Mcl-1 (D35A5) Rabbit mAb 5453S from Cell Signaling Technology

Product Specs
ItemMcl-1 (D35A5) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number5453S
Quantity100 µl
ApplicationsW, W-S
ReactivityH, M, Mk
TargetMCL1
HostRabbit
NCBI Gene AliasesBCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, Mcl-1, TM, bcl2-L-3, mcl1/EAT
ClonalityMonoclonal
NCBI Full Gene NameMCL1 apoptosis regulator, BCL2 family member
Research AreaApoptosis
IsotypeIgG
CloneD35A5
Molecular Weight40 (human), 35 (mouse)

Questions and Answers

Can this antibody be used to study Mcl-1 in various cancer models?

Yes, the Mcl-1 (D35A5) Rabbit mAb has been utilized in multiple studies to investigate the role of Mcl-1 in cancer. For instance, it was employed in Western blot analyses to assess Mcl-1 expression in various cancer cell lines, including those resistant to apoptosis, highlighting its significance in survival signaling pathways in malignancies such as acute myeloid leukemia and small-cell lung cancer.

Is this antibody effective for detecting Mcl-1 in different species?

The Mcl-1 (D35A5) Rabbit mAb is reactive with human, mouse, and non-human primate samples, making it suitable for comparative studies across these species. This broad reactivity allows researchers to explore Mcl-1's role in apoptosis regulation in various biological contexts.

How has this antibody been applied in research related to apoptosis?

This antibody has been shown to be effective in studies examining apoptosis mechanisms. For example, it was used to analyze the expression levels of Mcl-1 in response to treatments that induce apoptosis, such as the combination of azacytidine and erlotinib, which was found to enhance apoptotic signaling through the downregulation of anti-apoptotic proteins.

Are there any specific experimental conditions or treatments where this antibody has been particularly useful?

The Mcl-1 (D35A5) Rabbit mAb has been particularly useful in experiments involving Western blotting to evaluate the effects of various treatments on Mcl-1 levels. For instance, it was cited in studies assessing the synergistic effects of drugs like ABT-737 and fenretinide on neuroblastoma cells, where changes in Mcl-1 expression were critical for understanding the apoptotic response.

Cell Signaling Technology

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Phone: 978-867-2388
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Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Therapeutic targeting of BCL-2 during CART cell production augments potency through non-apoptotic adaptive changesSignal Transduct Target TherApril 27, 2026Nada S. Aboelella, Ryan Park, Erting Tang, Nicholas Asby, Joshua D. Ho, Tony Pan, Sidney Wang, Lishi Xie, Justin P. Kline, Peter A. Riedell, et al.#4223), BCL-XL (1:1000; Cell Signaling Technology, #2764), MCL-1 (1:1000; Cell Signaling Technology, #5453), and β-Actin (1:3000; Cell Signaling Technology, #3700). Primary antibodies were washed out with 1×

(2) Genome-wide profiling identifies the genetic dependencies of cell death following EGFR inhibitionJ Biol ChemApril 2, 2026Sydney A. Porto, Gavin A. Birdsall, Nicholas W. Harper, Megan E. Honeywell, Scott M. Leighow, Tiana E. Naylor, Kelly M. Ward, Mika K. Wesley, Justin R. Pritchard, Michael J. Lee3′ Immunoblotting Immunoblotting was performed using the LiCOR Odyssey CLx infrared scanner. PTEN (9188T), BIM (2933), Mcl-1 (5453S), and MYC (5605S) rabbit monoclonal antibodies were purchased from Cell Signaling Technologies. β-actin

(3) Role of lipid rafts in the FGFR2c-mediated oncogenic signaling by involvement of TRPA1 channel in pancreatic ductal adenocarcinoma cellsCell Death & DiseaseFebruary 24, 2026Vanessa Mancini, Valeria Manganelli, Tina Garofalo, Maurizio Sorice, Salvatore Raffa, Dafne Scullari, Danilo Ranieri, Francesca BelleudiCambridge, UK; ab63387), anti-Myeloid Cell Leukemia 1 (Mcl-1) (D35A5) (Cell Signaling; Beverly, MA, USA; 5453), anti-TRPA1 (Abcam; Abcam Cambridge, UK; ab62053),. The membranes were stripped as reported [6] and

