA32
ClonalityMonoclonal
NCBI Full Gene Namereplication protein A2
Research AreaCell Cycle / Checkpoint Control
IsotypeIgG1
Clone40000
Molecular Weight32

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Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) RPA hyperphosphorylation hinders the resolution of R-loops and G-quadruplex-associated R-loops during RAS-driven senescenceNucleic Acids ResApril 13, 2026Ylenia Cortolezzis, Vanessa Tolotto, Luca Triboli, Raffaella Picco, Miguel A Soler, Sara Fortuna, Giacomo Bettin, Francesca D'Este, Enrico Carlassara, Gabriele Magris, et al.Signaling Technology), 3 μg of IgG (Active Motif), 4μg of RNA Pol II Ser5 (1H4B6 Merck), 3 μg of RPA32 (2208 Cell Signaling Technology), 3 μg of phosphoRPA32 (A300-245A Bethyl), 3 μg of RNase H1 (Merck), and γH2AX

(2) CHK1 is an integral regulator of DNA replication in human cellsCell Death & DiseaseMarch 26, 2026Siting Li, Dandan Zhu, Mengfan Tang, Min Huang, Xu Feng, Litong Nie, Huimin Zhang, Ling Yin, Sarah Keast, Chang Yang, et al.(Bethyl Laboratories, A300-245A); phospho-RPA2 (Ser33) (Bethyl Laboratories, A300-246A); RPA2 (CST, #2208S); phospho-CHK1 (Ser296) (CST, #90178S); phospho-CHK1 (Ser317) (CST, #12302S); phospho-CHK1 (Ser345)

(3) Primordial germ cells experience increasing physical confinement and DNA damage during migration in the mouse embryoSci AdvMarch 20, 2026Katharine Goodwin, Theresa Anne Emrich, Sebastian Arnold, Katie McDole(rabbit, 1:200, Sigma-Aldrich, L9393), MAB414 (mouse, 1:200, Abcam, ab24609), RPA32 (rat, 1:100, CST, 2208), Sox2 (rabbit, 1;200, Abcam, ab97959), SSEA1 (mouse, 1:200, Novus Biologicals, MC-480), Stella (goat,

(4) Tex11 Mutant Mouse Models of Human AzoospermiabioRxivFebruary 23, 2026Georgia Rae Atkins, Rachel L. Hvasta-Gloria, Chatchanan Ausavarungnirun, Christopher R. Pombar, Jimmaline J. Hardy, Meena Sukhwani, Emily P. Barnard, Nijole Pollock, Michelle Malizio, Yi Sheng, et al.antibodies included: anti-mouse SYCP3 (ab97672), SYCP1 (ab15090), yH2AX (ab26350), RPA (Cell Signaling #2208S), RAD51 (Millipore #PC130–100U) and MLH1 (BD Biosciences Pharmingen, #550838). Alexa Fluor secondary

(5) RPA-independent activation of the ATR/CHK1 pathwayProceedings of the National Academy of SciencesFebruary 10, 2026Min Huang, Dandan Zhu, Junjie ChenAntibodies against RPA2 purchased from Abcam (cat. no. 2175) and Cell Signaling Technology (cat. no. 2208) were respectively used in HEK293A and RPE-1 cells. An antibody against vinculin (cat. no. V9264) was

(6) CD40 drives tumor growth in non-small cell lung cancer via non-canonical immune-independent mechanismsCancer LettersJanuary 16, 2026Yujie Liu, Chandra Bhushan Prasad, Zhaojun Qiu, Deepika Singh, Nicholas Bean, Shravya Honne, Xiaoli Zhang, Chunhong Yan, Zaibo Li, Baek Kim, et al.dilution): CD40 (#40468), CD40L (#15094), p-CHK1 (S345, #12302), CHK1 (#2360), p-p53 (S20) (#9287), RPA32 (#2208), cleaved PARP1 (#5625), p-NF-kB (#3033), NF-kB (#6956), c-Jun (#9165), p38 MAPK (8690), p-p38 MAPK

(7) RPA exhaustion activates SLFN11 to eliminate cells with heightened replication stressNature Cell BiologyJanuary 9, 2026Tyler H. Stanage, Shudong Li, Sandra Segura-Bayona, Aurora I. Idilli, Rhona Millar, Graeme Hewitt, Simon J. Boultontargeting BrdU (mouse, BD Biosciences, catalogue number 555627, 1:250), RPA32 (rat, CST, catalogue number 2208 1:500) and either ATF4 (rabbit, CST, catalogue number 11815, 1:500) or γH2AX–pSer139 (rabbit, CST, catalogue

