beta-Tubulin (9F3) Rabbit mAb (Alexa Fluor ® 488 Conjugate) 3623S from Cell Signaling Technology

Product Specs
Itembeta-Tubulin (9F3) Rabbit mAb (Alexa Fluor ® 488 Conjugate)
CompanyCell Signaling Technology
Price $450.00 Inquire
Catalog Number3623S
Quantity100 µl
ApplicationsIF-IC, FC-FP
ReactivityH, M, R, Mk, Z, B
Targettubulin beta-5
HostRabbit
NCBI Gene AliasesCDCBM6, CSCSC1, M40, OK/SW-cl.56, TUBB1, TUBB5
Conjugate/TagAlexa Fluor ® 488
ClonalityMonoclonal
NCBI Full Gene Nametubulin beta class I
Research AreaCytoskeletal Signaling
IsotypeIgG
Clone9F3

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Extracellular signalling regulates gastrin transcription through site-specific phosphorylation and nuclear redistribution of MeninbioRxivApril 13, 2026Uloma B. Elvis-Offiah, Ziyu Wen, Xianxin Hua, Juanita L. MerchantCell Signaling, #8878) or Alexa Fluor® 488-conjugated anti-β-Tubulin (clone 9F3) (1:200; Cell Signaling, #3623) to visualize the cytoplasmic compartment. Following washes in TBS, cells were mounted using ProLong

(2) A sphingolipid-derived paclitaxel nanovesicle enhances efficacy of combination therapies in triple-negative breast cancer and pancreatic cancerNature cancerOctober 1, 2025Wang Z, Li W, Jiang Y, Ma T, Li M, Wu S, Tran TB, Cordova LE, Lin E, Scott AJ, et al.sections were immunostained with β-tubulin Alexa Fluor® 488-conjugated antibody (1:100, Cell Signaling #3623S) and DAPI, then imaged using an Olympus VS200 slide scanner and processed with OlyVIA software (version

(3) Therapeutic Application of Dendrobium fimbriatum Hook for Retinopathy Caused by Ultraviolet Radiation and Chemotherapy Using ARPE-19 Cells and Mouse RetinaPlants (Basel)February 23, 2024Chi-Feng Cheng, Sheue-Er Wang, Chen-Wen Lu, Thi Kim Ngan Nguyen, Szu-Chuan Shen, Chia-Ying Lien, Wu-Chang Chuang, Ming-Chung Lee, Chung-Hsin Wu(TNF)-α (Cat. numbers #3707; Cell Signaling Technology, Danvers, MA, USA), and caspase 3 (Cat. numbers #3623; Cell Signaling Technology) for 1 h at room temperature, respectively. Sections were incubated with

(4) Initial spindle positioning at the oocyte center protects against incorrect kinetochore-microtubule attachment and aneuploidy in miceScience AdvancesFebruary 17, 2023Jessica N. Kincade, Avery Hlavacek, Takashi Akera, Ahmed Z. Balboulaanti–β-tubulin (9F3) monoclonal conjugated to Alexa Fluor 488 (1:50; Cell Signaling Technology, no. 3623), N-WASP (1:100 Cell Signaling Technology, no. 30D10), anti-human Alexa Fluor 647 (1:200; Cell Signaling

(5) Exosomal proteomic signatures correlate with drug resistance and carboplatin treatment outcome in a spontaneous model of canine osteosarcomaCancer Cell InternationalDecember 1, 2021Marcus A. Weinman, Stephen A. Ramsey, Haley J. Leeper, Jacqueline V. Brady, Andrew Schlueter, Stanislau Stanisheuski, Claudia S. Maier, Tasha Miller, Carl E. Ruby, Shay Brachaassociates with poor prognosis of patients with gastric cancer after gastrectomy. J Cancer. 2017;8(17):3623–30. 42. Holtet TL, et al. Tetranectin, a trimeric plasminogen-binding C-type lectin. Protein Sci. 1997;6(7):1511–5. 43.

(6) Hfm1 participates in Golgi-associated spindle assembly and division in mouse oocyte meiosisCell death & diseaseJune 30, 2020Wang H, Zhong C, Yang R, Yin Y, Tan R, Gao L, Gao C, Cui Y, Pu D, Wu J.antibody (610822, 1:100, BD Biosciences), a Alexa Fluor 488 conjugate β-Tubulin (9F3) Rabbit antibody (3623, 1:200, CST) or a Rabbit anti-Phospho-p44/42 MAPK (Erk1/2) antibody (4370, 1:100, CST) overnight at

(7) Genome-wide analysis reveals a switch in the translational program upon oocyte meiotic resumptionNucleic acids researchApril 6, 2020Luong XG, Daldello EM, Rajkovic G, Yang CR, Conti M.blocking buffer. The antibodies used were: 1:100 β-tubulin (9F3) rabbit mAb (Cell Signaling Technology, 3623); 1:200 human antibody against centromere (ImmunoVision, HCT-0100). After another round of washing,

