Anti-ROCK1 antibody ab97592 from Abcam

Product Specs
ItemAnti-ROCK1 antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab97592
Quantity50 µL
IsotypeIgG
Conjugate/TagUnconjugated
ClonalityPolyclonal
HostRabbit
ApplicationsICC/IF, IHC-P, IP, WB
Concentration0.68 mg/ml
Gene NameRock1
ReactivityHuman, Mouse, Rat
NCBI Gene AliasesP160ROCK, ROCK-I
Antibody TypePrimary Antibody
Research AreaOncology
TargetROCK1
NCBI Full Gene NameRho associated coiled-coil containing protein kinase 1

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Abcam

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Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Coordination among cytoskeletal organization, cell contraction, and extracellular matrix development is dependent on LOX for aneurysm preventionThe FEBS JournalDecember 4, 2024Rohtem Aviram, Shelly Zaffryar‐Eilot, Anna Kaganovsky, Anas Odeh, Shay Melamed, Ruslana Militsin, Lavi Coren, Cameron B. Pinnock, Ariel Shemesh, Raz Palty, et al.1 : 1000), anti‐TAGLN (Abcam ab14106, 1 : 1000), anti‐calponin (Abcam ab46794, 1 : 5000), anti‐ROCK1 (Abcam ab97592, 1 : 1000), anti‐ROCK2 (Abcam ab125025, 1 : 3000), anti‐phospho‐MLC2 (Cell Signaling 3674), and anti‐MLC2

(2) Actin Dysregulation Mediates Nephrotoxicity of Cassiae Semen Aqueous ExtractsToxicsJuly 30, 2024Jinlan Yang, Sheng Xiao, Ludi Li, An Zhu, Wusheng Xiao, Qi Wangfrom Sichuan Maccura Biotechnology Co., Ltd. (Chengdu, China). Anti-ROCK1 (phospho T455 + S456; Cat. # ab97592), anti-myosin light chain (phospho S20; Cat. # Ab2480), anti-cofilin (phospho S3; Cat. # ab 12866),

(3) Actomyosin Activity and Piezo1 Activity Synergistically Drive Urinary System Fibroblast ActivationAdvanced science (Weinheim, Baden-Wurttemberg, Germany)November 1, 2023Chen G, Gao X, Chen J, Peng L, Chen S, Tang C, Dai Y, Wei Q, Luo D.from Alomone Laboratories, p‐FAK (ab81298, 1:200 dilution), RhoA (ab187027, 1:200 dilution), ROCK (ab97592, 1:200 dilution), and Col1 (ab138492, 1:200 dilution) from Abcam; α‐SMA (48938, 1:200 dilution), p‐PI3K

(4) Stress stimulation maintaining by genipin crosslinked hydrogel promotes annulus fibrosus healingJournal of orthopaedic translationMay 1, 2023Wang Z, Jin X, Zhang B, Kong J, Deng R, Wu K, Xie L, Liu X, Kang R.stimulation signal pathway-related protein RhoA (1:100, 10749-1-AP, Proteintech, China) and ROCK (1:200, ab97592, Abcam, USA) was performed. Epitopes were quantified using image analysis software (Image-Pro Plus 6.0).

(5) Mechanical stimulation promotes MSCs healing the lesion of intervertebral disc annulus fibrosusFrontiers in bioengineering and biotechnologyJanuary 1, 2023Deng R, Kang R, Jin X, Wang Z, Liu X, Wang Q, Xie L.mechanical stimulation related protein RhoA (1:100, 10749-1-AP, Proteintech, China) and ROCK1 (1:200, ab97592, Abcam, United States) were conducted according to the product instructions and viewed under a microscope

(6) Circ_PIP5K1A regulates cisplatin resistance and malignant progression in non-small cell lung cancer cells and xenograft murine model via depending on miR-493-5p/ROCK1 axisRespiratory ResearchDecember 1, 2021Nan Feng, Zhi Guo, Xiaokang Wu, Ying Tian, Yue Li, Yan Geng, Yan Yuwere blocked and the membranes were incubated with primary antibodies of ROCK1 (Abcam, Cambridge, UK; ab97592, 1:1000) or GAPDH (Abcam, ab128915, 1:1000) at 4 °C overnight. After the incubation of Goat Anti-Rabbit