(4) P300 enhances glycolysis and dox resistance in DLBCL by upregulating HK2 expression through histone lactylationBMC CancerJanuary 31, 2026Xiangxiang Song, Huazhen Fu, Xing Zhong, Nasha Yu, Weiming ZhangName,Brand,Number,Dilution rate,Applications β-actin,CST,#4970,1:5000,WB P300,affinity,AF5360,1:1000,"WB, IHC" Mcl-1,CST,#5453,1:1000,"WB, IHC" Bcl-2,CST,"#15,071",1:1000,WB Bcl-XL,CST,#2764,1:1000,WB Bax,abcam,ab32503,1:1000,WB Bak,CST,#6947,1:2000,WB HK2,CST,#2867,1:2000,"WB,

(5) Disrupting CDK9 activity suppresses triple-negative breast cancer and is enhanced by EGFR InhibitionCell Oncol (Dordr)January 8, 2026Vera E. van der Noord, Ronan P. McLaughlin, Jessica S. Karuntu, Jichao He, A. Mieke Timmermans, Sunita K. C. Basnet, Yi Long, Sarah Al Haj Diab, Solomon Tadesse, Natalie Proost, et al.#13523), RNAPII (CST #2629), Survivin (CST #2808), BCL-xL (CST #2764), XIAP (CST #2045), MCL1 (CST #5453), γ-H2AX (S139; CST #2577) and tubulin (Sigma Aldrich T-9026). Cell culture and compound screening Authenticated

(6) Tegavivint triggers TECR-dependent non-apoptotic cancer cell deathNature Chemical BiologyDecember 1, 2025Logan Leak, Ziwei Wang, Alby J. Joseph, Brianna Johnson, Alyssa A. Chan, Cassandra M. Decosto, Leslie Magtanong, Pin-Joe Ko, Weaverly Colleen Lee, Joan Ritho, et al.caspase-3 (1:1000, Cat# 9664T, Cell Signaling Technologies, Danvers, MA), rabbit anti-MCL1 (1:1000, Cat# 5453T, Cell Signaling Technologies), mouse anti-tubulin (1:2000, Cat# MS581P1, Fisher Scientific, Hampton,

(7) Discovery of a “First-in-Class” Selective Multikinase (CDK4/6/9-AURKA/B) Inhibitor, LCI133, for NeuroblastomaJournal of Medicinal ChemistryNovember 27, 2025Krishnaiah Maddeboina, Dhananjaya Pal, Bharath Yada, Cody C. McHale, Sandeep K. Singh, Yashpal S. Chhonker, Hailey L. Dryden, Ritchie Delara, Vaidehi Mujumdar, Qi Zhang, et al.following antibodies: pRpb1 (CST# 13499S), pRpb1 Ser5 (CST# 13523S), Total Rpb1 (CST# 2629S), MCL-1 (CST# 5453S), and N-Myc (Sc-53993). Secondary antibodies were chosen according to the species of origin of the primary

(8) Chlamydia trachomatis subverts neutrophil cell death pathways through RIP3 and Mcl-1 manipulationmBioNovember 12, 2025Rebecca Koch, Naveen Challagundla, Kathrin Stelzner, Thomas Rudelwere purchased from Cell Signaling Technology and include pAkt (Cat#4060), Akt (Cat#9272), Mcl-1 (Cat#5453), pIĸBα (Cat#2859), IĸBα (Cat#4812), pRIP3 (Cat#93654), and RIP3 (Cat#13526). GAPDH was obtained from

