HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY IKBKB (Cat# RC219154, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IKBKB antibody (Cat# TA890128). Positive lysates [LY419865] (100ug) and [LC419865] (20ug) can be purchased separately from OriGene.