(7) LncRNA MCM3AP-AS1 sponges miR-148a to enhance cell invasion and migration in small cell lung cancerBMC CancerDecember 1, 2021Hua Luo, Yukun Zhang, Guangmei Qin, Bing Jiang, Lili Miaowere incubated first with primary antibodies against GAPDH (1:1600, ab22555, Abcam) and ROCK1 (1:1600, ab97592, Abcam) at 4 °C for 12 h, then with HRP-labeled goat secondary antibody (IgG) (1:2000; ab6721; Abcam)

(8) Circ_0057558 promotes nonalcoholic fatty liver disease by regulating ROCK1/AMPK signaling through targeting miR-206Cell death & diseaseAugust 26, 2021Chen X, Tan QQ, Tan XR, Li SJ, Zhang XX.ECL kit (Sigma-Aldrich, MO, USA). The following primary antibodies were used: Anti-ROCK1 (1: 1000; ab97592, Abcam, USA); Anti-p-AMPK (1:500; 2535 S, Cell Signaling, USA). Anti-AMPK (1:1000; 2532, Cell Signaling,

(9) miR‑135a‑5p inhibits tumor invasion by targeting ANGPT2 in gallbladder cancerMolecular Medicine ReportsMay 25, 2021Haiyan Diao, Xing Xu, Bin Zhao, Guanghua Yangdilution, 1:5,000; Abcam) and Rho associated coiled-coil containing protein kinase 1 (Rock1; cat. no. ab97592; dilution, 1:500; Abcam). Anti-GAPDH (cat. no. ab9485; dilution, 1:2,500; Abcam) was used as the endogenous

(10) LncRNA‑zinc finger protein 281 downregulates rho‑associated coiled‑coil containing protein kinase 1 by upregulating miR‑144 in osteosarcomaOncology LettersAugust 3, 2020Yuxiong Shao, Zhichao Tong, Jianfeng Wei, Tuanmin YangMerck KGaA) at room temperature. The membranes were probed with rabbit anti-ROCK1 (1:800; cat. no. ab97592; Abcam) and anti-GAPDH (1:800; cat. no. ab37168; Abcam) for 12 h at 4°C, followed by incubation with

(11) Long non-coding RNA CCHE1 participates in postoperative distant recurrence but not local recurrence of osteosarcoma possibly by interacting with ROCK1BMC musculoskeletal disordersJuly 13, 2020Zhang Z, Yu T, Geng W.performed using conventional method. Primary antibodies included rabbit anti-human ROCK1 (1: 1200, ab97592, Abcam) and rabbit antihuman GAPDH antibody (1: 1200, ab37168, Abcam). IgG-HRP (1:1000, MBS435036, MyBioSource)

(12) Down-Regulation of circNRIP1 Promotes the Apoptosis and Inhibits the Migration and Invasion of Gastric Cancer Cells by miR-182/ROCK1 AxisOnco Targets TherJune 30, 2020Lu Liang, Lu Li(ab199677, 1:1000 dilution, Abcam, Cambridge, MA, USA), Bcl-2 (ab196495, 1:5000 dilution, Abcam), ROCK1 (ab97592, 1:2000 dilution, Abcam) at 4°C, and then interacted with HRP- conjugated IgG (ab205718, 1:10,000 dilution,

(13) Down-Regulation of circNRIP1 Promotes the Apoptosis and Inhibits the Migration and Invasion of Gastric Cancer Cells by miR-182/ROCK1 AxisOncoTargets and TherapyJune 30, 2020Lu Liang, Lu Li(ab199677, 1:1000 dilution, Abcam, Cambridge, MA, USA), Bcl-2 (ab196495, 1:5000 dilution, Abcam), ROCK1 (ab97592, 1:2000 dilution, Abcam) at 4°C, and then interacted with HRP- conjugated IgG (ab205718, 1:10,000 dilution,

