HER2/ErbB2 (29D8) Rabbit mAb 2165S from Cell Signaling Technology

Product Specs
ItemHER2/ErbB2 (29D8) Rabbit mAb
CompanyCell Signaling Technology
Price $347.00 Inquire
Catalog Number2165S
Quantity100 µl
ApplicationsW, IP, IHC-P, IF-IC, FC-FP
ReactivityH, M
TargetHER2/ErbB2
HostRabbit
NCBI Gene AliasesCD340, HER-2, HER-2/neu, HER2, MLN 19, MLN-19, NEU, NGL, TKR1, VSCN2, c-ERB-2, c-ERB2, p185(erbB2)
ClonalityMonoclonal
NCBI Full Gene Nameerb-b2 receptor tyrosine kinase 2
Research AreaRTK
IsotypeIgG
Clone29D8
Molecular Weight185 kDa

Questions and Answers

Can this antibody be used for studying the role of HER2 in breast cancer?

Yes, the HER2/ErbB2 (29D8) Rabbit mAb has been utilized in various studies investigating the role of HER2 in breast cancer. For instance, it has been shown to facilitate HER2-induced mammary tumorigenesis through multiple signaling pathways, including the PTEN/phosphoinositide-3-kinase/AKT axis, as reported in the study by Cai-Mcrae et al. ().

Is this antibody effective for immunohistochemistry applications?

The HER2/ErbB2 (29D8) Rabbit mAb has been effectively used in immunohistochemistry, as demonstrated in studies where it was employed to assess HER2 expression in various cancer tissues, including breast cancer and gastric cancer ().

What species can this antibody detect, and has it been cited in relevant research?

This antibody is reactive with human and mouse samples. It has been cited in over 190 scientific articles, indicating its widespread use and relevance in cancer research, particularly in studies focusing on receptor tyrosine kinases and their roles in tumor biology.

How does this antibody contribute to understanding HER2's function in cellular signaling?

The HER2/ErbB2 (29D8) Rabbit mAb has been used to elucidate the mechanisms by which HER2 regulates cellular processes, such as autophagy. For example, research has shown that nuclear ErbB2 can repress DEPTOR transcription, thereby activating the mTOR pathway and inhibiting autophagy, highlighting its role as a transcriptional regulator ().

Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) A degrader of HER2 and EGFR abolishes p95HER2 and shows robust antitumor efficacy in HER2-positive breast cancerSci RepApril 20, 2026Lu Yang, Arup Bhattacharya, Yun Li, Darrell Peterson, Yuesheng Zhangfrom Cell Signaling Technology: Anti-EGFR (Cat # 2232), anti-pY1068-EGFR (Cat # 2236), anti-HER2 (Cat# 2165), anti-pY1221/1222-HER2 (Cat # 2243), anti-MET (Cat # 8198), anti-pY1234/1235-MET (Cat #gene 3077),

(2) Payload Diversification Overcomes Resistance and Guides Sequential Antibody–Drug Conjugate Therapy in Breast CancerClinical Cancer ResearchApril 15, 2026Dileep Reddy Rampa, Minji Seo, Nanae Ogata, Zhaoxuan Yang, Nithya Sridhar, Takeo Fujii, Chalothorn Wannaphut, Jennifer A. Maynard, Kyoji Tsuchikama, George W. Sledge, et al.Blots were probed with the following primary antibodies (1:1000): anti-HER2 (Cell Signaling Technology #2165, RRID: AB_10692490), anti-Trop2 (Abcam #ab214488, RRID: AB_2811182), anti-BCRP (Proteintech #27286–1-AP,

