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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Independent and Complementary Functions of Caf1b and Hir1 for Chromatin Assembly in Tetrahymena thermophilaCellsDecember 13, 2023Huijuan Hao, Chenhui Ren, Yinjie Lian, Min Zhao, Tao Bo, Jing Xu, Wei Wang(dilution ratio of 1:500, #3724S, CST, Danvers, MA, USA), mouse anti-HA (dilution ratio of 1:200, #2367S, CST, Danvers, MA, USA), and rabbit anti-Pcna1 (dilution ratio of 1:500, WG11849, ABclonal, Wuhan, China)

(2) The HACE1 E3 ligase mediates RAC1‐dependent control of mTOR signaling complexesEMBO reportsDecember 6, 2023Busra Turgu, Amal El‐Naggar, Melanie Kogler, Luigi Tortola, Hai‐Feng Zhang, Mariam Hassan, Michael M Lizardo, Sonia HY Kung, Wan Lam, Josef M Penninger, et al.Anti‐HACE1 antibody [EPR7962] (Abcam, ab133637); Anti‐HA antibody mouse mAb (Cell Signaling Technology #2367, RRID:AB_10691311); Anti‐HA antibodyRabbit mAb (Cell Signaling Technology, Cat# 3724, RRID:AB_1549585);

(3) Noncanonical autophagy is a new strategy to inhibit HSV-1 through STING1 activationAutophagyDecember 2, 2023Zhihua Zheng, Man Zhao, Hao Shan, Dongmei Fang, Zuyi Jin, Jiuge Tang, Zhiping Liu, Liang Hong, Peiqing Liu, Min Lip-IRF3 (Ser396, 4D4G), LAMP1 (D2D11), ATG13 (E1Y9V), ATG9A (D409D), BECN1 (3738S), CANX (2679P), HA (2367S), and ATG4B (D1G2R) were from Cell Signaling Technology; antibodies against LC3B (L7543), ATG5 (A0731),

(4) The subset of peroxisomal tail-anchored proteins do not reach peroxisomes via ER, instead mitochondria can be involvedPLoS OneDecember 1, 2023Tamara Somborac, Güleycan Lutfullahoglu Bal, Kaneez Fatima, Helena Vihinen, Anja Paatero, Eija Jokitalo, Ville O. Paavilainen, Svetlana Konovalovarabbit anti-ACBD5 1:500 (Atlas Antibodies, HPA012145), mouse anti-HA 1:1000 (Cell Signaling Technology, 2367), mouse anti-β-actin, 1:1000 (Cell Signaling Technology, 3700), IRDye 800CW conjugated streptavidin

(5) SARS-CoV-2 nonstructural protein 6 triggers endoplasmic reticulum stress-induced autophagy to degrade STING1AutophagyDecember 1, 2023Jiao P, Fan W, Ma X, Lin R, Zhao Y, Li Y, Zhang H, Jia X, Bi Y, Feng X, et al.(Ser172) (5483), IRF3 (11904), phospho-IRF3 (Ser396) (4947), Flag (14793), MYC-Tag (2276), HA-Tag (2367), GFP (2955), RETREG1/FAM134B (61011), CCPG1 (80158), and GAPDH (97166) were purchased from Cell Signaling Technology

(6) The formation of ubiquitin rich condensates triggers recruitment of the ATG9A lipid transfer complex to initiate basal autophagybioRxivNovember 30, 2023David G Broadbent, Colten M McEwan, Tsz-Ming Tsang, Daniel M Poole, Bradley C Naylor, John C Price, Jens Christopher Schmidt, Joshua L Andersenrabbit,Cell Signaling Technology,48046 (Product discontinued) Anti-HA-Tag mouse,Cell Signaling Technology,2367S Anti-HA-Tag goat,Novus Biologicals,NB600-362 NBR1,Cell Signaling Technology,D2E6 NDP52,Cell Signaling

(7) Transcriptional activation of the Myc gene by glucose in β-cells requires a ChREBP-dependent 3-D chromatin interaction between the Myc and Pvt1 genesMolecular MetabolismNovember 30, 2023Liora S. Katz, Gabriel Brill, Peng Wang, Luca Lambertini, Pili Zhang, Jonathan M. Haldeman, Hongtao Liu, Christopher B. Newgard, Andrew F. Stewart, Adolfo Garcia-Ocaña, et al.Ki67 (ThermoScientific, MA5-14520, 1:250), insulin (GeneTex, GTX27842, 1:1000), or HA (Cell Signaling, 2367S, 1:250). Confocal images were acquired using an Agilent BioTek Cytation 10 confocal imager. 4.4 Chromosome

