PI3 Kinase p85 (19H8) Rabbit mAb 4257S from Cell Signaling Technology

Product Specs
ItemPI3 Kinase p85 (19H8) Rabbit mAb
CompanyCell Signaling Technology
Price $336.00 Inquire
Catalog Number4257S
Quantity100 µl
ApplicationsW, W-S, IP
ReactivityH, M, R
TargetPI3K p85 alpha
HostRabbit
ClonalityMonoclonal
NCBI Full Gene Namephosphoinositide-3-kinase regulatory subunit 1
NCBI Gene AliasesAGM7, GRB1, IMD36, p85, p85-ALPHA, p85alpha
Research AreaPI3K / Akt Signaling
IsotypeIgG
Clone19H8
Molecular Weight85

Questions and Answers

What applications has the PI3 Kinase p85 antibody been used for in scientific research?

The PI3 Kinase p85 antibody has been shown to work in applications such as Western blotting and immunoprecipitation, as evidenced by its use in various studies to analyze the PI3K/Akt signaling pathway and its role in different cellular contexts.

Can this antibody be used to study the PI3K/Akt/mTOR pathway in cancer research?

Yes, the PI3 Kinase p85 antibody has been utilized in studies investigating the PI3K/Akt/mTOR pathway, including its involvement in drug resistance mechanisms in gastric cancer, as highlighted in research that demonstrated the pathway's activation in paclitaxel-resistant gastric cancer cells ().

Is the PI3 Kinase p85 antibody effective for use in animal models?

The antibody has been effectively used in animal studies, such as in the context of hepatic ischemia/reperfusion injury, where it helped elucidate the role of the PI3K/Akt/mTOR pathway in modulating regulatory T cell function in an acidic microenvironment ().

What species does the PI3 Kinase p85 antibody react with?

The PI3 Kinase p85 antibody is reactive with multiple species, including humans, mice, and rats, making it suitable for a variety of experimental models in cell biology and cancer research.

Cell Signaling Technology

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Lysine Acetyltransferase 6A Drives M1 Macrophage Polarization Through Metabolic Reprogramming in Sepsis-Induced Acute Lung InjuryBiomoleculesApril 20, 2026Xin Wang, Junlin Chen, Yimei Lai, Yumeng Wang, Kaixia Hu, Mengshi Wu, Niansheng Yang, Yuefang Huangp-AKTS473 (1:2000, Cell Signaling Technology, Cat# 4060S), PI3K (1:1000, Cell Signaling Technology, Cat# 4257T), AKT(1:1000, Cell Signaling Technology, Cat# 4691T), p-mTORS2448 (1:1000, Cell Signaling Technology,

(2) ADAMTS2 Modulates Inflammation, Autophagy, and Barrier Integrity Through PI3K/AKT/mTOR Signaling in LPS-Induced Acute Respiratory Distress SyndromeImmun Inflamm DisApril 20, 2026Song Gao, Ting Lyu, Youhong Quan, Yaling Tao, Wenjin Wang, Xi Xu(CST) Beclin‐1,Rabbit,1:1000,ab207612,Abcam p62/SQSTM1,Rabbit,1:1000,ab109012,Abcam PI3K (p85),Rabbit,1:1000,4257S,CST p‐PI3K (Tyr458),Rabbit,1:1000,4228S,CST AKT,Rabbit,1:1000,9272S,CST p‐AKT (Ser473),Rabbit,1:1000,9271S,CST mTOR,Rabbit,1:1000,2983S,CST p‐mTOR

(3) Stemness and Survival: CD117+/CD133+ Subpopulations Sustain PI3K Signaling and Drive Imatinib Resistance in Head and Neck Mucosal MelanomaCellsApril 19, 2026Sofie-Yasmin Hassan, Simeon Santourlidis, Thomas W. Flanagan, Sarah-Lilly Hassan, He Zhou, Morna F. Schmidt, Claudio Cacchi, Matthias Ferdinand Lammert, Mossad Megahed, Amir Sadegh Yazdi, et al.Rabbit mAb #3074], CD133 [(D2V8Q) XP® Rabbit mAb #64326], anti-CD133 (#64326), c-Kit (#37805), p85 (#4257), and Actin (#8457), and Phospho-PDK1 [Ser241] (#3061) were all purchased from Cell Signaling Technology