(3) Tissue-specific inflammation induces cell state plasticity with oncogenic addiction in mucosal melanomaSci AdvApril 3, 2026Xuhui Ma, Yanni Ma, Li Zhang, Ruixin Liu, Ronghui Xia, Meiling Hao, Xiaole Song, Yinan Chen, Yang Zheng, Hao Wang, et al.,Cell Signaling,4791 Anti-Phospho-HER2/ErbB2 (Tyr1221/1222),Cell Signaling,2243 Anti-HER2/ErbB2,Cell Signaling,2165 Anti-HER3/ErbB3,Cell Signaling,12708 Anti-Phospho-STAT3 (Tyr705),Cell Signaling,9145 Anti-GP100,HUABIO,HA721719 Anti-Phospho-NF-κB

(4) IFNγ-induced antigen loss in chimeric antigen receptor-T cell therapyFront ImmunolMarch 17, 2026Miao Cao, Jasmine Alvarez, Ramkrishna Mitra, Michael Xu, Trevor R. Baybutt, Zhengyang Sun, Oluwatobiloba Taylor, Allison S. Doermann, Ross E. Staudt, Scott A. Waldman, et al.Cell Signaling Technology) anti-human CDH17 (88594T, Cell Signaling Technology), and anti-human HER2 (2165T, Cell Signaling Technology). An HRP-conjugated Goat anti-Rabbit or anti-Mouse IgG secondary antibody

(5) IFNγ-induced antigen loss in chimeric antigen receptor-T cell therapyFrontiers in ImmunologyMarch 17, 2026Miao Cao, Jasmine Alvarez, Ramkrishna Mitra, Michael Xu, Trevor R. Baybutt, Zhengyang Sun, Oluwatobiloba Taylor, Allison S. Doermann, Ross E. Staudt, Scott A. Waldman, et al.Cell Signaling Technology) anti-human CDH17 (88594T, Cell Signaling Technology), and anti-human HER2 (2165T, Cell Signaling Technology). An HRP-conjugated Goat anti-Rabbit or anti-Mouse IgG secondary antibody

(6) Quantitative HER2 tissue and plasma profiling predicts the activity of trastuzumab deruxtecan for breast cancernpj Precision OncologyMarch 13, 2026Paolo Tarantino, Se-Eun Kim, Melissa E. Hughes, Ross J. Kusmick, Kalie Smith, Fara Brasó-Maristany, Nay Nwe Nyein Chan, Laia Paré Brunet, Laura Alder, Diana Garcia-Cortes, et al.and with BSA for 30 min, 1 h incubation with primary rabbit monoclonal HER2 antibody (clone 29D8, #2165, IgG, Cell Signaling) at optimal concentration of 1ug/ml mixed together with 1:100 concentration of

(7) Investigating PAK inhibition in combination with PD-1 blockade to enhance cytotoxic CD8+ T cell-mediated killing and suppress invasion of ovarian cancer cellsBritish Journal of CancerMarch 6, 2026Alexandria R. Mitchell, Yiran Chen, Giuseppe Pugliese, Gabriela Vino Flores, David M. Davies, John Maher, Claire M. Wells(PAK7),Protein Tech,66961-1-1g,Mouse,1:1000 PAK6,Gene Tex,GTX127915,Rabbit,1:1000 HER2 (29D8),Cell Signaling,2165,Rabbit,1:1000 HER4 (111B2),Cell Signaling,4795,Rabbit,1:500 β-Catenin (D10A8) XP®,Cell Signaling,8480S,Rabbit,1:1000 pβ-catenin

(8) An MIEN1-based hexamer peptide (LA3IK) inhibits EGF-driven oncogenic signaling in prostate cancer by disrupting EGFR–ERBB2 heterodimerizationScientific ReportsMarch 5, 2026Amit K. Tripathi, Nafees Ahamad, Antariksh Tyagi, Benedicta Quainoo, Adam W. Smith, Pragati Singh, Nirupama Sabnis, Jamboor K. Vishwanathaantibody against EGFR (Cat# 2256S) and a human-specific HER2/ErbB2 (29D8) rabbit monoclonal antibody (Cat# 2165S), both from Cell Signaling Technology, were used to distinguish the closely migrating protein bands

