Western blot analysis of HeLa cell lysate (35ug) by using Rabbit polyclonal anti-RELA antibody at 1:2000 dilution.

Western blot analysis of HT29 cell lysate (35ug) by using Rabbit polyclonal anti-RELA antibody at 1:2000 dilution

Western blot analysis of MCF7 cell lysate (35ug) by using Rabbit polyclonal anti-RELA antibody at 1:2000 dilution.

Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and RELA-Knockout HeLa cells (KO, Cat# LC810201) were separated by SDS-PAGE and immunoblotted with anti-RELA monoclonal antibody TA890002, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.