(4) PRDX1 promotes clear cell renal cell carcinoma progression by modulating EGFR-dependent AKT pathway activationFront PharmacolApril 17, 2026Zhi Liang, Xiaochun Lin, Shen Zeng, Lili Wu, Yujun Dong, Chen Zhang, Mei Li, Fudi Zhu, Lu Chen, Suiqin NiEGFR (4267S, CST), p-EGFR (Tyr1068, 3777S, CST), AKT (4691T,CST), p-AKT (Ser473, 4060L, CST), PI3K (4257T, CST), p-PI3KP85(Tyr458, 4228S, CST), Snail (3879S, CST), E-cad (3195S, CST), N-cad (13116S, CST), KI67

(5) Jingangteng Capsule Attenuates Ulcerative Colitis via Maintaining the Homeostasis of Intestinal Microbiota and Metabolites, Inhibiting the PI3K-AKT-mTOR Signaling PathwayPharmaceuticals (Basel)April 7, 2026Jing Li, Yue Xiong, Shiyuan Cheng, Dan Liu, Qiong Wei, Xiaochuan Yewere purchased from Wuhan Aibo Teke Biological Technology Co., Ltd. (Wuhan, China). Antibodies PI3K (4257T), p-PI3K (4228T), AKT (4691T), p-AKT (4060T), p-PDK1 (3438T), PTEN (9559T), p-mTOR (5536T) were supplied

(6) Recombinant tissue plasminogen activator protects neurons after intracerebral hemorrhage through activating the PI3K/AKT/mTOR pathwayNeural Regeneration ResearchApril 1, 2026Jie Jing, Shiling Chen, Xuan Wu, Jingfei Yang, Xia Liu, Jiahui Wang, Jingyi Wang, Yunjie Li, Ping Zhang, Zhouping Tang3-kinase regulatory subunit alpha (PI3 kinase p85; rabbit, 1:1000, Cell Signaling Technology, Cat# 4257S, RRID: AB_659889), RAC-alpha serine/threonine-protein kinase (AKT; rabbit, 1:1000, Cell Signaling Technology,

(7) Alkaloids from Evodia rutaecarpa inhibit the occurrence and development of gallbladder cancer in vivo and in vitroScientific ReportsMarch 13, 2026Yijie Li, Shufen Zhou, Hanzheng Xu, Hongye Zhou, Kunqi Sun, Siyang Hu, Ke Xu, Jiahua Yang, Yafeng Chen, Wei Li3879 S, rabbit IgG), ZEB1 (Abcam, ab3829, rabbit IgG), p-PI3K (CST, 4228 S, rabbit IgG), PI3K (CST, 4257 S, rabbit IgG), p-Akt (CST, 2965P, rabbit IgG), Akt (CST, 4691P, rabbit IgG), and β-actin (Proteintech,

(8) Combining lenalidomide with IL-2 family of cytokines enhances activating receptor and perforin/granzyme expression in NK cellsPLoS OneMarch 12, 2026Alexandra Calescibetta, Robert Dalton, Nicole Fortenbery, Grace Ward, Sean Christiansen, Xianghong Chen, Pingyan Cheng, Tiffany Razabdouski, Annelise J. Glode, Nhan Tu, et al.130385, Cell Signaling Technology, Danvers, Massachusetts, United States), PI3K p85 (I9H8, 1:1000, cat# 4257, Cell Signaling Technology, Danvers, Massachusetts, United States, RRID:AB_659889) and beta-actin as

(9) Dual functional properties of a probiotic biofilm-decorated bone substitute to combat infection and promote osteoimmunomodulationBioactive MaterialsMarch 1, 2026Shiyuan Song, Wen Zhang, Hongmei Zhuang, Wei Wei, Shuyu Cheng, Dan Qiao, Yin Xiao, Yangheng Zhang, Fuhua Yanantibodies against Runt-related transcription factor 2 (Runx2) (1:500, #12556, CST, MA, USA), PI3K (1:1000, #4257, CST, MA, USA), p-PI3K (1:1000, #4228, CST,MA, USA), AKT (1:1000, #4691, CST, MA, USA), and p-AKT (1:2000,

