HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DUSP1 (RC205220, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DUSP1 r. Positive lysates LY401403 (100ug) and LC401403 (20ug) can be purchased separately from OriGene.