(9) CDK12 regulates cellular metabolism to promote glioblastoma growthJCI InsightNovember 10, 2025Jeong-Yeon Mun, Chang Shu, Qiuqiang Gao, Zhe Zhu, Hasan O. Akman, Mike-Andrew Westhoff, Georg Karpel-Massler, Markus D. Siegelinagainst the following proteins were used: CDK12 (Cell Signaling Technology [CST], 11973), Mcl-1 (CST, 5453), Bcl-2 (clone D55G8, CST, 4223), Bcl-xL (clone 54H6, CST, 2764), Noxa (clone 114C307, Calbiochem, OP180),

(10) The anticancer effect of the HDAC inhibitor belinostat is enhanced by inhibitors of Bcl‐ x L or Mcl‐1 in ovarian cancerMolecular OncologyNovember 1, 2025Cécilia Thomine, Sterenn Guillemot, Louis‐Bastien Weiswald, Romane Florent, Edwige Abeilard, Florence Giffard, Emilie Brotin, Mélanie Briand, Enora Dolivet, Laurent Poulain, et al.(Lys9) (#9649), Caspase‐3 (#9662), PARP (#9542), Bim (#2819), Puma (#12450), Bcl‐xL (#2764) and Mcl‐1 (#5453) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA), Noxa antibody (#OP180)

(11) Redox destabilization by ibrutinib promotes ferroptosis in diffuse large B-cell lymphoma (DLBCL)Cell Death DiscoveryOctober 31, 2025Anuschka Langpape, Debora Bonasera, Jenny Stroh, Moritz Reese, Maria Cartolano, Gianmaria Liccardi, Silvia von KarstedtPA5-27150), TRXR1 (CST, 15140S), p-P70 (CST, #9206), cFLIP (CST, 56343), c-MYC (CST, 5605), MCL-1 (CST, 5453), xCT (abcam, ab37185), β-Actin (Sigma, A1978). For secondary antibodies α-rabbit IgG (211-032-171)

(12) Structure-guided design of a methyltransferase-like 3 (METTL3) proteolysis targeting chimera (PROTAC) incorporating an indole–nicotinamide chemotype†RSC Medicinal ChemistrySeptember 17, 2025Annabelle C. Weldert, Ariane F. Frey, Mackenzie W. Krone, Franziska Krähe, Hannah Kuhn, Christian Kersten, Fabian Barthelsab220030), anti-Bcl2 (1 : 1000, Cell Signaling Technology [CST], EPR16891), anti-Mcl-1 (1 : 1000, CST, 5453 T), anti-Bcl-XL (1 : 1000, CST, 2764 T), anti-vinculin (1 : 1000, CST, 13901) or anti-GAPDH (1 : 5000,

(13) Breaking the cancer code: a novel DNA minicircle to disable STAT3 in ovarian cancer cells SKOV3Frontiers in PharmacologySeptember 15, 2025Adina-Gabriela Vasilescu, Andrei-Mihai Vasilescu, Livia Elena Sima, Natalia Baran, Ștefan-Eugen Szedlacsekrabbit monoclonal (Cell Signaling, Cat no 9661S), anti-Mcl-1, rabbit monoclonal (Cell Signaling, Cat no 5453T), anti-alpha Tubulin, rabbit polyclonal (Abcam, Cat no ab15246) and anti-Pim-1, rabbit monoclonal (Cell Signaling

(14) Rupatadine suppresses tumor growth and EMT in pancreatic cancer: Evidence from in vitro and in vivo modelsMolecular Medicine ReportsSeptember 5, 2025Byung Jo Choi, Ho Joong Choi, Dosang Lee, Jung Hyun Park, Tae Ho Hong, Hee-Jung Jung, Ga-Hee Jo, Ok-Hee Kim, Say-June Kimantibodies used targeted hPARP (Cell Signaling Technology, #9542), MCL-1 (Cell Signaling Technology, #5453), cleaved-caspase-3 (Cell Signaling Technology, #9664), TGF-βR1 (Abcam, ab31013), p-SMAD2/3 (Cell Signaling Technology