(10) The protective up-regulation of metallothionein-2A in intervertebral disc degeneration inhibits nucleus pulposus cell ferroptosis through activation of the PI3K/AKT/mTOR pathwayCell Death DiscoveryFebruary 25, 2026Hao Cai, Huo-liang Zheng, Qi-zhu Chen, Shao-kuan Song, Bing-yi Yang, Yong Wang, Hui Deng, Muradi Mardan, Ze-yu Lu, Peng-bo Chen, et al.1:1000); COL2 (Abcam, ab188570, 1:1000); MMP3 (Abcam, ab52915, 1:1000); PI3K (Cell Signaling Technology, 4257, 1:1000); p-PI3K (Cell Signaling Technology, 4228, 1:1000); AKT (Cell Signaling Technology, 9272, 1:1000);

(11) The mechanisms by which hypothalamic neuroinflammation induced by neonatal cerebral ischemia–hypoxia leads to decreased thymic function via the HPA axisCell & BioscienceFebruary 23, 2026Gai-Gai Liu, Li-Yan Shuang, Qian Zhang, Guang-Jun Su, Yun Huang, Jin-Hua Xue, Li-Xia Jiang, Cheng Huang, Tao Chen, Zhi-Hua Huang, et al.Autophagic proteins Beclin-1 (66665-1, Proteintech), LC3 II/I (2775 S, CST), p-PI3K (ab302958, Abcam), PI3K (4257S, CST), p-Akt (WLP001a, Wanleibio), Akt (4060S, CST), p-mTOR (5536 S, CST), mTOR (2983, CST), GR (WL02695,

(12) USP14 promotes epithelial-mesenchymal transition and functions as a multifaceted biomarker in lung adenocarcinomaDiscover OncologyFebruary 21, 2026Chenzhuo Wu, Zhimeng Chen, Jian Yang, Hao Shi, Yuxuan Xing, Haoran Liu, Ziqing Shen, Chun Xu, Cheng Ding, Hao Ding, et al.E-Cadherin (#3195, Cell Signaling Technology), Vimentin (10366-1-AP, Proteintech), PI3 Kinase p85 (#4257, Cell Signaling Technology), Phospho-PI3K p85 (Tyr458)[Tyr467]/p55 (Tyr199) (#AF3242, Affinity Biosciences),

(13) Pan-cancer N-glycoproteomic atlas of patient-derived xenografts uncovers FAT2 as an actionable surface targetCell Reports MedicineFebruary 17, 2026Meinusha Govindarajan, Salvador Mejia-Guerrero, Shawn C. Chafe, Shahbaz Khan, Wei Shi, Matthew Waas, Martin A. Rossotti, Amanda Khoo, Lydia Y. Liu, Vladimir Ignatchenko, et al.3438; RRID:AB_2161134 PI3 Kinase p85 (19H8) Rabbit monoclonal antibody,Cell Signaling Technology,Cat# 4257; RRID:AB_659889 Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) (E3U1H) Rabbit monoclonal antibody,Cell Signaling Technology

(14) Potential Involvement of PI3K/AKT Signaling Pathway in the Protective Effects of Rhinacanthus nasutus Against Diabetic Nephropathy-Induced Oxidative StressAntioxidants (Basel)February 14, 2026Junyu Liu, Yehao Lin, Xudong Yi, Min Zhang, Pharkphoom Panichayupakaranant, Joseph Buhagiar, Haixia Chenβ-actin (CW0264M) was purchased from CWBIO (Jiangsu, China). The primary antibodies p-Akt (4060S), PI3K (4257S), and Akt (4691S) were obtained from Cell Signaling Technology (Danvers, MA, USA). The p-PI3K primary

(15) PLOD2 promotes proliferation, migration and invasion of colorectal cancer cells via PI3K-AKT-GSK3β signaling pathwayScientific ReportsFebruary 10, 2026Hua Fang, Jing Zheng, Shutong Ren, Danjing Chen, Yunli Wu, Xian-E PengBiotechnology Co., Ltd.), GAPDH antibody (5174,Cell Signaling Technology, Inc., USA), PI3K antibody (4257, Cell Signaling Technology, Inc., USA).Phosoho-PI3K antibody (4228, Cell Signaling Technology, Inc.,

