Fig 1: UME6 expression oscillations during Ca colonization primes protective Th17 immunity(A) Schematic of DOX drinking water supplementation to control UME6 expression by Ca in mice colonized by Ca-2W1S-tetO-UME6.(B) Ca kidney CFUs 5 days after Ca-2W1S intravenous challenge for mice colonized with Ca-2W1S (WT) compared with Ca-2W1S-tetO-UME6 or no colonization controls maintained on ampicillin drinking water without (no DOX) or with (+DOX) continuous supplementation or oscillating DOX supplementation every other day.(C) Number of I-Ab:2W1S CD4+ T cells in the spleen and pooled lymph nodes for mice described in (A) 14 days after Ca oral inoculation.(D) Percentage of RORγt+ and number RORγt + I-Ab:2W1S CD4+ T cells from Ca-2W1S-tetO-UME6 (filled) or WT Ca-2W1S (open) colonized mice described in (C).(E) Percentage of IL-17A- and/or IL-17F- or IFN-γ-producing CD4+ T cells in the spleen and lymph nodes after heat-killed WT Ca stimulation for mice described in (C).(F) Percentage of Tbet+ cells among I-Ab:2W1S CD4+ T cells from Ca-2W1S-tetO-UME6 (filled) or WT Ca-2W1S (open) colonized mice described in (C).Each data point represents the results from an individual mouse, representative of at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Error bars, mean ± SEM. L.o.D., limit of detection.