Myc-Tag (9B11) Mouse mAb (Alexa Fluor ® 647 Conjugate) 2233S from Cell Signaling Technology

Product Specs
ItemMyc-Tag (9B11) Mouse mAb (Alexa Fluor ® 647 Conjugate)
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number2233S
Quantity100 µl
ApplicationsIF-IC, FC-FP
ReactivityAll
TargetMyc Tag
HostMouse
Conjugate/TagAlexa Fluor ® 647
ClonalityMonoclonal
Research AreaRelated Products
Clone9B11

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) The anti-SARS-CoV-2 effect and mechanism of Chiehyuan herbal oral protection solutionHeliyonJuly 3, 2023Ching-Yuan Wu, Yao-Hsu Yang, Yu-Shih Lin, Li-Hsin Shu, Yu-Ching Cheng, Hung-Te Liu, Yin-Yin Lin, I-Yun Lee, Wei-Tai Shih, Pei-Rung Yang, et al.medium composed of RBD-sfGFP and anti-MYC Alexa 647 (clone 9B11, Cell Signaling Technology, Cat. Number: 2233S). Lastly, the cells were washed twice with PBS-BSA and analyzed by a flow cytometer BD FACSCanto (Becton

(2) Tuning charge density of chimeric antigen receptor optimizes tonic signaling and CAR-T cell fitnessCell researchMay 1, 2023Chen J, Qiu S, Li W, Wang K, Zhang Y, Yang H, Liu B, Li G, Li L, Chen M, et al.anti-ICOS-PE-CY7 (1:800, Biolegend, 313520), anti-Myc-Alexa Fluor 647 (1:1000, Cell Signaling Technology, 2233 S), anti-PD-1-PE (1:800, Biolegend, 367404), anti-LAG-3-PE-Cy7 (1:800, eBioscience, 25-2239-42), anti-TIM-3-PerCP-Cy5.5

(3) How T118M peripheral myelin protein 22 predisposes humans to Charcot–Marie–Tooth diseaseJournal of Biological ChemistryFebruary 1, 2023Katherine M. Stefanski, Geoffrey C. Li, Justin T. Marinko, Bruce D. Carter, David C. Samuels, Charles R. Sanderslabeled with an anti–myc antibody conjugated to Alexa Fluor 647 (Cell Signaling Technology; catalog no.: 2233). Cells were then analyzed via flow cytometry on a BD LSRFortessa (Becton, Dickinson and Company). Cells

(4) Programming multicellular assembly with synthetic cell adhesion moleculesNatureFebruary 1, 2023Stevens AJ, Harris AR, Gerdts J, Kim KH, Trentesaux C, Ramirez JT, McKeithan WL, Fattahi F, Klein OD, Fletcher DA, et al.anti-MYC-tag (9B11) mouse monoclonal antibodies (AlexaFluor 647 conjugate) (Cell Signaling Technology, 2233, 25, 1:100); anti-HA-tag (6E2) mouse monoclonal antibodies (AlexaFluor 647 conjugate) (Cell Signaling Technology

(5) Deep mutational scanning identifies SARS-CoV-2 Nucleocapsid escape mutations of currently available rapid antigen testsCellSeptember 15, 2022Filipp Frank, Meredith M. Keen, Anuradha Rao, Leda Bassit, Xu Liu, Heather B. Bowers, Anamika B. Patel, Michael L. Cato, Julie A. Sullivan, Morgan Greenleaf, et al.63730S; RRID:AB_2645040 Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate),Cell Signaling,Cat# 2233; RRID:AB_823474 Goat anti-Rabbit IgG,ThermoFisher,P-2771MP; RRID:AB_2539845 Goat anti-Mouse IgG,ThermoFisher,P-852;

(6) Sentinel cells enable genetic detection of SARS-CoV-2 Spike proteinbioRxivAugust 12, 2022Zara Y. Weinberg et al.proteins were assayed for using Alexa Fluor 647 Anti-Myc tag antibody (Cell Signaling Technologies #2233S). Jurkat activation was assessed using Alexa Fluor 647 anti-CD25 (Biologend #302618) and BUV395 anti-CD69