(14) LncRNA HAND2-AS1 overexpression inhibits cancer cell proliferation in melanoma by downregulating ROCK1Oncology lettersAugust 1, 2019Liu P, Du R, Yu X.temperature for 2 h. The primary antibodies used were rabbit anti-human ROCK1 (1:1,400; catalog no. ab97592; Abcam) and rabbit anti-human GAPDH, (1:1,400; catalog no. ab9485; Abcam) and the incubation was performed

(15) lncRNA HAND2‑AS1 inhibits cancer cell proliferation, migration and invasion by downregulating ROCK1 in HPV‑positive and negative cervical squamous cell carcinomaExperimental and Therapeutic MedicineJuly 30, 2019Liping Jin, Jingquan Ji, Linjie Shi, Shuangling Jin, Lihua Peih at room temperature, the membranes were incubated with antibodies against ROCK1 (1:1,200; cat no. ab97592; Abcam) and GAPDH (1:1,200; cat no. ab37168; Abcam) overnight at 4°C. The membranes were subsequently

(16) lncRNA‑CCHE1 is involved in migration and invasion but not in proliferation of pancreatic adenocarcinoma cells possibly by interacting with ROCK1Oncology LettersMay 30, 2019Xiang Jin, Liping Ye, Minhua Lin, Binbin Gu, Jun Wang, Yequn He, Weilin Lifor 1.5 h. PVDF membranes were incubated with ROCK1 (rabbit anti-human; 1:1,500 dilution; cat. no. ab97592; Abcam, Cambridge, UK) and GAPDH (rabbit anti-human; 1:1,200 dilution; cat. no. ab37168; Abcam) primary

(17) SMAD specific E3 ubiquitin protein ligase�1 promotes ovarian cancer cell migration and invasion via the activation of the RhoA/ROCK signaling pathwayOncology ReportsOctober 31, 2018Wei Wang, Han Du, Huiping Liu, Fangfang Hu, Guangzhi Liuphosphorylated RhoA (1:1,000; cat. no. ab41435), Rho‑associated protein kinase (ROCK1; 1:1,000; cat. no. ab97592) and phosphorylated ROCK1 (1:500; cat. no. ab203273) were obtained from Abcam and incubated overnight

(18) Retracted Article: MiR-182-5p and miR-96-5p increased hepatocellular carcinoma cell mobility, proliferation and cisplatin resistance partially by targeting RND3RSC advancesOctober 10, 2018Yang S, Chen Z, Fan D, Zhang R, Zhang Y, Wu S.for western blotting were purchased from Abcam (Shanghai, China): RND3 (ab171799), MYPT1 (ab32519), ROCK1 (ab97592), ROCK2 (ab228008), PCNA (ab152112), and caspase-3 (ab90437). The following primary antibodies used

(19) MicroRNA-340 Inhibits Epithelial-Mesenchymal Transition by Impairing ROCK-1-Dependent Wnt/β-Catenin Signaling Pathway in Epithelial Cells from Human Benign Prostatic HyperplasiaChinese Medical JournalAugust 20, 2018Si-Yang Chen, Yuan Du, Jian Songrabbit monoclonal antibodies specific to human were employed as primary antibodies including ROCK-1 (ab97592, Abcam, Cambridge, MA, USA) (1:1000), β-catenin (ab32572, Abcam) (1:5000), cyclin D1 (ab134175, Abcam)

(20) Integrin CD11b mediates α-synuclein-induced activation of NADPH oxidase through a Rho-dependent pathwayRedox biologyApril 1, 2018Hou L, Bao X, Zang C, Yang H, Sun F, Che Y, Wu X, Li S, Zhang D, Wang Q.Jose, CA, USA), anti-CD11b (ab128797), anti-α-Synuclein (ab6162), anti-TLR2 (ab108998), anti-ROCK1 (ab97592), anti-GAPDH (ab181602, Abcam, Cambridge, MA, USA) and ionized calcium binding adaptor molecule-1 (Iba1;

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BMC Cancer

Oncology Letters

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Oncology letters

Anti-ROCK1 antibody from Abcam

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Anti-ROCK1 antibody from Abcam

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