(8) Chromatin assembly by the histone chaperone HIRA facilitates Human Papillomavirus replicationTumour Virus ResearchDecember 1, 2025Ashley N. Della Fera, Dan Chen, Alison A. McBrideDaxx rabbit polyclonal (Sigma, HPA008736; 1:100), RPA32/RPA2 (4E4) rat monoclonal (Cell Signaling, 2208; 1:300), PML (H-238) rabbit polyclonal (Santa Cruz, sc-5621; 1:100), Sp100 rabbit polyclonal (Sigma,

(9) Dihydroxyacetone decreases the dATP pool, inducing replication stress and genomic instability in BEAS-2B cellsJournal of Biological ChemistryDecember 1, 2025Saddam Hussain, Nayonika Mukherjee, Natalie R. Gassman(#93994),1:1000,Cell Signaling Technology pRPA32-S33 (#10148),1:1000,Cell Signaling Technology RPA32 (#2208),1:3000,Cell Signaling Technology RPA32 (#ab2175) (IF),1:1000,Abcam 53BP1 (NBP100-304),1:500,Novus

(10) Design and Development of DNA Damage Chemical Inducers of Proximity (DD-CIP) for Targeted Cancer TherapybioRxivNovember 6, 2025Tian Qiu, Yeuan Ting Lee, Brendan G. Dwyer, Yi Jer Tan, Ting Chen, Bryan A. Romero, Yanlan Wang, Jiehui Deng, Tinghu Zhang, Gerald R. Crabtree, et al.Cell Signaling Technology, Danvers, MA, USA), anti-FLAG M2 (cat. no. F1804; Sigma-Aldrich), anti-RPA32/RPA2 (2208, Cell Signaling Technology, Danvers, MA, USA), anti-phospho-RPA32 S4/S8 (A700-009-T, Bethyl Laboratories,

(11) Lnk Deficiency Enhances Translesion Synthesis to Alleviate Replication Stress and Promote Hematopoietic Stem Cell FitnessJournal of Clinical InvestigationOctober 30, 2025Brijendra Singh, Md Akram Hossain, Xiao Hua Liang, Jeremie Fages, Carlo Salas Salinas, Roger A. Greenberg, Wei TongCell Signaling Technology 3638S), anti-PCNA (1:1,000; Cell Signaling Technology 13110S), anti-RPA2 (1:1,000; Cell Signaling 2208), anti–pSer4/8 RPA2 (1:1,000; Bethyl A300-245A), anti–pSer33 RPA2 (1:1,000; Bethyl A300-246A), anti–pSer108

(12) Phase separation of ERCC6L2–CtIP regulates the extent of DNA end resectionNature Cell BiologySeptember 5, 2025Yixin Yin, Jinlong Lin, Xiaoxia Cai, Runcong Nie, Jiliang Lin, Shuting Li, Zhicheng Xiang, Yihong Ling, Yiyang Zhang, Jie Zhou, et al.ab133534, used for IF at 1:200, and IB at 1:1,000 or Proteintech 67024, used for IF at 1:100), RPA2 (CST 2208S, used for IB at 1:1,000, IF at 1:200), BRCA1 (CST 9010S, used for IB at 1:1,000; Santa Cruz sc-6954,

(13) The full length BEND2 protein is dispensable for spermatogenesis but required for setting the ovarian reserve in miceElifeAugust 20, 2025Yan Huang, Nina Bucevic, Carmen Coves, Natalia Felipe-Medina, Marina Marcet-Ortega, Nikoleta Nikou, Cristina Madrid-Sandín, Maria López-Panadés, Carolina Buza, Neus Ferrer Ferrer Miralles, et al.(1:400) Antibody,anti-MLH1 Ms,BD Biosciences,51-1327GR,IF (1:50) Antibody,RPA32 (4E4) Rat,Cell Signalling,2208 S,IF (1:100) Antibody,anti-RAD51 (ab-1) Rb,Millipore,PC130,IF (1:100) Antibody,anti-GFP Rb,Thermo Fisher Scientific

(14) Conserved and unique features of terminal telomeric sequences in ALT-positive cancer cellseLifeAugust 1, 2025Benura Azeroglu, Wei Wu, Raphael Pavani, Ranjodh Singh Sandhu, Tadahiko Matsumoto, André Nussenzweig, Eros Lazzerini-DenchiF1006). Primary antibodies used in this study were TRF2 (NB110-57130, Novus Biologicals, rabbit), RPA32/RPA2 (2208, Cell Signaling, rat), Myc (2276, Cell Signaling, mouse), and 53BP1 (NB100-304, Novus Biologicals, rabbit). Imaging