(15) MCL1 may not mediate chemoresistancebioRxivSeptember 1, 2025Kylin A. Emhoff, Kunho Chung, Dongmei Zhang, Belinda Willard, Timothy Chan, Babal Kant Jha, Shaun R. Stauffer, Jesse A. Coker, Jan Joseph MelenhorstTBST for 1 h at room temperature. Blots were then incubated with MCL1 (1:1000 dilution, anti-rabbit; #5453, Cell Signaling Technology), FLAG (1:1000 dilution, anti-rabbit; #14793S, Cell Signaling Technology),

(16) Survivin Is a Central Mediator of Cell Proliferation in HPV-Negative Head and Neck Squamous Cell CarcinomaCancersAugust 31, 2025Zhu J, An J, Hu E, Rosenblatt G, Berner G, Roy A, Kawachi N, Shrivastava N, Mehta V, Segall JE, et al.β-actin (no. 3700), rabbit mAb against Bax (no. 2772), Bcl-xL (no. 2764), Bim (no. 2819), Mcl-1 (no. 5453), and survivin (no. 2808) were from Cell Signaling Technology (Danvers, MA, USA). Rabbit polyclonal

(17) Inhibition of NAMPT as a therapeutic strategy to suppress tumor growth in lymphangioleiomyomatosisBiochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAugust 1, 2025Shahrzad S. Fard, Nandini Kundu, Alek S. Torres, Christina L. Faltas, Julie S. Di Martino, Marina K. Holz(2280S), p-raptor S792 (89146S), Phospho-Acetyl-CoA Carboxylase Ser79 (3661S), PARP (9542S), Mcl-1 (5453S), survivin (2808S), Cleaved Caspase-3 (9661S), vimentin (5741S), β-Actin (4970S), and NAMPT (86634T)

(18) Phase I first-in-human dose escalation study of the oral casein kinase 1α and cyclin dependent kinase 7/9 inhibitor BTX A51 in advanced MDS and AMLJournal of hematology & oncologyJuly 15, 2025Ball BJ, Xiao W, Borthakur G, Nguyen LXT, Valerio M, Venkatachalam A, Marcucci G, Stein AS, Thai DL, Cook DN, et al.Cell Signaling Cat#9661 s), c-Myc (1:750; Cell Signaling Cat#9402), MCL-1 (1:1000; Cell Signaling Cat#5453), MDM2 (1:500; Cell Signaling #86,934), PP2Ac (1:1000; Cell Signaling #2038), Phospho-Rpb1 CTD (Ser2;

(19) Dendritic cells activate pyroptosis and effector-triggered apoptosis to restrict Legionella infectionmBioJuly 9, 2025Víctor R. Vázquez Marrero, Jessica Doerner, Kimberly A. Wodzanowski, Jenna Zhang, Allyson Lu, Frankie D. Boyer, Isabel Vargas, Suzana Hossain, Karly B. Kammann, Madison V. Dresler, et al.caspase-8 (Cell Signaling 4790), caspase-9 (Cell Signaling 9508), BID (R&D MAB860), Mcl-1 (Cell Signaling 5453), Bcl-XL (Cell Signaling 2764), cFLIP (Cell Signaling 56343), gasdermin-D (abcam 209845), caspase-11

(20) The cancer-associated SF3B1K700E spliceosome mutation confers enhanced sensitivity to BV-6-induced cytotoxicityCell Death DisJuly 1, 2025Lydia E. Roets, Jaine K. Blayney, Hayley P. McMillan, Patrick J. Preston, Alexander M. Mutch, Ken I. Mills, Kienan I. Savage, Katrina M. LappinCaspase-8 (ALX-804-242-C100, Enzo); cIAP1 (10022-1-AP, Proteintech); BCL-XL (2762), FADD (2782), MCL-1 (5453), XIAP (2045), FLIP (56343), and RIP (3493) from cell signalling technology (CST). Anti-mouse (7076)

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Mcl-1 (D35A5) Rabbit mAb

Mcl-1 (D35A5) Rabbit mAb from Cell Signaling Technology

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Mcl-1 (D35A5) Rabbit mAb from Cell Signaling Technology

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