(9) A One-Step Workflow for Size-Based Separation of Extracellular Vesicles With Integrated Surface Marker DetectionJ Extracell BiolMarch 3, 2026Lien Lippens, Niké Guilbert, Sofie Van Dorpe, Sarah Deville, Robin Boiy, Quentin Roux, Nicolaas Lumen, Ilkka Miinalainen, Pekka Rappu, Katrien Vandecasteele, et al.(1:1000) (cat no. 2929S, Cell Signaling Technology), rabbit monoclonal anti‐HER2 (1:1000) (cat no. 2165S, Cell Signaling Technology), mouse monoclonal anti‐GAPDH (1:2500) (cat no. G8795, Merck Life Science),

(10) Construction and application of a genetically engineered mouse model of gastric adenocarcinomaJournal of the Chinese Medical AssociationMarch 1, 2026Yelizhati Madeti, Xiaoyi Chong, Xiaotian Zhangthe following antigens: Cre recombinase (#15036), programmed death ligand-1 (PD-L1) (#64988), HER2 (#2165) (all from CST, Boston, MA), annexin A10 (#66869, Proteintech, Wuhan, China), KrasG12D (#GTX635362,

(11) Comparison of the Efficacy of 35 Anticancer Drugs According to Genomic Profiling and Biological Characteristics of 14 Gastric Cancer Cell LinesInternational Journal of Molecular SciencesFebruary 25, 2026Sung-Hwa Sohn, Hee Jung Sul, Bum Jun Kim, Dae Young ZangAbcam), anti-Frizzled 5 (ab75234; 1:1000; Abcam), anti-CLDN18 (#38-800; 1:1000; Invitrogen), anti-HER2 (#2165; 1:1000; CST), anti-CDK12 (ab317746; 1:1000; Abcam), anti-VEGFR2 (#9698; 1:1000; CST), anti-PD-L1 (#13684;

(12) Oncogenic alterations in PI3K signaling emulated optogenetically recapitulates some phenotypic changes in mammary epitheliaACS Synthetic BiologyFebruary 20, 2026Keith A. Gagnon, Veronica W. Hui, Terry Ching, Amy E. Stoddard, Esther Koh, Jeroen Eyckmans, Ahmad S. Khalil, Christopher S. Chen(C29F4,#3724), anti-pAkt (Ser473) (#9271), HRP-conjugated donkey anti-mouse (#7076, HER2/ErbB2 (29D8, #2165) and anti-GAPDH (14C10, #2118) were purchased from Cell Signaling Technologies. DAPI was from Santa Cruz biotechnology

(13) Trastuzumab Deruxtecan (T-DXd) Resistance via Loss of HER2 Expression and BindingCancer DiscoveryFebruary 6, 2026Wanyi Chen, Avantika Gupta, Nicholas Mai, Sharanya Nag, Joshua S. Lau, Sukrit Singh, John D. Chodera, Bo Liu, Elisa de Stanchina, Fresia Pareja, et al.standard protocol. Membranes were probed using anti-HER2 rabbit mAb 29D8 (Cell Signaling Technology, cat. #2165, RRID: AB_10692490), anti–β-actin 13E5 (Cell Signaling Technology, cat. #4970, RRID: AB_2223172), anti-TROP2

(14) ERBB2 I655V mutation correlates with efficacy of immunotherapy in gallbladder cancerWorld Journal of Surgical OncologyFebruary 4, 2026Qiang Gao, Ying-nan You, Shuo Lian, Chang Pan, Zong-li Zhang, Kang-shuai Liaccording to the manufacturer’s protocol. The following primary antibodies were used: ERBB2 (CST, #2165, 1:1000); PD-L1 (Abclonal, A19135, 1:1000); Phospho-AKT (Ser473) (CST, #4060, 1:1000); AKT (CST, #9272,