(8) Mitoguardin 1 and 2 promote granulosa cell proliferation by activating AKT and regulating the Hippo-YAP1 signaling pathwayCell Death & DiseaseNovember 27, 2023Ming-Qi Yan, Bing-Hong Zhu, Xiao-Hong Liu, Yu-Meng Yang, Xiu-Yun Duan, Yong Wang, Hui Sun, Mei Feng, Tao Li, Xiao-Man Liuconcentration,Description FLAG,AB_10950495,Cell Signaling Technology,8146,WB: 1:1000,Mouse monoclonal HA,AB_10691311,Cell Signaling Technology,2367,WB: 1:1000,Mouse monoclonal pAKT (S473),AB_2315049,Cell Signaling Technology,4060,WB: 1:1000,Rabbit

(9) Unveiling the pathogenic mechanisms of NPR2 missense variants: insights into the genotype-associated severity in acromesomelic dysplasia and short statureFrontiers in Cell and Developmental BiologyNovember 23, 2023Sally Badawi, Divya Saro Varghese, Anjana Raj, Anne John, Hamda S. Al-Musafir, Ahmed J. Al-Ghamari, Alreem R. Alshamsi, Sara H. Ouda, Ghayth Al-Dirbashi, Bassam R. Alithen probed with the corresponding primary antibodies: anti-HA (1:2000 Cell Signaling Technology, cat# 2367S), anti-GAPDH antibody (1:1000 Cell Signaling Technology, cat# 2118S) and their respective secondary

(10) Expression of the kidney anion exchanger 1 affects WNK4 and SPAK phosphorylation and results in claudin-4 phosphorylationHeliyonNovember 13, 2023Rawad Lashhab, Grace Essuman, Maria Chavez-Canales, R. Todd Alexander, Emmanuelle CordatMonoclonal mouse anti-HA antibody was purchased from BioLegend (Cat # 901513) or Cell Signalling (Cat # 2367). 2.2 Cell culture and transient transfection Immortalized mouse inner medullary collecting duct cells

(11) Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxivNovember 11, 2023Megan D. Schertzer, Andrew Stirn, Keren Isaev, Laura Pereira, Anjali Das, Claire Harbison, Stella H. Park, Hans-Hermann Wessels, Neville E. Sanjana, David A. Knowlesusing western blotting and immunofluorescence methods with an anti-HA antibody (Cell Signaling Cat #2367). Cas13 knockdown activity was confirmed by FACS using CD46 as a positive control (Cell Signaling Cat

(12) An Adapted GeneSwitch Toolkit for Comparable Cellular and Animal Models: A Proof of Concept in Modeling Charcot-Marie-Tooth NeuropathyInternational Journal of Molecular SciencesNovember 9, 2023Laura Morant, Maria-Luise Petrovic-Erfurth, Albena Jordanovafollowing antibodies were used in different Western blot experiments: mouse monoclonal HA-tag (1:1000, 2367S, Cell Signaling Technology, Beverly, MA, USA), mouse monoclonal YARS1 (1:1000, H00008565-M02A, Abnova,

(13) The SGK3-Catalase antioxidant signaling axis drives cervical cancer growth and therapy resistanceRedox BiologyNovember 1, 2023Min Wang, Jiannan Liu, Xingming Liao, Yasong Yi, Yijue Xue, Ling Yang, Hailing Cheng, Pixu Liu(CST#12980, 1:1000), DYKDDDDK tag (Proteintech# 66008-4-Ig, 1:1000), HA-tag (CST#3724, 1:1000), HA-tag (CST#2367, 1:100), EEA1 (Santa Cruz# sc-137130,1:300). 4.13 Cell viability assays Cells were seeded in 96-well

(14) KDM5-mediated activation of genes required for mitochondrial biology is necessary for viability in DrosophilaDevelopmentNovember 1, 2023Michael F. Rogers, Owen J. Marshall, Julie Secombewashed, and immediately mounted. The following primary antibodies were used: mouse anti-HA (1:100; 2367, Cell Signaling Technology) and rabbit anti-GFP (1:100; A11122, Invitrogen). Primary antibodies were

(15) NRF2/ARE mediated antioxidant response to glaucoma: role of glia and retinal ganglion cellsActa Neuropathologica CommunicationsOctober 24, 2023Sarah Naguib, Jon R. Backstrom, Elisabeth Artis, Purnima Ghose, Amy Stahl, Rachael Hardin, Ameer A. Haider, John Ang, David J. Calkins, Tonia S. Rex(Rockland Immunochemicals, Inc., Limerick, PA; #600-401-379) and mouse anti-HA (Cell Signaling Technologies, Danvers, MA; #2367). ARE activation, based on increased tdTomato (tdTom) fluorescence, was detected in transfected cells