(7) Recursive Editing improves homology-directed repair through retargeting of undesired outcomesNature communicationsAugust 5, 2022Möller L, Aird EJ, Schröder MS, Kobel L, Kissling L, van de Venn L, Corn JE.(Clone IP26; BioLegend 306722) and 1:50 of Alexa Fluor 647 anti-myc tag (Clone 9B11; Cell Signaling 2233). Samples were analyzed using an Attune NxT Flow Cytometer (Thermo Fisher), software v3.2.1. Data were

(8) Sweet taste receptor agonists attenuate macrophage IL-1β expression and eosinophilic inflammation linked to autophagy deficiency in myeloid cellsClinical and translational medicineAugust 1, 2022Lee J, Kim SJ, Choi GE, Yi E, Park HJ, Choi WS, Jang YJ, Kim HS.T1R3, THP‐1 cells were stained with anti‐cMyc antibody‐Alexa Fluor 647 (Cell Signaling Technology, 2233s; 1:50) and measured by Accuri C6 flow cytometer (BD Biosciences). FlowJo software (Tree Star, Ashland,

(9) Proteome-Scale Screening to Identify High-Expression Signal Peptides with Minimal N-Terminus Biases via Yeast DisplayACS Synthetic BiologyJuly 15, 2022Patrick V. Holec, Karen V. Camacho, Kathryn C. Breuckman, Jody Mou, Michael E. Birnbaumat 20°C for 48 hours. Yeast were separately labeled with anti-Myc (Cell Signaling Technologies, C/N: 2233S, clone: 9B11) and anti-β2m (AbCam, C/N: ab187602, clone: B2M01) antibodies containing a conjugated Alexa

(10) A high-throughput yeast display approach to profile pathogen proteomes for MHC-II bindingeLifeJuly 4, 2022Brooke D Huisman, Zheng Dai, David K Gifford, Michael E Birnbaumanti-Myc-AlexaFluor647 antibody (1:100 volume:volume; Myc tag (9B11) Mouse mAb AlexaFluor647 Conjugate #2233 Cell Signaling Technology) and anti-AlexaFluor647 magnetic beads (1:10 volume:volume) and flowed over

(11) De novo-designed transmembrane domains tune engineered receptor functionseLifeMay 4, 2022Assaf Elazar et al.(1:800) Antibody,Alexa Fluor 647 anti-human c-Myc-tag mouse IgG2a monoclonal antibody(clone: 9B11),Cell Signaling,Cat# 2233S,Flow cytometry (neat) Antibody,APC anti-mouse CD28 Syrian hamster IgG monoclonal antibody(clone: 37.51),BioLegend,Cat#

(12) Modular design of synthetic receptors for programmed gene regulation in cell therapiesCellApril 14, 2022Iowis Zhu, Raymond Liu, Julie M. Garcia, Axel Hyrenius-Wittsten, Dan I. Piraner, Josef Alavi, Divya V. Israni, Bin Liu, Ahmad S. Khalil, Kole T. Roybalmyc-tag (EQKLISEEDL) for easy determination of surface expression with α-myc AF647 (Cell-Signaling #2233). The receptors were cloned into a modified pHR’SIN:CSW vector containing a PGK promoter for all primary

(13) High recombination rate of hepatitis C virus revealed by a green fluorescent protein reconstitution cell systemVirus EvolutionJanuary 29, 2022Andrea Galli, Ulrik Fahnøe, Jens Bukhphycoerythrin (αFlag-PE; 637310, Biolegend) and mouse anti-myc conjugated to AlexaFluor647 (αMyc-647; 2233S, Cell Signaling Tech.); detection was performed using fluorescein (FITC) channel for GFP, phycoerythrin

(14) GB-2 blocking the interaction between ACE2 and wild type and mutation of spike protein of SARS-CoV-2Biomedicine & PharmacotherapyOctober 1, 2021Ming-Shao Tsai et al.containing RBD-sfGFP and the anti-MYC Alexa 647 (clone 9B11, Cell Signaling Technology, Cat. Number: 2233 S). Then, cells were washed twice with PBS-BSA and analyzed by the flow cytometer BD FACSCanto (Becton