(15) Ebastine targets HER2/HER3 signaling and cancer stem cell traits to overcome trastuzumab resistance in HER2‑positive breast cancerInt J Mol MedFebruary 2, 2026Eunsun Jung, Ji Young Kim, Dongmi Ko, Juyeon Seo, Sang Yoon Lee, Daeun Lee, Subeen Kim, Minsu Park, Seongjae Kim, Soeun Park, et al.no. ab692) and CD44 (all Abcam; cat. no. ab254530); HER2 (Cell Signaling Technology, Inc.; cat. no. 2165), HER3 (Cell Signaling Technology, Inc.; cat. no. 12708), phosphorylated (p-)HER2 (Y1221/1222; Cell Signaling Technology

(16) Effects of N361 Glycosylation on Epidermal Growth Factor Receptor Biological FunctionCancers (Basel)January 31, 2026Dennis Lam, Brandon Arroyo, Ariel N. Liberchuk, Jessica Das, Leonard J. Ash, Khizr M. Khan, Jayati Mondal, Andrew L. Wolfeits corresponding dilutions: 1:100 anti-rabbit monoclonal HER2/ErbB2 (Cell Signaling Technology Cat# 2165, RRID:AB_10692490) and 1:100 anti-mouse monoclonal EGFR (Genetex, Sinchu City, Taiwan, Cat# GTX628887,

(17) Anti-tumor activity of trastuzumab deruxtecan in pediatric solid tumors with variable HER2 expressionMolecular Cancer TherapeuticsJanuary 2, 2026Chelsey M. Burke, Tamar Y. Feinberg, Samantha Brosius, Umeshkumar K. Bhanot, Mala Jain, Irina Linkov, Armaan Siddiquee, Kristina Guillan, Glorymar Ibáñez, Andoyo A. Ndengu, et al.Biosystems, #AR9640, 30 minutes) for primary Anti-ErbB2/HER2 antibody clone 29D8 (Cell Signaling Technology, #2165, RRID:AB_10692490, dilution = 1:800, 30 minutes) and primary Anti-ErbB2/HER2 antibody clone CB11 (Abcam,

(18) Development of Patient-Derived Conditionally Reprogrammed 3D Breast Cancer Culture Models for Drug Sensitivity EvaluationOncology ResearchDecember 30, 2025Jing Cai, Haoyun Zhu, Weiling Guo, Ting Huang, Pangzhou Chen, Wen Zhou, Ziyun Guan9449S, 1:500), anti-ER (Abcam, ab16660, 1:200), anti-PR (Abcam, ab101688, 1:400), and anti-HER2 (CST, 2165S, 1:200), respectively. After incubation with these primary antibodies, sections were treated with a

(19) Prognostic impact of endocrine therapy and BDNF-TrkB axis in breast cancer brain metastasesJ NeurooncolDecember 29, 2025Joori Kim, Kabsoo Shin, Ahwon Lee, Jieun Leephosphor-AKT Ser473 (1:50, GTX50128, Gene Tex), Era (1:100, ab166660, Abcam), and HER2/ErbB2 (1:200, 2165S, Cell Signaling). Quantification of immunofluorescence expression Cell counts of each staining were

(20) Tumor-secreted clusterin promotes cachectic fat wasting via disrupting circadian gene expression and adipogenesisThe EMBO JournalDecember 17, 2025Yan Liu, Yehui Zhou, Mengmeng Zhang, Jin Zhang, Jiahui Chen, Long Chen, Jia Tian, Xiang Lv, Xinxing Ma, Jing Xu, et al.(Cell Signaling Technology, 13258), PR (Cell Signaling Technology, 3172), HER2 (Cell Signaling Technology, 2165), PKP3 (Invitrogen, 35-7600), CLU (Cell Signaling Technology, 42143S), brown, buffy, light yellow, and

Be the First to Write a Review!

HER2/ErbB2 (29D8) Rabbit mAb

HER2/ErbB2 (29D8) Rabbit mAb from Cell Signaling Technology

Description

Questions and Answers

Cell Signaling Technology

Citations (333)

Reviews

HER2/ErbB2 (29D8) Rabbit mAb from Cell Signaling Technology

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map