(8) Extraordinary Transmission and Radiation From Finite by Infinite Arrays of SlotsIEEE Transactions on Antennas and PropagationJanuary 1, 2020Miguel Camacho, Rafael R. Boix, Francisco Medina, Alastair P. Hibbins, J. Roy Samblespolarization multilayered reflectarray antennas made of dipoles,” IEEE Trans. Antennas Propag., vol. 65, pp. 3623-3637, no. 7, July 2017. [16] R. E. Collin, Antennas and Radiowave propagation, New York: McGraw-Hill,

(9) CETSA-based target engagement of taxanes as biomarkers for efficacy and resistanceScientific reportsDecember 18, 2019Langebäck A, Bacanu S, Laursen H, Mout L, Seki T, Erkens-Schulze S, Ramos AD, Berggren A, Cao Y, Hartman J, et al.Cell Signalling was used since that is the same clone (9F3) as the Alexa Fluor 488 conjugated antibody #3623 that was used for Image-CETSA. Secondary antibodies used were either sc-2055 from Santa Cruz or W402B

(10) Cyclin B2 is required for progression through meiosis in mouse oocytesDevelopment (Cambridge, England)April 26, 2019Daldello EM, Luong XG, Yang CR, Kuhn J, Conti M.blocking buffer. The antibodies used were as follows: β-tubulin-488, 1:100 (Cell Signaling Technology, 3623); CREST, 1:200 (ImmunoVision, HCT-0100); and MAD2, 1:200 (Dr Rey-Huei Chen, Academia Sinica, Taipei).

(11) CENP-A Modifications on Ser68 and Lys124 Are Dispensable for Establishment, Maintenance, and Long-Term Function of Human CentromeresDevelopmental CellJanuary 1, 2017Daniele Fachinetti, Glennis A. Logsdon, Amira Abdullah, Evan B. Selzer, Don W. Cleveland, Ben E. Blackat 1 μg/mL. β-tubulin rabbit monoclonal antibody conjugated to AlexaFluor488 (9F3; Cell Signaling; #3623) was used at 1:100 dilution. FITC- and Cy5-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories,

(12) Time-resolved multimodal analysis of Src Homology 2 (SH2) domain binding in signaling by receptor tyrosine kinaseseLifeApril 12, 2016Jadwin JA, Oh D, Curran TG, Ogiue-Ikeda M, Jia L, White FM, Machida K, Yu J, Mayer BJ.Models for Binding, Trafficking, and Signaling. Oxford: Oxford University Press. doi: 10.1016/s0306-3623(97)90087-2 Lemmon MA, Schlessinger J. 2010. Cell signaling by receptor tyrosine kinases. Cell 141:1117–1134.

(13) Signal transduction in primary human T lymphocytes in altered gravity – results of the MASER-12 suborbital space flight missionCell Communication and SignalingDecember 1, 2013Svantje Tauber et al.CD3 (PromoCell, PK-AB913144), 1:80; IL-2R (PromoCell,PK-AB913-104), 1:40; βTubulin (Cell Signaling, 3623), 1:640; Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) (Cell Signaling, 4344), 1:160; Phospho-p44/42

(14) Graphene microsheets enter cells through spontaneous membrane penetration at edge asperities and corner sitesProceedings of the National Academy of SciencesJuly 23, 2013Yinfeng Li, Hongyan Yuan, Annette von dem Bussche, Megan Creighton, Robert H. Hurt, Agnes B. Kane, Huajian Gaostained with antitubulin antibody conjugated with ﬂuorescein isothiocyanate (FITC) (Cell Signaling; 3623S) and 4′,6-diamidino-2-phenylindole (DAPI). Macrophages were exposed to graphene for 5 h as described

(15) Maternally recruited Aurora C kinase is more stable than Aurora B to support mouse oocyte maturation and early developmentProceedings of the National Academy of SciencesAugust 14, 2012Karen Schindler, Olga Davydenko, Brianna Fram, Michael A. Lampson, Richard M. Schultzepitope BL1217) at 1:30 or a polyclonal anti–β-tubulin antibody conjugated to Alexa 488 (Cell Signaling 3623) at 1:75 in blocking buffer for 1 h at room temperature in a humidiﬁed chamber. After washing, secondary

(16) Maternally-Recruited Aurora C Kinase Is More Stable Than Aurora B to Support Mouse Oocyte Maturation and Early DevelopmentBiology of ReproductionAugust 1, 2012Karen Schindler, Olga Davydenko, Brianna Fram, Michael A. Lampson, Richard Mepitope BL1217) at 1:30 or a polyclonal anti–β-tubulin antibody conjugated to Alexa 488 (Cell Signaling 3623) at 1:75 in blocking buffer for 1 h at room temperature in a humidiﬁed chamber. After washing, secondary

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beta-Tubulin (9F3) Rabbit mAb (Alexa Fluor ® 488 Conjugate)

beta-Tubulin (9F3) Rabbit mAb (Alexa Fluor ® 488 Conjugate) from Cell Signaling Technology

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Cell Signaling Technology

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beta-Tubulin (9F3) Rabbit mAb (Alexa Fluor ® 488 Conjugate) from Cell Signaling Technology

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