(16) Palmitoyltransferase DHHC9 and acyl protein thioesterase APT1 modulate renal fibrosis through regulating β-catenin palmitoylationNature CommunicationsOctober 21, 2023Mengru Gu, Hanlu Jiang, Mengzhu Tan, Long Yu, Ning Xu, Ying Li, Han Wu, Qing Hou, Chunsun Daiantibody produced in rabbit (1:500; cat: SAB4502104, Sigma-Aldrich), HA-Tag (6E2) Mouse mAb (1:1000; cat: 2367S, Cell signaling technology), GAPDH polyclonal antibody (1:3000; cat: AP0063, Bioworld, Nanjing, China),

(17) Time-resolved proximity biotinylation implicates a porin protein in export of transmembrane malaria parasite effectorsJournal of Cell ScienceOctober 15, 2023David Anaguano, Watcharatip Dedkhad, Carrie F. Brooks, David W. Cobb, Vasant MuralidharanWashington University School of Medicine, St Louis, MO 63110, USA; 1:2000), and mouse-anti-HA 6E2 (cat. no 2367, Cell Signaling Technology, 1:2000). Secondary antibodies used were IRDye 680 CW goat-anti-rabbit IgG,

(18) FORMATION OF MALIGNANT, METASTATIC SMALL CELL LUNG CANCERS THROUGH OVERPRODUCTION OF cMYC PROTEIN IN TP53 AND RB1 DEPLETED PULMONARY NEUROENDOCRINE CELLS DERIVED FROM HUMAN EMBRYONIC STEM CELLSbioRxivOctober 14, 2023Huanhuan Joyce Chen, Eric E. Gardner, Yajas Shah, Kui Zhang, Abhimanyu Thakur, Chen Zhang, Olivier Elemento, Harold VarmusSc-126), anti-MYC (Cell Signaling, clone D84C12, Cat# 5605), anti-HA (Cell Signaling, clone 6E2, Cat#2367), and anti-GAPDH (Cell Signaling, clone 14C10, Cat# 2118). All antibodies for Western Blot were used

(19) Pathogenicity and functional analysis of CFAP410 mutations causing cone-rod dystrophy with macular staphylomaFrontiers in MedicineOctober 12, 2023Shaoqing Yang, Ya Li, Lin Yang, Qingge Guo, Ya You, Bo Leimembranes were blocked with 5% skim milk and incubated with primary antibodies mouse anti-Flag (1:5,000; 2367S, Cell Signaling Technology, MA, United States) and rabbit anti-βactin (1:5,000; 4970S, Cell Signaling Technology

(20) Fasting‐sensitive SUMO‐switch on Prox1 controls hepatic cholesterol metabolismEMBO reportsOctober 9, 2023Ana Jimena Alfaro, Claudia Dittner, Janina Becker, Anne Loft, Amit Mhamane, Adriano Maida, Anastasia Georgiadi, Foivos‐Filippos Tsokanos, Katarina Klepac, Claudia‐Eveline Molocea, et al.Melchior lab), anti‐β actin (Sigma Aldrich—A5441), anti‐Vcp (Abcam—ab11433), anti‐HA tag (Cell Signaling—2367), anti‐tubulin (Santa Cruz—5274) and anti‐RCC1 (BD Biosciences—610377) antibodies. Blots were analyzed

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(1)

6/16/2023Anti-HA Tag Antibody Works Well Liyong Z.Used to identify an HA-tag primary antibody to detect the exogenous expression of the constructed plasmids in OE19 stable cell models, five HA-tag primary antibodies (Cat # GTX628489, 3724S, 2367S, ... Read Review
(1)

2/7/2020Western Blot Analysis of Extracts from Stem Cells Expressing HA-Tagged LacZ Protein with Anti-HA Antibody Raghavendra VadlaGood antibody for western blot analysis.
(1)

7/17/2015This Antibody Can be Used for Immunohistochemistry and Western Blot Study junyan taoI do cloning for animal experiments to use as liver cancer models and thus use a variety of molecular biology reagents including antibodies, plasmids, and RNA kits. For this antibody, just follow the ... Read Review
(1)

4/9/2015Very good HA antibody Benjamin GerovacI used this anti-HA antibody to stain my N-terminal HA tagged NICD3 protein. NICD3 should primarily be in the nucleus and I see nice nuclear staining with the HA antibody. Overall, this is a good ... Read Review

HA-Tag (6E2) Mouse mAb

HA-Tag (6E2) Mouse mAb from Cell Signaling Technology

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Cell Signaling Technology

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HA-Tag (6E2) Mouse mAb from Cell Signaling Technology

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