(15) UPR-induced intracellular C5aR1 promotes adaptation to the hypoxic tumour microenvironmentCell Death DisJuly 22, 2025Tatsuya Suwa, Kelly SW. Lee, Ian J. Chai, Heather O. L. Clark, David J. MacLean, Nicole Machado, Gonzalo Rodriguez-Berriguete, Lolita Singh, Geoff S. Higgins, Ester M. Hammond, et al.primary antibodies were used; γ-H2AX (Ser139) (Sigma-Aldrich, 05-636), RPA32/RPA2 (Cell Signalling, 2208), CD88 (BD Biosciences, 550493), LAMP1 (Cell Signalling, 9091), AIF (Cell Signalling; 5318), PDI (Cell Signalling

(16) USP37 counteracts HLTF to protect damaged replication forks and promote survival of BRCA1-deficient cells and PARP inhibitor resistanceNucleic Acids ResJune 23, 2025Mengfan Tang, Siting Li, Ziqiang Zhu, Chao Wang, Shuai Ding, Huimin Zhang, Min Huang, Sarah Keast, Zhen Chen, Ling Yin, et al.Millipore Sigma), Flag (F3165, Millipore Sigma), β-tubulin (T5168, Millipore Sigma), RAD51 (ab63801, Abcam), RPA2 (2208S, Cell Signaling Technology), USP37 (ab190184, Abcam), HLTF (43345S, Cell Signaling Technology), 53BP1

(17) Conserved and Unique Features of Terminal Telomeric Sequences in ALT-Positive Cancer CellsbioRxivJune 2, 2025Benura Azeroglu, Wei Wu, Raphael Pavani, Ranjodh Sandhu, Tadahiko Matsumoto, André Nussenzweig, Eros Lazzerini Lazzerini DenchiF1006). Primary antibodies used in this study were TRF2 (NB110–57130, Novus Biologicals, rabbit), RPA32/RPA2 (2208, Cell Signaling, rat), Myc (2276, Cell Signaling, mouse) and 53BP1 (NB100–304, Novus Biologicals, rabbit). Imaging

(18) Histone chaperones coupled to DNA replication and transcription control divergent chromatin elements to maintain cell fateGenes & DevelopmentMay 2, 2025Reuben Franklin, Brian Zhang, Jonah Frazier, Meijuan Chen, Brian T. Do, Sally Padayao, Kun Wu, Matthew G. Vander Heiden, Christopher R. Vakoc, Jae-Seok Roe, et al.2360S), anti-pRPA32-S33 (Cell Signaling Technologies 10148), anti-RPA32 (Cell Signaling Technologies 2208S), anti-TBP (Abcam ab818), anti-β-Actin (Millipore Sigma A3854), antimouse IgG (Millipore Sigma AP124P),

(19) Small molecule APOL1 inhibitors as a precision medicine approach for APOL1-mediated kidney diseaseNature communicationsJanuary 2, 2025Zimmerman B, Dakin LA, Fortier A, Nanou E, Blasio A, Mann J, Miller H, Fletcher M, Wang T, Nanthakumar S, et al.(Chromomap DAB Kit; Cat #760-159) for 8 min. Slides were counterstained for 8 min (Hematoxylin II, Cat # 970-2208), followed by bluing for 4 min (Bluing reagent, Cat # 760-2037) and coverslipped. Rabbit monoclonal

(20) Transcription and DNA replication collisions lead to large tandem duplications and expose targetable therapeutic vulnerabilities in cancerNature CancerNovember 18, 2024Yang Yang, Michelle L. Badura, Patrick C. O’Leary, Henry M. Delavan, Troy M. Robinson, Emily A. Egusa, Xiaoming Zhong, Jason T. Swinderman, Haolong Li, Meng Zhang, et al.1:1,000), vinculin (Cell Signaling, cat. no. 13901, 1:1,000), RPA32 total (Cell Signaling, cat. no. 2208, 1:1,000), RPA32 pS33 (Cell Signaling, cat. no. 10148, 1:1,000), RPA32 pS4/8 (Bethyl, cat. no. A300-245A-M,

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RPA32/RPA2 (4E4) Rat mAb

RPA32/RPA2 (4E4) Rat mAb from Cell Signaling Technology

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Cell Signaling Technology

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RPA32/RPA2 (4E4) Rat mAb from Cell Signaling Technology

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