(15) Endosomal cAMP production broadly impacts the cellular phosphoproteomeJournal of Biological ChemistryJuly 1, 2021Nikoleta G. Tsvetanova, Michelle Trester-Zedlitz, Billy W. Newton, Grace E. Peng, Jeffrey R. Johnson, David Jimenez-Morales, Andrew P. Kurland, Nevan J. Krogan, Mark von Zastrowand 10 mM stocks, respectively. Alexa 647-conjugated mouse anti-myc antibody (Cell Signaling, Cat #2233S) was used at 1:50. Rabbit anti-phospho-PKA substrate (100G7E) antibody was purchased from Cell Signaling

(16) SynNotch-CAR T cells overcome challenges of specificity, heterogeneity, and persistence in treating glioblastomaScience translational medicineApril 28, 2021Choe JH, Watchmaker PB, Simic MS, Gilbert RD, Li AW, Krasnow NA, Downey KM, Yu W, Carrera DA, Celli A, et al.(EQKLISEEDL) or flag-tag (DYKDDDDK) for detecting surface expression with α-myc A647 (Cell Signaling, no. 2233) or α-flag A647 (R&D Systems, no. IC8529R); see Morsut et al. (16) for synNotch sequence. Receptors

(17) T cells selectively filter oscillatory signals on the minutes timescaleProceedings of the National Academy of Sciences of the United States of AmericaMarch 2, 2021O'Donoghue GP, Bugaj LJ, Anderson W, Daniels KG, Rawlings DJ, Lim WA.determination of surface expression for sorting with α-myc-Alexa647 (clone 9B11, Cell Signaling Technologies, #2233S) and α-DYKDDDDK-Alexa488 (clone 1042E, R&D Systems, #IC8529G-100) antibodies. Staining with α-Myc and

(18) Disease-linked supertrafficking of a potassium channelJournal of Biological ChemistryFebruary 16, 2021Hui Huang, Laura M. Chamness, Carlos G. Vanoye, Georg Kuenze, Jens Meiler, Alfred L. George, Jonathan Patrick Schlebach, Charles R. Sanderswith 1:100 diluted Alexa Fluor 647–conjugated anti-myc (9B11) mouse monoclonal antibody (catalog no. 2233; Cell Signaling Technology) and incubated for 30 min in the dark. Cells were washed once, and fluorescence

(19) Reversible ON- and OFF-switch chimeric antigen receptors controlled by lenalidomideScience Translational MedicineJanuary 6, 2021Max Jan et al.CAR T cells were then assessed by flow cytometry for CAR (anti-Myc tag; Cell Signaling Technology, 2233) and CD69 abundance (Biolegend, 310920). Normalized CAR abundance was calculated by subtraction of the

(20) CD103⁺ cDC1 and endogenous CD8⁺ T cells are necessary for improved CD40L-overexpressing CAR T cell antitumor functionNature communicationsDecember 2, 2020Kuhn NF, Lopez AV, Li X, Cai W, Daniyan AF, Brentjens RJ.BioLegend 107635, RRID:AB_2561397, 0.2 µg/ml; anti-human Myc-tag (9B11) AlexaFluor 647 Cell Signaling 2233 S, RRID:AB_10693328, 1:500. Quantification of total cell numbers by flow cytometry was done using 123count

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11/8/2023Myc Tag (9B11) Mouse mAb From Cell Signaling Works Well for Protein Detection Matthew KimAntibody was used for staining tagged membrane proteins for FAC isolation and for expression level profiling by flow cytometry. The product was very good in both applications.

Myc-Tag (9B11) Mouse mAb (Alexa Fluor ® 647 Conjugate)

Myc-Tag (9B11) Mouse mAb (Alexa Fluor ® 647 Conjugate) from Cell Signaling Technology

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Cell Signaling Technology

Citations (132)

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Myc-Tag (9B11) Mouse mAb (Alexa Fluor ® 647 Conjugate) from Cell Signaling Technology

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