(16) Hydroxychloroquine Prevents High-altitude Cerebral Edema by Inhibiting Endothelial Claudin-5 Autophagic DegradationCurrent NeuropharmacologyFebruary 1, 2026Yan Xue, Baolan Wan, Zhen Wang, Zhiwei Wang, Dongzhi Wang, Wanping Yang, Xueting Wang, Li Zhu18420-1-AP), LC3 (CST, 12741S), ATP1A1 (Abcam, ab300507), mTOR (CST, 2983), p-mTOR (CST, 5536), PI3K (CST, 4257), p-PI3K (CST, 4228), Akt (CST, 4691), p-Akt (CST, 4060), Erk (Abcam, ab184699), p-Erk (Abcam, ab201015),

(17) miR-145-5p/CAMSAP2 axis modulates cisplatin sensitivity in non-small cell lung cancerCancer Cell InternationalJanuary 31, 2026Natsaranyatron Singharajkomron, Wanatchaporn Arunmanee, Sedthawut Laotee, Boon-Cher Goh, Win Lwin Thuya, Chayanan Sodsee, Pharot Khoykaew, Suphong Plangsothorn, Iksen Iksen, Pithi Chanvorachote, et al.anti-phosphorylate PI3K (Cell Signaling Technology #4228, 1:1000), anti-PI3K (Cell Signaling Technology #4257, 1:1000), anti-β-catenin (Cell Signaling Technology #8480, 1:1000), anti-phosphorylate GSK3β (Cell Signaling Technology

(18) Pteryxin enhances human NK-cell cytotoxicity by upregulating NKp30, NKp46, and 2B4 via ERK/AKT signalingFrontiers in PharmacologyJanuary 21, 2026Eun Sun Park, Jong-Tae Kim, Yo-Sep Hwang, Jahyeong Han, Hyo-Min Park, Hyang Ran Yoon, Hee Jun Cho, Hee Gu Lee(cat. no. 4694), anti-human phospho MEK1/2 (Ser257/Thr261) (cat. no. 9154), anti-human PI3K (cat. no. 4257), anti-human phospho PI3K (cat. no. 4228), and anti-human granzyme B (cat. no. 4275) purchased from

(19) Interleukin-33 Promotes Neutrophil Extracellular Trap Formation To Aggravate Renal Ischemia-Reperfusion Injury Through ST2/PI3K/Akt and ST2/PAD4 PathwaysInflammationJanuary 20, 2026Feilong Zhang, Jiyue Wu, Zhen Li, Lijian Gan, Haoyuan Cao, Chaozhen Chen, Zejia Sun, Jiandong Zhang, Hailong Li, Xulong Zhang, et al.ab187060, UK), KIM-1 (2ug/mL, Abcam, ab78494, UK), p-PI3K (1:1000, CST, 4228, America), PI3K (1:1000, CST, 4257, America), p-Akt (1:1000, CST, 9271, America), Akt (1:1000, CST, 9272, America), PAD4 (1:3500, Proteintech,

(20) Pathological neutrophil extracellular traps hinder postoperative anal fistula wound healing and are attenuated by Zuoqing granule via suppression of the Nox4 pathwayFrontiers in ImmunologyJanuary 20, 2026Xiaoli Fang, Heng Deng, Ming Li, Xiang Gao, Chunrong He, Hui Liuat 4°C with primary antibodies against: Nox4 (1:1000, Proteintech, 14347-1-AP), PI3K (1:1000, CST, #4257), p-Akt (Ser473, 1:2000, CST, #4060), Akt (1:1000, CST, #4691), PADI4 (1:1000, Abcam, ab214810), and

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PI3 Kinase p85 (19H8) Rabbit mAb

PI3 Kinase p85 (19H8) Rabbit mAb from Cell Signaling Technology

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PI3 Kinase p85 (19H8) Rabbit mAb from Cell Signaling Technology

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